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Alternatives to current flow cytometry data analysis for clinical and research studies. Citations & References

  • Authors: Gondhalekar C, Rajwa B, Patsekin V, Ragheb K, Sturgis J, Robinson JP
  • Journal: Methods
  • PubMed ID: 29305968

Paraformaldehyde fixation of hematopoietic cells for quantitative flow cytometry (FACS) analysis. Citations & References

  • Authors: Lanier LL, Warner NL
  • Journal: J Immunol Methods
  • PubMed ID: 7310138
Catalog # P846

Can the LIVE/DEAD Fixable Dead Cell Stain kits be used with spheroids? Product FAQ

Answer

No. They are intended for use on cell suspensions for flow cytometry analysis.

Answer Id:: E18291

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Flow cytometry analysis of alpha1-adrenoceptor subtypes. Citations & References

  • Authors: Hirasawa A, Tsumaya K, Awaji T, Shibata K, Homma N, Shinomiya T, Tsujimoto G
  • Journal: FEBS Lett
  • PubMed ID: 8647269
Catalog # B7433

Is an isotype control a must for flow cytometry analysis? Product FAQ

Answer

No. Many users are using unstained cells in combination with FMO controls to identify their positive populations

Answer Id:: E14840

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Can I fix my CellEvent Caspase 3/7 - labeled cells? Product FAQ

Answer

For imaging applications, the CellEvent product signal may be retained after fixation in 3.7% formaldehdye for 15 min. Fixation is not recommended for flow cytometry analysis.

Answer Id:: E14994

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Can I use the Human Neural Stem Cell Immunocytochemistry Kit for flow cytometry analysis? Product FAQ

Answer

We do not recommend using the Human Neural Stem Cell Immunocytochemistry Kit for flow cytometry analysis. The kit and protocol were specifically developed for imaging analysis (microscopy/high-content screening (HCS)).

Answer Id:: E19022

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Flow cytometry analysis of the expression of neutrophil FMLP receptors. Citations & References

  • Authors: Allen CA, Broom MF, Chadwick VS
  • Journal: J Immunol Methods
  • PubMed ID: 1593131
Catalog #
  • P852
  • F1314(Discontinued)

Can I use the Human Cardiomyocyte Immunocytochemistry Kit for flow cytometry analysis? Product FAQ

Answer

We do not recommend using the Human Cardiomyocyte Immunocytochemistry Kit for flow cytometry analysis. The kit and protocol were specifically developed for imaging analysis (microscopy/high-content screening (HCS)).

Answer Id:: E19016

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Can I use the Human Dopaminergic Neuron Immunocytochemistry Kit for flow cytometry analysis? Product FAQ

Answer

We do not recommend using the Human Dopaminergic Neuron Immunocytochemistry Kit for flow cytometry analysis. The kit and protocol were specifically developed for imaging analysis (microscopy/high-content screening (HCS)).

Answer Id:: E19019

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Can I use the Pluripotent Stem Cell Immunocytochemistry Kit for flow cytometry analysis? Product FAQ

Answer

We do not recommend using the Pluripotent Stem Cell Immunocytochemistry Kit for flow cytometry analysis. The kit and protocol were specifically developed for imaging analysis (microscopy/high-content screening (HCS)).

Answer Id:: E19005

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What quality controls are used to ensure product performance for the PSC Definitive Endoderm Induction Kit? Product FAQ

Answer

Each lot is tested for % efficiency of definitive endoderm induction using flow cytometry analysis. Additionally, it is tested for sterility, endotoxin, pH, and osmolality.

Answer Id:: E9370

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Can I use the Pluripotent Stem Cell 4-Marker Immunocytochemistry Kit for flow cytometry analysis? Product FAQ

Answer

We do not recommend using the Pluripotent Stem Cell 4-Marker Immunocytochemistry Kit for flow cytometry analysis. The kit and protocol were specifically developed for imaging analysis (microscopy/high-content screening (HCS)).

Answer Id:: E19002

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How do I titrate an antibody for flow cytometry analysis? Product FAQ

Answer

-When you titrate a new antibody, we recommend looking at a range of concentrations above and below the recommended amount, perhaps 0.5-20 µL.
-Plot your data on a histogram, and select the concentration where the separation of the mean fluorescence intensity of the positive and negative populations is the greatest.
-The negative can either be the negative peak if you are looking at a mixed population, or an isotype control.
-If you use too little antibody, the separation is small because the positive population does not get fully labeled.
-If you use too much antibody, the separation decreases because you start to get non-specific binding which moves the negative population toward the positive population.
-If the suggested range does not give you one or the other of these extremes, you may need to extend the titration with more or less antibody.

Answer Id:: E14803

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Using phytoplankton and flow cytometry to analyze grazing by marine organisms. Citations & References

  • Authors: Cucci TL, Shumway SE, Brown WS, Newell CR
  • Journal: Cytometry
  • PubMed ID: 2776582
Catalog # M8997
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