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Brochure: Titan3 and Target2 Chromatography Syringe Filters – Performance Filtration Solutions for Chromatographic Applications Product Literature

Can I filter sterilize Essential 8 Medium using a 0.22 µm filter? Product FAQ

Answer

You can filter the basal Essential 8 medium or the complete Essential 8 medium through a 0.22 µm filter (vacuum driving filtration). But, we recommend that you use a low protein-binding filter.

Answer Id:: E17399

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Sometimes the ExpiCHO cell supernatant is difficult to filter during clarification. How can I overcome this? Product FAQ

Answer

Because the ExpiCHO system uses components that differ from that of HEK 293-based expression systems such as the Gibco Expi293 system, the supernatant may be more difficult to process through standard bottle top filters in some instances. To remedy this, we recommend centrifuging the supernatant first at ~5,000 x g for 30 minutes followed by filtration using a 0.22 µm filter. Alternative filters (such as depth filters) provide superior filtration for supernatants, especially at larger scales, as these filters are specifically designed to handle supernatant from CHO-derived expressions.

Answer Id:: E14746

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Recovery of Francisella tularensis from soil samples by filtration and detection by real- time PCR and cELISA Citations & References

  • Authors: Sellek, R; Jimenez, O; Aizpurua, C; Fernandez-Frutos, B; De Leon, P; Camacho, M; Fernandez-Moreira, D; Ybarra, C; Cabria, JC
  • Journal: JOURNAL OF ENVIRONMENTAL MONITORING

How should I filter sterilize CD Hybridoma Medium? Product FAQ

Answer

CD Hybridoma Medium can be filtered by 0.2 µm filtration.

Answer Id:: E17398

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How do I use Thermo Scientific Nalgene glass fiber prefilters? Product FAQ

Answer

Glass fiber prefilters are available as an optional accessory (DS0281 series) and bundled with select Thermo Scientific Nalgene Rapid-Flow filter units. They are useful in filtration of solutions with an especially high particulate load, often enabling a higher volume to be filtered before significant flow decay by trapping and preventing the larger particulate matter from entering the final filter membrane. Simply place one prefilter on top of the membrane bonded inside the unit, wet slightly to hold in place, and proceed with filtration as usual.

Answer Id:: E15967

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Do I need to filter the serum before use? Product FAQ

Answer

No, there is no need to filter the serum before use. Gibco serum products are manufactured using the most stringent processes, including membrane filtration.

Answer Id:: E11867

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