Product FAQ

What is the difference between Fetal Bovine Serum and Fetal Calf Serum?

Answer

There is no difference in the general use of the terms. Fetal Bovine Serum (FBS) was once known as Fetal Calf Serum (FCS).

Answer Id: E11865

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Product FAQ

Has Fetal Bovine Serum, qualified, USDA-approved regions (Cat. Nos. 10437010, 10437028, 10437036, A3160601, A3160602) been heat inactivated? Do I need to heat inactivate it?

Answer

Fetal Bovine Serum, qualified, USDA-approved regions (Cat. Nos. 10437010, 10437028, 10437036, A3160601, A3160602) has not been heat inactivated. We would only recommend heat-inactivating it if your application specifically requires it. We recommend using heat-inactivated serum when working with immune type cells or for immunological applications.

Answer Id: E17438

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Product FAQ

How should I thaw FBS so that the performance is not compromised?

Answer

Remove serum from the freezer and allow it to thaw in the refrigerator at 2-8 degrees C overnight. The thawing process may then be completed at room temperature. The serum must be regularly mixed during this process.

Warning: Do not incubate FBS at 37 degrees C for extended periods of time. The product may become cloudy, and performance could be affected due to the sensitivity of many serum components. Once it is thawed, you can use the product immediately.

Answer Id: E11866

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Product FAQ

Will fetal bovine serum interfere with the Rapid ELISA Mouse Antibody Isotyping kit?

Answer

No, the kit is compatible with FBS; the kit antibodies do not cross-react with bovine immunoglobulins.

Answer Id: E8657

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Product FAQ

Can I heat inactivate the serum in the new One Shot FBS bottle?

Answer

Yes, the serum in the new One Shot FBS bottle can be heat inactivated at 56 degrees C for 30 minutes.

Answer Id: E14693

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Product FAQ

What is the recommended storage temperature for your FBS products?

Answer

We recommend storing our FBS products at ≤-10 degrees C. The current United States Pharmacopeia (USP) and European Pharmacopoeia (EP) guidelines for monographs for serum refer to a storage temperature of ≤-10 degrees C.

While our previous recommended storage temperature of -5 to -20 degrees C have complied with this range, the change to ≤-10 degrees C ensures that the storage temperature range on the label and CoA is consistent with USP and EP requirements.

Answer Id: E16476

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Product FAQ

What is the shelf life of Fetal Bovine Serum, qualified, United States once thawed? Can I refreeze it after thawing?

Answer

The stability after opening and thawing is application-dependent and most often, it is used within a week or less. The product can be thawed and refrozen. This is standard practice for making aliquots. We recommend that you make smaller aliquots and use as needed.

Answer Id: E17384

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SDS

Fetal Bovine Serum Dialyzed (US)

Catalog #
  • 26400036(Discontinued)
  • SM10002(Discontinued)
  • SM10006(Discontinued)
  • SP10001(Discontinued)

Product FAQ

What is BVD virus (BVDV) in fetal bovine serum and how does it apply to cell culture applications?

Answer

BVD stands for Bovine Viral Diarrhea. It is one of the most common viral infections in cattle. It is estimated that 70 to 90 percent of the world's cattle population is seropositive for BVD. This virus can cause abnormalities and fetal abortions in cattle. Several strains of BVD exist, some of which are non-pathogenic.

Bovine serum is tested in accordance with 9 CFR, Section 113.53. The BVDV fluorescent antibody test is one of the required tests to meet Title 9 part 113.53 of the Code of Federal Regulations. However, the results of this test are somewhat subjective in the way they are scored. They are scored 0 to +4, based on the level of observed fluorescence. Most samples will have a detectable level of BVDV antigen due to its prevalence in the bovine population. The FA part of the testing can reveal the presence of non-cytopathic BVD strains. The Cytopathogenic and Hemadsorbing Agents testing is used to determine the release of bovine serum, regardless of the BVDV result. We currently report the BVDV results as “Tested”. Any live cytopathic bovine viruses (including the cytopathic BVDV strain) would be revealed in the testing for cytopathogenic agents. In this portion of the testing, the cultures are microscopically monitored for evidence of inclusion bodies, abnormal number of giant cells, or other cytopathology indicative of cell abnormalities. The hemadsorption assay detects the presence of hemagglutinating viruses. The viral hemagglutinin would induce clumping of red blood cells. If a positive result in the cytopathogenic agents or hemadsorbing agents assays is reported, the material would be failed and therefore would not be released by Quality Assurance.

Answer Id: E11874

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Product FAQ

Do I have to heat-inactivate Fetal Bovine Serum, charcoal stripped, USDA-approved regions, One Shot format (Cat. No. 12676029 or A3382101)?

Answer

The material is not heat-inactivated. We would only recommend heat-inactivating it if your application specifically requires it. We recommend using heat-inactivated serum when working with immune type cells or for immunological applications.

Answer Id: E17393

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Product FAQ

Will fetal bovine serum (FBS) in a sample interfere with the Easy-Titer Antibody assay?

Answer

No, these assay use beads with target-specific antibodies, so off-target immunoglobulins have little or no effect on assay performance.

Answer Id: E8631

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Product FAQ

I am happy with my present media, even though I am supplementing it with 10% FBS. Why would I change to Advanced media?

Answer

The Advanced media allow a reduction in serum usage from 50 to 90%. In the majority of cell lines, a 50% reduction can be achieved with no weaning procedure. Further reductions can be realized by weaning cells gradually. The amount of reduction in serum percentage beyond 50% will be cell line dependent. The advantages of using less serum include cost savings, particularly during those times when serum prices increase, extending the life of your serum lot, and reduced variability. For more information on the Advanced DMEM and Advanced MEM, please search “Advanced Media” from our website home page.

Answer Id: E11864

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Product FAQ

What is the shelf life for One Shot FBS?

Answer

The current shelf life for One Shot FBS products is 2 years from the date of manufacture.

Answer Id: E16477

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Product FAQ

What is the shelf life of the new One Shot FBS?

Answer

The initial shelf life/stability for the new One Shot FBS is two years from the date of manufacture. Additional updates will continue to be made as data becomes available.

Answer Id: E14694

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Product FAQ

What type of plastic is the new One Shot FBS bottle made from?

Answer

It is made from polyethylene terephthalate (PET, PETE). This is the same plastic used in our other bottles.

Answer Id: E14691

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