Two-dimensional (2D) gel electrophoresis is a powerful and sensitive technique for separating and analyzing protein mixtures from biological samples. 2D gel electrophoresis is performed in two consecutive steps, IEF and SDS-PAGE.
Finding the right protein gel electrophoresis system is critical to getting consistent and accurate results you need for your research. Electrophoresis buffers and reagents are important components of the protein electrophoresis system.
Protein gel electrophoresis is a simple way to separate proteins prior to downstream detection or analysis. Our portfolio of high-quality protein electrophoresis products unites gels, gel tanks, protein gel handcast system, stains, molecular weight markers and standards, running buffers, and...
In biolaboratories, agarose gel electrophoresis is the modus operandi for size-based separation of DNA and RNA fragments. The agarose gel matrix is porous and acts as a sieve through which the nucleic acid molecules migrate.
When preparing samples for reducing gel electrophoresis, any of the following reducing agents may be used: Electrophoresis sample buffers Protein gels NuPAGE Reducing Agent Dithiothreitol (DTT), 50 mM final concentration ϐ-mercaptoethanol, 2.
For Research Use Only. Not for use in diagnostic procedures., View all What are ZOOM™ 2D Protein Solubilizers? Which one should I use? What is the pI performance range for your IEF gels? How should I equilibrate ZOOM™ strips before performing SDS-PAGE using ZOOM™ gels?
Nucleic acid electrophoresis is a standard laboratorytechnique that underpins molecular biology research. Traditionally, gel electrophoresis takes a lot of time and involves casting the gel, making buffers, preparing samples and ladders, loading and running the gel,as well as visualizing and imaging...
Electrophoresis underpins many molecular biology applications. Therefore, problems in nucleic acid gel electrophoresis hinders downstream applications and hampers experimental workflow; often errors in gel electrophoresis negatively impact the results of an experiment.
Gel electrophoresis is a common laboratory technique to isolate nucleic acids and proteins. Nucleic acid electrophoresis uses a gel matrix to separate DNA and RNA fragments based on size and molecular weight. Gel electrophoresis is vital in all molecular biology workflows.
Choosing the right electrophoresis products for your nucleic acid analysis workflow is critical to the success of your experiment.This article is a quick guide on how to improve gel electrophoresis results.
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Optimize your experiments to get the best results. We’ve compiled a detailed knowledgebase of the top tips and tricks to meet your research needs. View the relevant questions below:, Browse our FAQ database for more information ›, Need more information? Contact us ›, For Research Use Only.
We are dedicated to your success. Get back on track. View our expert recommendations for commonly encountered problem scenarios., View the relevant questions below:, Browse our FAQ database for more information ›, Need more information? Contact us ›, For Research Use Only.
Nucleic acid gel electrophoresis is widely used in molecular biology research. Gel electrophoresis is a proven and effective method to separate, resolve, and quantitate nucleic acid molecules. The applications of gel electrophoresis can be grouped as analytical and preparative.