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Our Hypoxia Green Reagent for Flow Cytometry is already highly fluorescent before using the product. What could have caused this? Product FAQ

Answer

This could be due to storage of the product under anaerobic or low oxygen conditions. The Hypoxia Green Reagent for Flow Cytometry increases in fluorescence upon exposure to low oxygen environments, and this change is not reversible. For some ROS indicators, we recommend storing the reagent under dry nitrogen or argon to prevent oxidation during storage. However, this is not appropriate for the Hypoxia Green Reagent for Flow Cytometry.

Answer Id:: E16813

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What is the approximate excitation and emission peaks of the CellTrace Far Red Proliferation reagent? Product FAQ

Answer

The approximate excitation and emission peaks of this product after hydrolysis are 630 nm and 661 nm, respectively. Cells labeled with CellTrace Far Red reagent can be visualized by fluorescence microscopy using standard Cy 5 filter sets or analyzed by flow cytometry in an instrument equipped with a 633/635 nm excitation source and APC channel.

Answer Id:: E9784

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Flow cytometry: an improved method for the selection of highly productive gene-amplified CHO cells using flow cytometry. Citations & References

  • Authors: Yoshikawa T, Nakanishi F, Ogura Y, Oi D, Omasa T, Katakura Y, Kishimoto M, Suga KI
  • Journal: Biotechnol Bioeng
  • PubMed ID: 11427945
Catalog # M1198MP

Can I fix my CellEvent Caspase 3/7 - labeled cells? Product FAQ

Answer

For imaging applications, the CellEvent product signal may be retained after fixation in 3.7% formaldehdye for 15 min. Fixation is not recommended for flow cytometry analysis.

Answer Id:: E14994

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Flow cytometry-based apoptosis detection. Citations & References

  • Authors: Wlodkowic D, Skommer J, Darzynkiewicz Z,
  • Journal: Methods Mol Biol
  • PubMed ID: 19609746

Detection and quantitation of cell-cell electrofusion products by flow cytometry. Citations & References

  • Authors: Jaroszeski MJ, Gilbert R, Heller R
  • Journal: Anal Biochem
  • PubMed ID: 7513970
Catalog #

What are the advantages of flow cytometry? Product FAQ

Answer

-Measures data from single cells.
-Data are obtained for a large number of cells, generating a rich statistical analysis of cell populations.
-Because single cells are measured, it will reveal heterogeneity within a population.
-With the ability to multiplex, small sub-populations can be identified.
-Thousands of cells can be analyzed rapidly.
-It is ideally suited for blood samples and other cells in suspension.
-Data can be re-analyzed multiple times after acquisition.
-Flow cytometry files (FCS) can be archived.

Answer Id:: E14798

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Applications and perspectives of multi-parameter flow cytometry to microbial biofuels production processes. Citations & References

  • Authors: da Silva TL, Roseiro JC, Reis A,
  • Journal: Trends Biotechnol
  • PubMed ID: 22257766

An evaluation of the effects of cytokines on intracellular oxidative production in normal neutrophils by flow cytometry. Citations & References

  • Authors: Yuan L, Inoue S, Saito Y, Nakajima O
  • Journal: Exp Cell Res
  • PubMed ID: 8262156
Catalog # D399

Flow cytometry and sorting in Arabidopsis. Citations & References

  • Authors: Galbraith DW,
  • Journal: Methods Mol Biol
  • PubMed ID: 24057384

What determines whether positive or negative isolation should be used with Dynabeads magnetic beads? Product FAQ

Answer

When cell purity is the most important criteria, target cell activation is not a concern, or when the downstream applications are isolation of RNA or gDNA, then positive isolation is recommended. When the most important criteria is to isolate cells with minimum disturbance, and where the downstream applications include cell culture, study cell function, morphology, and flow cytometry, then negative isolation is recommended (note that positive isolation of cells can also be used when the downstream applications include cell culture, flow cytometry, or studying cell function, but then a positive isolation with bead release from the cells are required, by using e.g., FlowComp products or the Positive Isolation Kits containing DETACHaBEAD products).

Answer Id:: E12093

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Rapid production of quasi-stable antibody-phycoerythrin conjugates for use in flow cytometry. Citations & References

  • Authors: Dale GL
  • Journal: Cytometry
  • PubMed ID: 9845444
Catalog #

What is a suitable generic antibody starting concentration for Western blotting (WB), ELISA, immunohistochemistry (IHC), immunofluorescence (IF), immunoprecipitation (IP), or flow cytometry (FC)? Product FAQ

Answer

It is recommended that you first check the product insert for any recommended usage guidelines.

For general purposes, starting concentrations are as follows:
ELISA--0.5-1.0 µg/mL
WB--1-5 µg/mL
IP--2-5 µg per reaction (1 X 10e6 cells)
IHC/IF--1-10 µg/mL
FC--10-20 µg/mL

Please note that these are general guidelines only.

Answer Id:: E4942

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Simultaneous measurement by flow cytometry of phagocytosis and hydrogen peroxide production of neutrophils in whole blood. Citations & References

  • Authors: Hasui M, Hirabayashi Y, Kobayashi Y
  • Journal: J Immunol Methods
  • PubMed ID: 2913161
Catalog #

Detection of intracellular cytokines by flow cytometry. Citations & References

  • Authors: Jung T, Schauer U, Heusser C, Neumann C, Rieger C
  • Journal: J Immunol Methods
  • PubMed ID: 8445253
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