Citations & References

Fluorescent dyes as probes to study lipid-binding proteins.

  • Authors: Pastukhov AV, Ropson IJ
  • Journal: Proteins (2003) 53:607-615
  • PubMed ID: 14579352

Citations & References

Advantages of calcium green-1 over other fluorescent dyes in measuring cytosolic calcium in platelets.

  • Authors: Lee SK, Lee JY, Lee MY, Chung SM, Chung JH
  • Journal: Anal Biochem (1999) 273:186-191
  • PubMed ID: 10469489

Citations & References

Characterization of calcium translocation across the plasma membrane of primary osteoblasts using a lipophilic calcium-sensitive fluorescent dye, calcium green C18.

  • Authors: Lloyd QP, Kuhn MA, Gay CV
  • Journal: J Biol Chem (1995) 270:22445-22451
  • PubMed ID: 7673232

Citations & References

Use of fluorescent dyes as molecular probes for the study of multidrug resistance.

  • Authors: Neyfakh AA
  • Journal: Exp Cell Res (1988) 174:168-176
  • PubMed ID: 3335222
Catalog #

Citations & References

Appraising the suitability of succinimidyl and lipophilic fluorescent dyes to track proliferation in non-quiescent cells by dye dilution.

  • Authors: Filby A, Begum J, Jalal M, Day W,
  • Journal: Methods (2015) 82:29-37
  • PubMed ID: 25802116

Product FAQ

My fluorescent dye signal is fading as I image it. What can I do to stop this?

Answer

All fluorescent dyes will fade, or “photobleach”, to at least some extent when exposed to strong light at the wavelengths they absorb. Here are some causes for photobleaching and ways to fix the problem:

1) Cause of photobleacing - Generation of free radicals and singlet oxygen
Remedy - i) Use an antifade reagent, which has antioxidants and free radical scavengers:

ii) For live-cell imaging of fluorescent dyes and proteins, we recommend ProLong Live Antifade Reagent which can be added to the cell media or buffer. ProLong Live Antifade Reagent can significantly increase the stability over time for reagents as well as fluorescent proteins, like GFP, without affecting cell health, for up to 24 hours.

iii) For immediate analysis and short-term storage of fixed samples, we recommend SlowFade Diamond Antifade Mountant (it is a liquid mountant and can be used for immediate viewing and then disposal of the sample within a day).

iv) For long-term analysis of Alexa Fluor dyes in fixed samples, we recommend a mountant that hardens, such as ProLong Diamond Antifade Mountant. The harrdening of the mountant also slows diffusion of free radicals).

v) For long-term analysis of all dyes and fluorescent proteins in fixed samples, we recommend ProLong Diamond Antifade Mountant, suitable for archiving slides.

2) Cause of photobleaching - Dye is particularly sensitive to structural modification upon exposure to light.
Remedy - i) Choose a more photostable dye, such as many of our Alexa Fluor dyes.

3) Cause of photobleaching - Intense Illlumination
Remedy - i) Reduce light exposure, for example by reducing laser power or using neutral density filters.

ii) Minimize the viewing time of labeled sample, and close shutter when not viewing.

iii) Use an objective with a lower numerical aperture, such as a lower-power objective.

You can find more information on choosing an antifade reagent on the link below http://www.thermofisher.com/us/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/mounting-medium-antifades.html.

Answer Id: E14792

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Citations & References

Caged Fluorescent Dye Based Studies of Turbulent Scalar Mixing.

  • Authors: Guilkey J, et al.
  • Journal: Proc SPIE-Int Soc Opt Eng (1995) 2546:160-160
Catalog #

Citations & References

Use of fluorescent dye BCECF to measure intracellular pH in cortical collecting tubule.

  • Authors: Weiner ID, Hamm LL
  • Journal: Am J Physiol (1989) 256:F957-F964
  • PubMed ID: 2719125

Citations & References

Imaging of interferences between cellular particles and the fluorescent dye Merocyanine-540 during mormoxia and anoxia.

  • Authors: Mahlke C, Dammann M, Steinbach V, Stingl M-T, Kessler M
  • Journal: Proc SPIE-Int Soc Opt Eng (2002) 4623:346-357
Catalog #
  • M24571(Discontinued)

Citations & References

Membrane trafficking in pituitary cells: studies using a fluorescent dye.

  • Authors: Stafford SJ, Schofield JG
  • Journal: Biochem Soc Trans 1993; (21 ( Pt 3)):3 320S-320S
  • PubMed ID: 8224465

Citations & References

Merocyanine 540 - A Fluorescent Dye and a Biological Probe.

  • Authors: Parkanyi C, Adenier A, Aaron J-J
  • Journal: NA (1996) 0:286-286
Catalog #
  • M24571(Discontinued)
  • M299

Citations & References

Interactions of nucleic acids with fluorescent dyes: spectral properties of condensed complexes.

  • Authors: Kapuscinski J
  • Journal: J Histochem Cytochem (1990) 38:1323-1329
  • PubMed ID: 1696951
Catalog #

Citations & References

Mechanisms of DNA uptake by mammalian cells: fate of exogenously added DNA monitored by the use of fluorescent dyes.

  • Authors: Loyter A, Scangos GA, Ruddle FH
  • Journal: Proc Natl Acad Sci U S A (1982) 79:422-426
  • PubMed ID: 6952193

Citations & References

The Cellular Pathway of Photosynthate Transfer in the Developing Wheat Grain. I. Delineation of a Potential Transfer Pathway Using Fluorescent Dyes.

  • Authors: Wang HL, et al.
  • Journal: Plant Cell Environ (1994) 17:257-257
Catalog #
  • C1359
  • C1360
  • C195
  • H348
  • C1361(Discontinued)
  • C1362(Discontinued)
  • C1904(Discontinued)
  • C194(Discontinued)
  • S6957(Discontinued)
  • C7507