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CellSensor™ T-REx™ FOXO3 DBE-bla HeLa Cell Line

The CellSensor® T-REx™ FOXO3 DBE-bla HeLa cell line was engineered by lentiviral transduction of HeLa cervical cancer cells with a FOXO3 response element driving beta-lactamase expression (DBE-bla), along with a tetracycline repressor and tetracycline (or the tetracycline analog doxycycline)-inducible FOXO3 constructs. Addition of doxycycline to these cells allows for FOXO3 transcription factor expression and subsequent beta-lactamase expression, which in turn can be down-regulated by insulin-mediated activation of the PI3K/AKT signaling pathway which leads to phosphorylation and inactivation of FOXO3 and concomitant suppression of beta-lactamase expression. This cell line has been tested for robust assay performance by assessing a variety of assay parameters, including: cell plating number, DMSO tolerance, stimulation time, and substrate loading time. Assay validation was performed using serial dilutions of insulin to antagonize doxycycline- induced FOXO3 activity (Figure 1). Additional testing data using RNAi and alternate stimuli are also available.

Phospho-FOXO1/FOXO3/FOXO4 (Thr24, Thr32) Polyclonal Antibody (Invitrogen™)

Phospho-FOXO1/FOXO3/FOXO4 (Thr24, Thr32) Polyclonal Antibody for IHC (P)

FOXO1/FOXO3 Polyclonal Antibody (Invitrogen™)

FOXO1/FOXO3 Polyclonal Antibody for IHC (P)

FOXO1/FOXO3/FOXO4 Polyclonal Antibody (Invitrogen™)

FOXO1/FOXO3/FOXO4 Polyclonal Antibody for IHC (P)

Phospho-FOXO1/FOXO3 (Ser322, Ser325) Polyclonal Antibody (Invitrogen™)

Phospho-FOXO1/FOXO3 (Ser322, Ser325) Polyclonal Antibody for Western Blot

FOXO1/FOXO3/FOXO4 Polyclonal Antibody (Invitrogen™)

FOXO1/FOXO3/FOXO4 Polyclonal Antibody for Western Blot, IHC (P)

Phospho-FOXO1/FOXO3/FOXO4 (Thr24, Thr32) Polyclonal Antibody (Invitrogen™)

Phospho-FOXO1/FOXO3/FOXO4 (Thr24, Thr32) Polyclonal Antibody for Western Blot

FOXO3 Monoclonal Antibody (OTI3E6), TrueMAB™ (OriGene)

FOXO3 Monoclonal Antibody for Western blot

FOXO3 Monoclonal Antibody (OTI6H9), TrueMAB™ (OriGene)

FOXO3 Monoclonal Antibody for Western blot

FOXO3 Monoclonal Antibody (OTI5F11), TrueMAB™ (OriGene)

FOXO3 Monoclonal Antibody for Western blot, IHC (P)

FOXO3 Monoclonal Antibody (OTI5F11), TrueMAB™ (OriGene)

FOXO3 Monoclonal Antibody for Western blot, IHC (P)

FOXO3 Monoclonal Antibody (OTI6H9), TrueMAB™ (OriGene)

FOXO3 Monoclonal Antibody for Western blot

FOXO3 Monoclonal Antibody (OTI3E6), TrueMAB™ (OriGene)

FOXO3 Monoclonal Antibody for Western blot

FOXO3A Polyclonal Antibody (Invitrogen™)

FOXO3A Polyclonal Antibody for Western Blot, IF, ICC

FOXO3A Polyclonal Antibody (Invitrogen™)

FOXO3A Polyclonal Antibody for Western Blot, IF, ICC