Documents & Support

1 - 15 of 22 results

What is the importance of gamma-irradiated sera? Product FAQ

Answer

Gamma irradiation is recognized as an effective method for inactivating viruses in animal-origin material. Based on USDA regulations for the general requirements for antibody products (9CFR, Section 113.450), the minimum dosage for blood derivatives of animal origin is 25 kGy. Certain European countries require products to be treated prior to importation with a minimum dose of 25 kGy. We will gamma-irradiate serum on request. We have validated a process for utilizing gamma irradiation in the range of 30-45 kGy to inactivate the most common bovine viruses and mycoplasmas that may be present in FBS. The level of inactivation is 6-8 logs for viruses and 6-7 logs for mycoplasmas. We have also demonstrated that physiochemical properties and cell culture performance of serum is not altered by gamma irradiation at levels of 30-45 kGy.

Answer Id:: E11869

Was this answer helpful?

Yes No

Thank you for your response

Brochure: Gibco Cell Culture Sera for Bioprocessing Product Literature

Can I get custom serum products for my specific research requirements? Product FAQ

Answer

Yes. We offer a wide range of custom options. Please contact techsupport@thermofisher.com to find your Sera Specialists to discuss your application and research needs. We will work with you to supply sera that is specially processed and tested to meet your individual requirements. Some examples of our custom capabilities are heat inactivation, gamma irradiation, and testing for tetracycline residues.

Answer Id:: E11868

Was this answer helpful?

Yes No

Thank you for your response

Does serum concentration have an inhibitory effect on the luciferase assay? Product FAQ

Answer

For Fetal Bovine Serum (FBS), there is no difference between 10% and 5% FBS. It is worthwhile to note that the type of serum does affect the luciferase assay. We tested the effect of 6 different types of sera (FBS, heat-inactivated FBS, dialyzed FBS, charcoal-treated FBS, donor adult bovine serum, and donor equine serum) on secreted luciferases, e.g. Gaussia, Gaussia-Dura, and Cypridina Luciferases. No noticeable difference was observed among different types of sera except for donor adult bovine serum. Unknown factors in donor adult bovine serum caused up to 35% inhibition in secreted luciferase activity. Therefore, we don’t recommend using donor adult bovine serum for Gaussia, Gaussia-Dura, and Cypridina Luciferases.

Answer Id:: E15655

Was this answer helpful?

Yes No

Thank you for your response

How can I minimize lot-to-lot variability in serum? Product FAQ

Answer

You can test each new lot to be sure that it works for your specific applications. Alternatively, our serum sales team can determine the most consistent, highest-performing serum lot available using the Gibco iMatch Sera Lot Matching Tool.

Answer Id:: E18578

Was this answer helpful?

Yes No

Thank you for your response

Brochure: Gibco Sera Guide Product Literature

When should I use gamma irradiated serum? Product FAQ

Answer

Gamma irradiation is generally recognized by regulatory agencies as the best method of virus inactivation and for reducing the risk of viruses that are naturally present in animal sera. We offer a small range of gamma-irradiated products ex-stock. Gamma irradiation is also available on request as a custom service for most standard catalog products. We offer a range of fully validated custom processes covering many dose ranges.

Answer Id:: E11870

Was this answer helpful?

Yes No

Thank you for your response

Is precipitation in Gibco Certified Fetal Bovine Serum normal? Product FAQ

Answer

Since our sera are not pre-aged before filtration, turbidity or flocculent debris may develop during thawing or storage. This flocculence is a normal characteristic of the serum and does not adversely affect performance characteristics of the serum.

Answer Id:: E19913

Was this answer helpful?

Yes No

Thank you for your response

Is it normal to have precipitation in Fetal Bovine Serum, Tet system approved? Product FAQ

Answer

Since our sera are not pre-aged before filtration, turbidity or flocculent debris may develop upon thawing or storage. This flocculence is a normal characteristic of the serum and does not adversely affect performance of the serum.

Answer Id:: E19362

Was this answer helpful?

Yes No

Thank you for your response

Increased proliferation of human synovial mesenchymal stem cells with autologous human serum: comparisons with bone marrow mesenchymal stem cells and with fetal bovine serum. Citations & References

  • Authors: Nimura A, Muneta T, Koga H, Mochizuki T, Suzuki K, Makino H, Umezawa A, Sekiya I
  • Journal: Arthritis Rheum
  • PubMed ID: 18240254

How much serum should I add to my media? Product FAQ

Answer

The serum concentration will vary with the cell line and basal medium used. Please go here to see our recommended sera supplementations for tested cell lines (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/classical-media/advanced-d-mem-and-mem/recommended-sera-supplementations.html).

Answer Id:: E11862

Was this answer helpful?

Yes No

Thank you for your response

Will improper storage of my tissue culture reagents affect the growth rate of my culture? Product FAQ

Answer

Yes. Store animal sera at -5 to -20 degrees C. Store media at 2 to 8 degrees C; use within recommended shelf life period. Store complete media (supplemented) at 2 to 8 degrees C, and for complete medium the recommended shelf life is 2 to 4 weeks. Additionally, minimize exposure of sera and media to light.

Answer Id:: E11825

Was this answer helpful?

Yes No

Thank you for your response

Inhibition of nonspecific binding of fluorescent-labelled antibodies to human eosinophils. Citations & References

  • Authors: Mahmudi-Azer S, Lacy P, Bablitz B, Moqbel R
  • Journal: J Immunol Methods
  • PubMed ID: 9776581
Catalog # B2752

The role of serum and serum components in the merocyanine 540-sensitized photoinactivation of K562 leukemia cells. Citations & References

  • Authors: Gaffney DK, Sieber F
  • Journal: Biochim Biophys Acta
  • PubMed ID: 1420282
Catalog #
  • M24571(Discontinued)
  • M299
Results per page
spinner