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What are the power supply requirements for the SureLock Tandem Midi Gel Tank? Do you offer a power supply that I can use with it? Product FAQ

Answer

The SureLock Tandem Midi Gel Tank should be used with an external DC power supply designed for electrophoresis. This power supply must be isolated from the ground so that the DC output is floating (safety requirement) and must be equipped with No Load or Open Circuit protection. It must be able to operate at 300 V and have an output current minimum of at least 1 A (1000 mA). We recommend using the PowerEase Touch 350W Power Supply (Cat. No. PS0350) with the SureLock Tandem Midi Gel Tank. Note: To allow connection of the covered power leads of the SureLock Tandem Midi Gel Tank with certain power supplies that are not designed for use with covered or non-retractable power leads, we recommend using Novex Power Supply Adapters available separately (Cat. No. ZA10001).

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Answer Id: E19950

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What are the power supply requirements for the XCell4 SureLock Midi-Cell? Do you offer a power supply that I can use with it? Product FAQ

Answer

The XCell4 SureLock Midi-Cell should be used with an external DC power supply designed for electrophoresis. This power supply must:

- Be isolated from the ground so that the DC output is floating (safety requirement)and must be equipped with No Load or Open Circuit protection
- Be able to operate at 200 V

If you would like to simultaneously run 4 gels using the XCell4 SureLock Midi-Cell, we recommend using the ZOOM Dual Power Supply. The Low Voltage/High Current outlets can handle the voltage and current of 4 NuPAGE Bis-Tris gels (Voltage 200 V, Current ~800 mA). The PowerEase 90W Power Supply and PowerEase 500 Power Supply (discontinued as of December 31, 2013) can be used with the XCell4 SureLock Midi-Cell but the current limit of 500 mA will be reached with 2 gels (2 x ~200 mA max current at 200V). If 4 gels are run in the XCell4 SureLock Midi-Cell with these power supplies, the current will be maxed out and the run will slow down. It will take approximately twice as long to run the gels. Note: To allow connection of the covered power leads of the XCell4 SureLock Midi-Cell with certain power supplies that are not designed for use with covered or non-retractable power leads, use Invitrogen Power Supply Adapters available separately (Cat. No. ZA10001).

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Answer Id: E10945

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I am transferring my gel using constant voltage and the current reading has dropped much lower than the expected starting current. What could have happened? Product FAQ

Answer

Here are possible causes and solutions:

- The buffer was accidentally made too dilute, therefore increasing resistance and thus lowering conductivity and current: Check the transfer buffer and its reagent components and then re-dilute it or remake it.
- The circuit is broken or impeded, as in the case of a corroded or broken electrode or malfunctioning power supply: Check the equipment.
- There is a leak in the blot module (this is indicated by a drastic decrease in current and in buffer volume within the module): Ensure that the inner buffer chamber is filled sufficiently so that the wells are covered with buffer.
- Tape at the bottom of the gel cassette was not removed: Double check that the tape on the bottom of the gel has been removed.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Answer Id: E11597

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Nucleic Acid Detection on Gels, Blots and Arrays—Section 8.4 Molecular Probes Handbook

Protein Detection on Gels, Blots and Arrays—Section 9.3 Molecular Probes Handbook

Protein Quantitation in Solution—Section 9.2 Molecular Probes Handbook

Assays for Apoptosis and Autophagy—Section 15.5 Molecular Probes Handbook

Other Nonpolar and Amphiphilic Probes—Section 13.5 Molecular Probes Handbook

Nucleic Acid Quantitation in Solution—Section 8.3 Molecular Probes Handbook

Nucleic Acid Stains—Section 8.1 Molecular Probes Handbook

Reagents for Analysis of Low Molecular Weight Amines—Section 1.8 Molecular Probes Handbook

Introduction to Amine Modification—Section 1.1 Molecular Probes Handbook

Pre-Cast Gels for Medium/High Throughput Nucleic Acid Analysis Experiment Protocol

Detecting Peptidases and Proteases—Section 10.4 Molecular Probes Handbook

Pre-Cast Gels for Safe Nucleic Acid Analysis Experiment Protocol

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