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What is compensation? Product FAQ

Answer

Compensation is a technique used to eliminate false signal that results from spectral overlap between fluorescent dyes when used in multicolor staining panels. For example, FITC has a broad emission spectra that spills into the PE detector. As a result, the cytometer will register a false population of PE labeled cells. Corrections must be made to avoid this. For further information on compensation visit this website: https://www.thermofisher.com/us/en/home/life-science/cell-analysis/flow-cytometry/flow-cytometry-calibration/flow-cytometry-compensation-tools.html#001.

Answer Id:: E14448

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What is flow cytometry? Product FAQ

Answer

Cytometry is the measurement of physical or chemical characteristics of cells or particles. Flow cytometry measures these characteristics of cells or particles as they individually pass lasers in a flow cytometer instrument. Flow cytometry is performed on single cells, providing discrete measurements for each cell in the sample. It also provides a statistical distribution of the measured characteristics of the sample.

A flow cytometer is made up of three subsystems: fluidics, optics, and electronics. Fluidics moves the cells and introduces them for interrogation. Optics generates and collects the light signals. Electronics converts the optical signals to proportional electronic signals for computer analysis.

Answer Id:: E14796

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What are the advantages of acoustic-assisted hydrodynamic focusing in flow cytometry? Product FAQ

Answer

-Modular design - Multiple configurations available - field upgradable.
-Save time - 10X faster speeds with no loss in data quality.
-Simplified sample prep - No wash, no lyse options, non-clogging fluidics.
-Enables unique applications - Complex protocols on a broad range of cell types and samples.

Answer Id:: E14800

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An adapter for defined sample volumes makes it possible to count absolute particle numbers in flow cytometry. Citations & References

  • Authors: Schweppe F, Hausmann M, Hexel K, Barths J, Cremer C
  • Journal: Anal Cell Pathol
  • PubMed ID: 1356415
Catalog # M8997

Characterizing aquatic bacteria according to population, cell size, and apparent DNA content by flow cytometry. Citations & References

  • Authors: Robertson BR, Button DK
  • Journal: Cytometry
  • PubMed ID: 2465113
Catalog #
  • A3568
  • A1301
  • E3565(Discontinued)
  • E1305(Discontinued)

Polyploid and hybrid origins of pacific island sandalwoods (Santalum, santalaceae) inferred from low-copy nuclear and flow cytometry data Citations & References

  • Authors: Harbaugh, DT
  • Journal: INTERNATIONAL JOURNAL OF PLANT SCIENCES

Flow Cytometry Reference Standards—Section 23.2 Molecular Probes Handbook

Antifades and Other Tools for Fluorescence Applications—Chapter 23 Molecular Probes Handbook

Quantitative Flow Cytometry Measurements in Antibodies Bound per Cell Based on a CD4 Reference. Citations & References

  • Authors: Wang L, Degheidy H, Abbasi F, Mostowski H, Marti G, Bauer S, Hoffman RA, Gaigalas AK
  • Journal: Curr Protoc Cytom
  • PubMed ID: 26742654

How is the Attune NxT Acoustic Focusing Cytometer different from traditional flow cytometers? Product FAQ

Answer

With the option to be configured with up to 4 lasers and 14 colors for multi-parameter analysis the Attune NxT Acoustic Focusing Cytometer was designed as a modular system to fit most experimental needs and lab budgets. The novel design of the optical path helps ensure precise fixed alignment of four spatially separated lasers onto the sample stream enabling consistency in data over time, superior performance, and superior reliability. The instrument can be configured with up to 4 solid-state lasers (405 nm, 488 nm, 561 nm, and 637 nm) with flat top beam profiles.

The Attune NxT Flow Cytometer's acoustic focusing uses ultrasonic radiation pressure (> 2 MHz) to transport particles into the center of the sample stream. This pre-focused stream is then injected into the sheath stream, which supplies an additional hydrodynamic pressure to the sample. The combination of these two forces- termed acoustic-assisted hydrodynamic focusing-results in a narrow core stream and uniform laser illumination, regardless of the sample input rate. In traditional cytometers that rely solely on hydrodynamic focusing, the sample core widens to accommodate the increases in flow rate, which results in less uniform laser light illumination.

Answer Id:: E14799

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What is the Attune NxT Autosampler? Product FAQ

Answer

The Attune NxT Autosampler, an optional accessory for the Attune NxT Acoustic Focusing Cytometer, enables rapid processing of up to 384 samples. It has broad compatibility with different plate formats, both 96- and 384-well plates. It has an intelligent probe designed to minimize clogging and carryover (<0.5%) and to prevent damage to the instrument. It mixes by aspiration rather than shaking to ensure homogeneity of the sample and maintain cell viability. Is performs automated cleaning as part of the shutdown process of the Attune NxT Cytometer. It provides consistent data regardless of sampling method (tube vs. plate) and collection rate.

Answer Id:: E14801

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What is the smallest size that I can detect with the Attune NxT Acoustic Focusing Cytometer? Product FAQ

Answer

The smallest size that you can detect with the Attune NxT Acoustic Focusing Cytometer is 0.5 µm.

Answer Id:: E14802

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Flow Cytometry Protocols Experiment Protocol

Guidelines for performing single-platform absolute CD4+ T-cell determinations with CD45 gating for persons infected with human immunodeficiency virus. Centers for Disease Control and Prevention. Citations & References

  • Authors: Mandy FF, Nicholson JK, McDougal JS
  • Journal: MMWR Recomm Rep
  • PubMed ID: 12583540

Assays for Cell Enumeration, Cell Proliferation and Cell Cycle—Section 15.4 Molecular Probes Handbook

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