Freeze-dried tendon Allografts as tissue-engineering scaffolds for gdf5 gene delivery Citations & References
- Authors: Basile, P; Dadali, T; Jacobson, J; Hasslund, S; Ulrich-Vinther, M; Soballe, K; Nishio, Y; Drissi, MH; Langstein, HN; Mitten, DJ; O'Keefe, RJ; Schwarz, EM; Awad, HA
- Journal: MOLECULAR THERAPY
Characterization of a reference material for BCR-ABL (M-BCR) mRNA quantitation by real-time amplification assays: towards new standards for gene expression measurements. Citations & References
- Authors: Saldanha J; Silvy M; Beaufils N; Arlinghaus R; Barbany G; Branford S; Cayuela JM; Cazzaniga G; Gonzalez M; Grimwade D; Kairisto V; Miyamura K; Lawler M; Lion T; Macintyre E; Mahon FX; Muller MC; Ostergaard M; Pfeifer H; Saglio G; Sawyers C; Spinelli O; va
- Journal: Leukemia : Official Journal of the Leukemia Society of America, Leukemia Research Fund, U.k
[Tetrazolium methods for the histochemical investigation of hydrolases (author's transl)] Citations & References
Remodeling of cortical bone allografts mediated by adherent rAAV-RANKL and VEGF gene therapy. Citations & References
- Authors: Ito H; Koefoed M; Tiyapatanaputi P; Gromov K; Goater JJ; Carmouche J; Zhang X; Rubery PT; Rabinowitz J; Samulski RJ; Nakamura T; Soballe K; O'Keefe RJ; Boyce BF; Schwarz EM
- Journal: Nature Medicine
Probiotic lactobacilli in a semi-soft cheese survive in the simulated human gastrointestinal tract Citations & References
Improvement of periodontal condition by probiotics with Lactobacillus salivarius WB21: a randomized, double-blind, placebo-controlled study Citations & References
- Authors: Shimauchi, H; Mayanagi, G; Nakaya, S; Minamibuchi, M; Ito, Y; Yamaki, K; Hirata, H
- Journal: JOURNAL OF CLINICAL PERIODONTOLOGY
Hepatitis C virus RNA in dried serum spotted onto filter paper is stable at room temperature. Citations & References
- Authors: Abe K; Konomi N
- Journal: Journal of Clinical Microbiology
Altered hyperlipidemia, hepatic steatosis, and hepatic peroxisome proliferator-activated receptors in rats with intake of tart cherry Citations & References
- Authors: Seymour, EM; Singer, AAM; Kirakosyan, A; Urcuyo-Llanes, DE; Kaufman, PB; Bolling, SF
- Journal: JOURNAL OF MEDICINAL FOOD
Histidine decarboxylases and their role in accumulation of histamine in tuna and dried saury. Citations & References
- Authors: Kanki M; Yoda T; Tsukamoto T; Baba E
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How should I resuspend my siRNA library? Product FAQ
RNA oligonucleotides are susceptible to degradation by exogenous ribonucleases introduced during handling. Wear gloves when handling this product. Use RNase-free reagents, tubes, and barrier pipette tips. Upon receipt, your siRNAs may be safely stored in a non-frost-free freezer at or below -20 degrees C (dried oligonucleotides are shipped at ambient temperature). Invitrogen siRNA reagents are shipped as dry pellets at ambient temperature and should be stored at -20 degrees C upon arrival in a manual defrost or noncycling freezer. Under these conditions, the siRNAs are stable for at least 3 years. If necessary siRNAs as dry pellets (unopened) can be stored at 4 degrees C for at least a year.
To resuspend Invitrogen siRNAs provided in plates:
Centrifuge each plate at low speed (maximum RCF 4,000 x g) to collect the contents at the bottom of the wells before removing the seal.
Wipe the adhesive foil cover with 70% ethanol or other RNase-decontamination solution such as RNaseZap RNase Decontamination Solution (Cat. No. AM9780, AM9782, AM9784).
1. Thermo Scientific or carefully peel back the foil seal to gain access to wells. Use caution and avoid shredding the seal.
2. Add nuclease-free, sterile water, using a multichannel pipettor or liquid handling system and sterile tips, to achieve the desired concentration. Resuspend siRNAs to a convenient stock concentration using the recommended volume of Invitrogen Nuclease-Free Water (not DEPC-treated). Concentrated stocks of 10 µM or more are recommended. However, stock solutions of 2-5 µM may better accommodate dilution schemes for high?throughput transfections and assays conducted on robotic platforms.
3. Gently pipet up and down 5 times to resuspend. Place the solution on an orbital mixer/shaker for 70-90 minutes at room temperature. This additional mixing results in more reliable resuspension.
4. Centrifuge briefly to collect the liquid at the bottom of the wells, if necessary.
5. (Optional) Aliquot the siRNAs into one or more daughter plates, to limit the number of freeze/thaw cycles to which the siRNAs are subjected.
6. Store at -20 degrees C or lower for long-term storage. The siRNAs can be stored at 4 degrees C for short-term use, but care should be taken to seal well to avoid evaporation.
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