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Compensation beads capture species-specific antibodies conjugated to fluorophores and other types of reagents. The purpose of these beads is to set voltages and gating parameters for obtaining accurate fluorescence signal.

(See a list of the products featured in this article.) The discovery in the early 1960s [1] and subsequent development of Green Fluorescent Protein (GFP) as a reporter gene has greatly advanced the study of gene expression, protein localization, and cell and tissue development in a multitude of...

Download compensation and instrument beads flyer, Flow cytometry is used to obtain quantifiable results in reference to the physical characteristics of single cells. Microspheres are small, non-biological beads that can be used as a (1) standard for flow cytometer instrument calibration, (2)...

Ensure optimal flow cytometer instrument performance by using Thermo Scientific™ Flow Cytometry beads for calibration, alignment, counting and instrument set-up to ensure flow and optical stability and for developing assays used for the simultaneous detection and quantitation of up to 24 different...

The ability to stain live cells with a viability dye and preserve that staining pattern after fixation is critical for intracellular immunophenotyping. Exclusion of the dead cells from the data allows cleaner separation and identification of cell populations.

The purpose of this learning center is to connect scientists (whether new or experienced) to our many resources for learning about flow cytometry applications, techniques and basic principles by providing a few key points of entry into the vast content.

Explore our “Getting Started” and “Troubleshooting” sections for solutions to top inquiries and common problems. Browse through our "Guides and Tools" section to access a comprehensive portfolio of product-related support resources., For Research Use Only. Not for use in diagnostic procedures.

Controls, compensation and calibration are all critical to obtaining accurate results in flow cytometric analysis. In this section you will find educational resources including application notes, videos, articles and other useful tools to help you set up your flow cytometer and analyze your samples.

Simultaneously analyze multiple proteins, gene expression, and cell functions such as oxidation, viability, cell cycle, apoptosis, and proliferation from an individual cell with fluorescent flow cytometry assays, reagents, and antibodies.

Go to Panel Builder Build Panels with an Expert Create a new immunophenotyping experiment or add antibodies and reagents to an older panel Insert antibodies already used in your experiment View fluorochrome spectrums to quantify emission spectral overlap Export an excel document with your antibody...

Flow cytometry provides a rapid method to quantify cell characteristics. However, most flow cytometers cannot directly provide the cell concentration or absolute count of cells in a sample. Absolute cell counts have been widely used in quantifying cell populations and disease progression, including...

Spectral flow cytometry is based on many of the fundamental aspects of conventional flow cytometry but has unique optical collection and analytical capabilities. With spectral flow cytometry, the emission spectrum of every fluorescence molecule is captured by a set of detectors across a defined...

Learn about assays and techniques for detecting and monitoring cell health and function by flow cytometry. Topics include apoptosis, cell counting, proliferation and viability as well as cell cycle, endocytosis, oxidative stress and more.

Our bacteria counting and enumeration assays for flow cytometry contain a mixture of fluorescent nucleic acid stains for bacterial identification, as well as a calibrated suspension of beads for accurate sample volume measurements.

Assess key metabolic processes and monitor bacteria/yeast growth, proliferation, and vitality, including the LIVE/DEAD BacLight Bacterial Viability and Counting Kit. See related products, Colored and fluorescent particles Phagocytosis kits and particles Microbial detection and identification kits...