Electrophoresis is a common lab technique used to identify, quantify, and purify nucleic acid fragments and assess quality. RNA molecules are negatively charged, and during gel electrophoresis they migrate toward the anode in the presence of an electric current.
Chemistry students Kelsey Holbert and Marie Nider from the University of Wisconsin Milwaukee will be giving presentations at this year's Pittcon, demonstrating online reaction monitoring with our Thermo Scientific™ picoSpin™ spectrometers.
Chemical reactions often involve the use of air- and moisture-sensitive solvents, and pyrophoric or hazardous reagents. Our AcroSeal packaging is a packaging solution designed to enable safe handling of these types of materials which are used in a variety of research and development applications,...
Stripping and reprobing a western blot is a method in which the primary and secondary antibodies are removed from a western blot so the blot can be reprobed. A blot may be stripped and reprobed several times to visualize other proteins or to optimize detection of a protein (i.e.
Over the last 50 years, nuclear magnetic resonance (NMR) spectroscopyhas become the preeminent technique for determining the structure of organic compounds. However, because NMR spectrometers are typically large, expensive machines requiring strong, liquid helium-cooled cooled superconducting...
Twin-screw extrusion is a standard technique for polymer mixing and compounding, but has also expanded into other applications, such as novel materials development. Extruders offer a high level of process flexibility, control of process parameters, and can produce a wide range of products.
Northern blot analysis reveals information about RNA identity, size, and abundance, allowing a deeper understanding of gene expression levels. Our Invitrogen™ portfolio comprises one of the industry’s most comprehensive product offerings for northern blot analysis.
How can I increase the sensitivity of my Northern blots? Is it possible to make the Northern blotting procedure faster? Can Northerns be used to detect small RNAs? Is there a way to effectively strip Northerns? How can I prevent cross hybridization to rRNA sequences?
Researchers who work with RNA know that RNA is a sensitive molecule that gets rapidly degraded. Because of the single-stranded nature of RNA, it is easily accessible to proteins to inactivate and target for degradation.
Properly setting up your HPLC-CAD system is crucial for maximizing the overall detector performance. This page presents best lab practices for installing your HPLC-CAD system, but you should always refer to our operating manuals as the primary source of information!
Optimize your experiments to get the best results. We’ve compiled a detailed knowledge base of the top tips and tricks to meet your research needs., View the relevant questions below:, Browse our FAQ database for more information ›, Need more information? Contact us ›, For Research Use Only.
Northern analysis remains a standard method for detection and quantitation of mRNA levels despite the advent of powerful techniques, such as RT-PCR, gene array analysis and nuclease protection assays. Northern analysis provides a direct relative comparison of message abundance between samples on a...
Optimize your experiments to get the best results. We’ve compiled a detailed knowledgebase of the top tips and tricks to meet your research needs. View the relevant questions below:, Browse our FAQ database for more information ›, Need more information? Contact us ›, For Research Use Only.
For Research Use Only. Not for use in diagnostic procedures., KingFisher systems can handle a broad range of applications across nucleic acids, proteins and peptides, cells and exosomes. Different sample types and applications require different chemistries to extract the analyte.
Find valuable information. Optimize your experiments to get the best results. We’ve compiled a detailed knowledge base of the top tips and tricks to meet your research needs. View the relevant questions below:, Browse our FAQ database for more information ›, Need more information?