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Catalogue: TopAir Fume Hoods Product Literature

Why does the manual for the MagMAX FFPE DNA/RNA Ultra Kit suggest using Citrisolv Clearing Agent for removal of paraffin from the FFPE samples? Can I use xylene instead? Product FAQ

Answer

Yes, xylene can be used as well. Citrisolv Clearing Agent is a healthier alternative to xylene as it does not need to be handled under a fume hood.

Answer Id: E13116

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Can I fix my gels in ethanol/acetic acid rather than methanol/acetic acid prior to staining with SYPRO Ruby Gel Stain? Product FAQ

Answer

Yes, but ethanol/acetic acid will produce ethyl acetate, which has a strong odor, so you should do this fixation in a fume hood.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Answer Id: E11134

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Specification Sheet: TopAir Metal Fume Hood Product Literature

Specification Sheet: TopAir Polypropylene Fume Hood Product Literature

Why run a glyoxal gel instead of a formaldehyde denaturing agarose gel? Product FAQ

Answer

Using glyoxal/DMSO instead of formaldehyde avoids the need to pour and run gels in a fume hood and eliminates safety issues associated with formaldehyde.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

Answer Id: E7959

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Specification Sheet: TopAir Polypropylene Walk-In Fume Hood Product Literature

Specification Sheet: TopAir Polypropylene Active Fume Hood Product Literature

Specification Sheet: TopAir ductless fume hood: PRO Product Literature

Specification Sheet: TopAir Polypropylene Wet Scrubber Fume Hood Product Literature

Specification Sheet: TopAir ductless fume hood – PRO with Airflow Alarm Product Literature

Specification Sheet: TopAir ductless fume hood – MID with Airflow Alarm Product Literature

Specification Sheet: TopAir Polypropylene Educational Fume Hood with 360° Transparency Product Literature

Brochure: Versatile NMR Spectrometer for Bench Chemistry Product Literature

Is there an alternative to the liquid nitrogen method for lysing of yeast colonies/patches for a filter-lift assay? Product FAQ

Answer

Yes, here is a protocol for Chloroform Lysis:
1. In a ventilation hood, place 3 ml of chloroform on the lid of the plate (agar side up).
2. Let sit 5 mins at room temperature. The chloroform fumes will rise and lyse the cells.
3. Carefully separate the lid from the rest of the plate. The chloroform fumes will damage the plate and make the rim of the lid "gooey"
4. Place the filter over the colonies and proceed with your assay.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Answer Id: E3765

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