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FRAP1 (mTOR) Recombinant Human Protein

FRAP1 (mTOR) is a serine⁄threonine kinase that plays a critical role in cellular growth and proliferation. Perturbations in the mTOR⁄PI3-kinase⁄AKT pathway are associated with numerous forms of cancer. FRAP1 is also the target of rapamycin and its analogues, which are currently used as immunosuppressants and cancer therapeutics.

FRAP1 (mTOR) Assay Buffer

1X Assay Buffer for use with the LanthaScreen® FRAP1 (mTOR) assays.

mTOR Polyclonal Antibody (Invitrogen™)

mTOR Polyclonal Antibody for Western Blot, IF, ICC

mTOR Polyclonal Antibody (Invitrogen™)

mTOR Polyclonal Antibody for Western Blot, IF, ICC, IP

Phospho-mTOR (Ser2448) Polyclonal Antibody (Invitrogen™)

Phospho-mTOR (Ser2448) Polyclonal Antibody for Western Blot, IF, ICC

Rapamycin (Invitrogen™)

Rapamycin is a member of a family of macrolide immunosuppressants that bind to and inhibit the FK506 binding protein (FKBP) proline rotamase.

Format/Formulation: Lyophilized

mTOR Antibody (7H13L18), ABfinity™ Rabbit Monoclonal (Invitrogen™)

mTOR Monoclonal Antibody for Western Blot, IF, ICC

mTOR Antibody (7HCLC), ABfinity™ Rabbit Oligoclonal (Invitrogen™)

mTOR Oligoclonal Antibody for Western Blot, IF, ICC, ChIP

mTOR Monoclonal Antibody (215Q18) (Invitrogen™)

mTOR Monoclonal Antibody for Western Blot, IF, ICC, IHC (P)

LanthaScreen™ Akt HEK 293E Cell Line

LanthaScreen® GFP Cellular Assays allow for the analysis of post-translational modifications for a number of target proteins in an live-cell context. The PI3K⁄AKT pathway mediates signals for cell growth, cell survival, transcription, translation, and glucose uptake. Because of the complexity of this signaling cascade, especially as applied to the regulation of the mammalian target of rapamycin (mTOR), cell-based methods are critical for proper identification of small-molecule mediators of this pathway. The significance of mTOR as a kinase has been underscored recently by the identification of two distinct multimeric complexes inside the cell: mTORC1 (includes raptor and is rapamycin sensitive) and mTORC2 (includes rictor and is insensitive to acute rapamycin exposure). mTORC2 has been shown to phosphorylate and AKT at residue Ser473 for complete activation of this prosurvival kinase. LanthaScreenTM AKT HEK293E is a human cell line which constitutively expresses GFP-AKT fusion proteins. This kinase target was introduced using lipid transfection and these cells are a clonal population isolated by FACS, using GFP fluorescence as a sorting marker and Blasticidin to maintain cells under selection. Using this cell line, a homogenous immunoassay was developed with a time-resolved FRET (TR-FRET) readout in which the insulin-induced phosphorylation of Ser473 on GFP-AKT is detected in cell lysates using a terbium-labeled phosphor-specific antibody. This cell line has been validated with different stimuli⁄inhibitors and shows correct EC50 ⁄ IC50 values. Moreover, this assay has been optimized for performance under variable experimental conditions (including cell plating density, agonist stimulation time, DMSO tolerance and assay development time) and displays excellent statistical data (Z' > 0.6) and good signal-to-background.

mTOR Polyclonal Antibody (Invitrogen™)

mTOR Polyclonal Antibody for Western Blot, ICC

mTOR Polyclonal Antibody (Invitrogen™)

mTOR Polyclonal Antibody for Western Blot, IF, ICC, IHC (P)

mTOR Polyclonal Antibody (Invitrogen™)

mTOR Polyclonal Antibody for Western Blot, IP

mTOR Polyclonal Antibody (Invitrogen™)

mTOR Polyclonal Antibody for Western Blot, IF, ICC, IHC (P), IP, ChIP