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Our diverse collection of antibodies, assays, beads and buffers support leading research areas. Antibodies NovaFluor dye–conjugated antibodies Brilliant Ultra Violet and Brilliant Violet Antibodies Assays and reagents Beads for compensation, instrument controls, and standards 5 steps to...

Find valuable information. Optimize your experiments to get the best results. We’ve compiled a detailed knowledgebase of the top tips and tricks to meet your research needs. View the relevant questions below:, Browse our FAQ database for more information ›, Need more information?

Herein we provide guidance on how to select fluorophores and other reagents for spectral flow cytometry. Design and build spectral panel Spectral flow cytometry fundamentals, Advances in flow cytometry instrumentation coupled to a growing number of fluorescent labels and readouts have expanded...

(See a list of the products featured in this article.) Whether in academic, commercial, or government laboratories, researchers analyzing very large numbers of samples routinely endure time-intensive assays, a requirement to physically monitor the instruments, and susceptibility to human error.

Blood collection tubes with anticoagulant, either heparin, K3EDTA, or K2EDTA tubes 12 x 75 mm round-bottom polystyrene tubes (e.g., Thermo Scientific Samco 12 x 75 mm Disposable Culture Tubes, Cat. No. 12-007S) Flow cytometry staining buffer (e.g.

These videos, tutorials and webinars are designed to provide technical and education information regarding flow cytometers as well as flow cytometry research applications and techniques. Learn more about the Attune NxT Flow Cytometer, With the release of 21 CFR Part 11 compliance ready software for...

Download PDF version Dose response curves are a standard research technique used to evaluate therapeutic efficacy of cancer drugs. The use of flow cytometry enables accurate simultaneous evaluation of multiple analytes, in a statistically relevant sample size in a short time.

Herein we discuss the basics of spectral flow cytometry data analysis including data generation, visualization, and data mining. This is the last step in the Spectral Flow Cytometry experimental process ( Figure 1 ).

Flow cytometry provides a rapid method to quantify cell characteristics. However, most flow cytometers cannot directly provide the cell concentration or absolute count of cells in a sample. Absolute cell counts have been widely used in quantifying cell populations and disease progression research,...

View webinars as well as full transcripts from researchers on flow cytometry topics including experimental setup, compensation, and data analysis. We've also got webinars presented by our experienced technical support scientists that provide tips and tricks and offer guidance for selecting the best...

(See a list of the products featured in this article.) High-throughput screening (HTS) is an extremely effective method for identifying potential candidates for therapeutics. Initial compound screening is often performed on a microplate reader because multiwell plate assays tend to be easy to...

The ability to stain live cells with a viability dye and preserve that staining pattern after fixation is critical for intracellular immunophenotyping. Exclusion of the dead cells from the data allows cleaner separation and identification of cell populations.

Rare event analysis started more than thirty years ago when Cupp aimed to count fetal red blood cells in maternal circulation [1]. Nowadays, rare events analysis has assumed growing importance for the diagnosis and monitoring of immunological and hematological disorders.

Download PDF version The ability to direct human pluripotent stem cells (hPSCs) towards differentiated cell phenotypes offers tremendous potential for personalized and regenerative medicine [1,2]. The identification of key transcriptional regulators of pluripotency, as well as chemically defined...

Having difficulties with your experiment? We are dedicated to your success. Get back on track. View our expert recommendations for commonly encountered problem scenarios. View the relevant questions below:, Browse our FAQ database for more information ›, Need more information?