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Compensation beads capture species-specific antibodies conjugated to fluorophores and other types of reagents. The purpose of these beads is to set voltages and gating parameters for obtaining accurate fluorescence signal.

(See a list of the products featured in this article.) The discovery in the early 1960s [1] and subsequent development of Green Fluorescent Protein (GFP) as a reporter gene has greatly advanced the study of gene expression, protein localization, and cell and tissue development in a multitude of...

Flow cytometry is used to obtain quantifiable results in reference to the physical characteristics of single cells. Microspheres are small, non-biological beads that can be used as a (1) standard for flow cytometer instrument calibration, (2) reference for cell or particle size, and/or (3)...

The ability to stain live cells with a viability dye and preserve that staining pattern after fixation is critical for intracellular immunophenotyping. Exclusion of the dead cells from the data allows cleaner separation and identification of cell populations.

Ensure optimal flow cytometer instrument performance by using Thermo Scientific™ Flow Cytometry beads for calibration, alignment, counting and instrument set-up to ensure flow and optical stability and for developing assays used for the simultaneous detection and quantitation of up to 24 different...

Our diverse collection of antibodies, assays, beads and buffers support leading research areas. Antibodies NovaFluor dye–conjugated antibodies Brilliant Ultra Violet and Brilliant Violet Antibodies Assays and reagents Beads for compensation, instrument controls, and standards 5 steps to...

The purpose of this learning center is to connect scientists (whether new or experienced) to our many resources for learning about flow cytometry applications, techniques and basic principles by providing a few key points of entry into the vast content.

Flow cytometry provides a rapid method to quantify cell characteristics. However, most flow cytometers cannot directly provide the cell concentration or absolute count of cells in a sample. Absolute cell counts have been widely used in quantifying cell populations and disease progression research,...

Controls, compensation and calibration are all critical to obtaining accurate results in flow cytometric analysis. In this section you will find educational resources including application notes, videos, articles and other useful tools to help you set up your flow cytometer and analyze your samples.

Explore our “Getting Started” and “Troubleshooting” sections for solutions to top inquiries and common problems. Browse through our "Guides and Tools" section to access a comprehensive portfolio of product-related support resources., For Research Use Only. Not for use in diagnostic procedures.

Simultaneously analyze multiple proteins, gene expression, and cell functions such as oxidation, viability, cell cycle, apoptosis, and proliferation from an individual cell with fluorescent flow cytometry assays, reagents, and antibodies.

Spectral flow cytometry is based on many of the fundamental aspects of conventional flow cytometry but has unique optical collection and analytical capabilities. With spectral flow cytometry, the emission spectrum of every fluorescence molecule is captured by a set of detectors across a defined...

Our bacteria counting and enumeration assays for flow cytometry contain a mixture of fluorescent nucleic acid stains for bacterial identification, as well as a calibrated suspension of beads for accurate sample volume measurements.

Flow cytometry methods articles are created to educate and discuss the most current methodologies related to flow cytometry applications, research, and technologies. Navigate the articles and guides below to learn about advances in flow cytometry, best practices, and approaches for correctly...

Learn about assays and techniques for detecting and monitoring cell health and function by flow cytometry. Topics include apoptosis, cell counting, proliferation and viability as well as cell cycle, endocytosis, oxidative stress and more.