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LanthaScreen™ Tb-anti-c-fos [pThr232] Antibody Kit

LanthaScreen® Tb-labeled phospho-specific antibodies, allow for the detection of intracellular post-translational modifications of specific target proteins. To facilitate in-house kinase assay configuration and screening, we have developed a selection of Tb-labeled phospho-specific antibodies, which may be accessed in small scale and screening quantities.

LanthaScreen™ Tb-anti-c-fos [pThr232] Antibody

c-Fos is phosphorylated at Thr232 by mitogen-activated protein kinases (MAPK1 and MAPK3).
For use in combination with the LanthaScreen® c-Fos HeLa cell line (K1579) to evaluate inhibitors in medium to high throughput screening applications.

CellSensor™ c-Fos-bla ME-180 Cell Line

The CellSensor® c-fos-bla ME-180 cell line contains a beta-lactamase reporter gene under control of the c-fos response element stably integrated into ME-180 cells. To construct this cell line, the c-fos-bla construct was transduced into ME-180 cells by lentivirus. Flow cytometry was used to isolate cells responsive to epidermal growth factor (EGF). This cell line is validated for DMSO tolerance, cell number, stimulation time, substrate loading time, as well as Z' and EC50 concentrations for EGF. The CellSensor® c-fos-bla ME-180 cell line is responsive to EGF, HGF, IL-6, OSM, TNFα, IL-1-alpha; and PMA/Thaps, and can be used to probe the JNK/P38/MAPK and JAK/STAT signaling pathways. This cell line can be adapted for high-throughput screening of agonist or antagonist compound libraries. Candidate drugs can also be tested for dose response against this cell line. Academic and non-profit customers, please inquire for special pricing.

Phospho-c-Fos (Thr232) Polyclonal Antibody (Invitrogen™)

Phospho-c-Fos (Thr232) Polyclonal Antibody for Western Blot, IF, ICC, IHC (P), ChIP

CellSensor™ c-Fos-bla HEK293T Cell Line

The c-fos-bla HEK 293T cell line contains a beta-lactamase reporter gene under control of the c-fos promoter stably integrated into HEK 293T cells. This cell line can be used to detect agonists/antagonists of the c-fos pathway. The c-fos-bla HEK 293T cells have been shown to be responsive to phorbol 12-myristate 13-acetate (PMA) stimulation. Academic and non-profit customers, please inquire for special pricing.

Phospho-c-Fos (Thr232) Recombinant Polyclonal Antibody (3HCLC) (Invitrogen™)

Phospho-c-Fos (Thr232) Recombinant Polyclonal Antibody for Western Blot

Phospho-c-Fos (Thr325) Polyclonal Antibody (Invitrogen™)

Phospho-c-Fos (Thr325) Polyclonal Antibody for Western Blot, IF, ICC, ChIP

c-Fos Polyclonal Antibody (Invitrogen™)

c-Fos Polyclonal Antibody for Western Blot

c-Fos Polyclonal Antibody (Invitrogen™)

c-Fos Polyclonal Antibody for Western Blot, IF, ICC

c-Fos Polyclonal Antibody (Invitrogen™)

c-Fos Polyclonal Antibody for IHC (P)

Phospho-c-Fos (Ser362) Polyclonal Antibody (Invitrogen™)

Phospho-c-Fos (Ser362) Polyclonal Antibody for Western Blot, IHC (P)

c-Fos Polyclonal Antibody (Invitrogen™)

c-Fos Polyclonal Antibody for IF, ICC, IHC (P)

Phospho-c-Fos (Ser374) Polyclonal Antibody (Invitrogen™)

Phospho-c-Fos (Ser374) Polyclonal Antibody for Western Blot

Phospho-c-Fos (Ser32) Polyclonal Antibody (Invitrogen™)

Phospho-c-Fos (Ser32) Polyclonal Antibody for Western Blot

c-Fos Recombinant Rabbit Monoclonal Antibody (RM374) (Invitrogen™)

c-Fos Recombinant Monoclonal Antibody for Western Blot, IHC