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Fluorescence photooxidation with eosin: a method for high resolution immunolocalization and in situ hybridization detection for light and electron microscopy. Citations & References

  • Authors: Deerinck TJ, Martone ME, Lev-Ram V, Green DP, Tsien RY, Spector DL, Huang S, Ellisman MH
  • Journal: J Cell Biol (1994) 126:901-910
  • PubMed ID: 7519623
Catalog #
  • E18(Discontinued)
  • S6372

Optimization of biotinyl-tyramide-based in situ hybridization for sensitive background-free applications on formalin-fixed, paraffin-embedded tissue specimens. Citations & References

  • Authors: Evans MF, Aliesky HA, Cooper K
  • Journal: BMC Clin Pathol (2003) 3:2-2
  • PubMed ID: 12801424
Catalog # T24831(Blocked)

Could you provide an overview of the ULYSIS Nucleic Acid Labeling Kits? Product FAQ

Answer

The ULYSIS oligonucleotide chemical labeling reagents allow you to rapidly and easily couple our fluorescent dyes to purine bases in nucleic acid polymers. The method, the Universal Linkage System (ULS), is based on the use of a platinum coordination complex that forms a stable adduct at the N7 position of guanine. ULYSIS labeled probes are used in fluorescence in situ hybridization (FISH) and numerous other applications.

Answer Id:: E8090

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What cellular processes can be analyzed with a flow cytometer? Product FAQ

Answer

-Calcium flux: Each of the Oregon Green calcium indicators binds intracellular calcium with increasing affinity, providing a sensitivity range to match many applications. Oregon Green probes emit green fluorescence at resting levels of Ca2+ and increase their fluorescence intensity 14-fold with increasing Ca2+ concentration. The cell-permeant formulation (Cat. No. O6807) can be loaded in cell media and is compatible with flow cytometry.
-Rhodamine-based calcium indicators comprise a range of probes for large or small changes in Ca2+ concentration. They exhibit a 50-fold increase in fluorescence upon calcium binding and offer a range of wavelengths that can be used in conjunction with GFP or green-fluorescent dyes for multiplexing. Rhod-2, AM (Cat. No. R1245MP), in particular, localizes to mitochondria and can be used with flow cytometry.
-Membrane potential: A distinctive feature of the early stages of apoptosis is the disruption of the mitochondria, including changes in membrane and redox potential. We offer a range of products specifically designed to assay mitochondrial membrane potential in live cells by flow cytometry, with minimal disruption of cellular function. The MitoProbe family of mitochondrial stains (Cat. Nos. M34150, M34151, and M34152) provide quick, easy, and reliable flow cytometric detection of the loss of mitochondrial membrane potential that occurs during apoptosis. MitoTracker dyes (Cat. Nos. M7510 and M7512) are membrane potential-dependent probes for staining mitochondria in live cells. The staining pattern of MitoTracker dyes is retained throughout subsequent flow cytometry immunocytochemistry, DNA end labeling, in situ hybridization, or counterstaining steps. The Mitochondrial Permeability Transition Pore Assay (Cat. No. M34153) provides a more direct method of measuring mitochondrial permeability transition pore opening than assays relying on mitochondrial membrane potential alone. The mitochondrial permeability transition pore (MPTP) is a non-specific channel formed by components from the inner and outer mitochondrial membranes, and appears to be involved in the release of mitochondrial components during cell death.
-Phagocytosis: In phagocytosis, cells internalize particulate matter such as microorganisms, and this process is important for immune responses and during the clearance of apoptotic cells. Probes for studying phagocytosis include BioParticles indicators—bacteria and yeast labeled with fluorescent dyes.
-Tracking phagocytosis using a quench/wash-based assay can report on simple uptake, or a pH indicator can be used to monitor stages in the pathway. We have no-wash assays labeled with pHrodo Red or Green (Cat. Nos. A10010, P35361, P35364, P35365, P35366, and P35367) and no-wash assays for whole blood (Cat. Nos. A10025, A10026, P35381, and P35382), all suitable for flow cytometry.
-pH changes: Sensitive pH determinations can be made in a physiological range using either fluorescent intensity or ratiometric measurements. pHrodo dyes (Cat. Nos. P35373 and P35372) provide signal intensity modulation from pH 2 to pH 9 and with a choice of fluorescent wavelengths. Tracking internalization of fluorescent dextran is a routine method for analyzing pH changes in cellular compartments. Dextran conjugates of pHrodo dyes (Cat. Nos. P35368 and P10361) provide the most complete solution by allowing discrimination of vesicles from early endosomes to lysosomes, with no quench or wash required.
-Reactive oxygen species: Cells that are environmentally stressed usually contain greatly increased levels of reactive oxygen species (ROS). CellROX reagents are fluorogenic probes developed for the detection and quantitation of ROS in live cells. These cell-permeant reagents are non-fluorescent or very weakly fluorescent in the reduced state; however, when oxidized, they become brightly fluorescent and remain localized within the cell. We offer CellROX Green (Cat. No. C10492), CellROX Orange (Cat. No. C10493), and CellROX Deep Red (Cat. No. C10491) Assay Kits validated for flow cytometry.

Answer Id:: E14827

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Product Brochure: Plate Guide Product Literature

Laboratory Product Checklist - Protein, RNA, cell, and DNA Product Literature

Phase II study of erlotinib in recurrent or metastatic endometrial cancer: NCIC IND-148 Citations & References

  • Authors: Oza, AM; Eisenhauer, EA; Elit, L; Cutz, JC; Sakurada, A; Tsao, MS; Hoskins, PJ; Biagi, J; Ghatage, P; Mazurka, J; Provencher, D; Dore, N; Dancey, J; Fyles, A
  • Journal: JOURNAL OF CLINICAL ONCOLOGY 2008 26:4319-4325

Abundance and diversity of Archaea in heavy-metal-contaminated soils. Citations & References

  • Authors: Sandaa RA; Enger O; Torsvik V
  • Journal: Applied and Environmental Microbiology 1999 8:3293-3297
Catalog #
  • 4318739(Discontinued)
  • 4327058(Discontinued)
  • 4327059(Discontinued)

Brochure: Immunoassay Guide to Protein Quantitation Product Literature

Several types of mutations of the Abl gene can be found in chronic myeloid leukemia patients resistant to STI571, and they can pre-exist to the onset of treatment. Citations & References

  • Authors: Roche-Lestienne C; Soenen-Cornu V; Grardel-Duflos N; Lai JL; Philippe N; Facon T; Fenaux P; Preudhomme C
  • Journal: Blood 2002 3:1014-1018
Catalog #
  • 4318739(Discontinued)
  • 4327058(Discontinued)
  • 4327059(Discontinued)

User Guide: ViewRNA ISH Tissue Assay Manual / Product Insert

  • Version: D.0
Catalog #

Early differences in fecal microbiota composition in children may predict overweight. Citations & References

  • Authors: Kalliomaki M; Collado MC; Salminen S; Isolauri E
  • Journal: The American Journal of Clinical Nutrition 2008 3:534-538

User Guide: MitoTracker Mitochondrion-Selective Probes Manual / Product Insert

  • Version: 06-25-2008
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