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MSQ18LA Nitrogen Generator for Mass Spectrometers User Manual Manual / Product Insert

  • Version: FEB.2016

Why is the temperature of UHV chamber higher than expected when using Orbitrap Exactive or Q Exactive mass spectrometry instruments? Product FAQ

Answer

The UHV chamber heating control may have failed. During general operation of the mass spectrometer, the temperature of the UHV chamber is not regulated. Only during a system bakeout, electric power is supplied to the heating elements of the UHV chamber. The heating always operates at maximum power. Thus, failure of the heating control does not lead to a dangerous overheating of the mass spectrometer. If the Exactive Series mass spectrometer does not work as expected, use the Tune software for error diagnosis:

- The messages window displays real-time information about the statuses of the instrument, the control service, or other programs. If the heating was terminated, the window displays a corresponding error message.

In case of a failure of the UHV chamber heating control, shut down the mass spectrometer as described on page 6-8 of the manual (https://assets.thermofisher.com/TFS-Assets/CMD/manuals/man-bre0012255-exactive-series-manbre0012255-en.pdf). To prevent permanent damage to components of the Exactive Series mass spectrometer, Thermo Fisher Scientific recommends that you call a Thermo Fisher Scientific field service engineer.

Answer Id:: E16864

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When using Orbitrap Exactive or Q Exactive mass spectrometry instruments, which resolution settings do I choose for different scan modes for the quantification of small molecules? Product FAQ

Answer

In PRM, you can choose a lower resolution because of the selectivity of MS2 ions.

If you need to do polarity switching in Full Scan mode, it is a good idea to reduce resolution to 35,000 to generate more data points. However, this might generate less data points.

If you want to work qualitatively, you might want to consider to work with lock masses and the highest resolution (fine isotopic pattern). View table here for suggested resolution settings.

Answer Id:: E16857

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When using Orbitrap Exactive or Q Exactive mass spectrometry instruments, how do I find the optimal settings for Automated Gain Control (AGC) and maximum injection time (IT)? Product FAQ

Answer

AGC targets of Full Scan: 1 x 10E6 and SIM/PRM: 2 x 10E5 are good starting points. The more similar the ions that are collected in the C-trap are, the lower the target should be.

Max IT:

View table here to see the scan times required for the different resolution settings.

In the simplest case, set the max IT to times just below these values - parallel acquisition.

In Full Scan mode, usually the defined max IT time is not used by the system as the ion flux is very high. In data dependent MS2 scans, the time can be extended to receive a better spectrum quality. However, this might generate less data points.

Answer Id:: E16856

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Application Note: Structural Determination of Flavonoids Using MSn Product Literature

Novel mass spectrometry-based tool for genotypic identification of mycobacteria. Citations & References

  • Authors: Lefmann M; Honisch C; Bocker S; Storm N; von Wintzingerode F; Schlotelburg C; Moter A; van den Boom D; Gobel UB
  • Journal: Journal of Clinical Microbiology
Catalog #

How much protein is required for mass spectrometry analysis by MALDI-TOF? Product FAQ

Answer

In general, the required amount needed to identify a gel-separated protein by enzymatic digestion (usually Trypsin) is about 2 pmole (equivalent to approximately 0.12 µg). Our SilverQuest Silver staining kit is mass spectrometry compatible.

Answer Id:: E4285

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Huntingtin phosphorylation sites mapped by mass spectrometry. Modulation of cleavage and toxicity. Citations & References

  • Authors: Schilling B, Gafni J, Torcassi C, Cong X, Row RH, LaFevre-Bernt MA, Cusack MP, Ratovitski T, Hirschhorn R, Ross CA, Gibson BW, Ellerby LM
  • Journal: J Biol Chem
  • PubMed ID: 16782707
Catalog #

Chip-based genotyping by mass spectrometry. Citations & References

  • Authors: Tang K, Fu DJ, Julien D, Braun A, Cantor CR, Köster H
  • Journal: Proc Natl Acad Sci U S A
  • PubMed ID: 10468554

Chip-based genotyping by mass spectrometry. Citations & References

  • Authors: Tang K; Fu DJ; Julien D; Braun A; Cantor CR; Koster H
  • Journal: Proceedings of the National Academy of Sciences of the United States of America
Catalog #
  • 4318739(Discontinued)
  • 4327059(Discontinued)
  • 4327058(Discontinued)

Combined liquid chromatography/mass spectrometry of the radical adducts of a fluorescamine-derivatized nitroxide. Citations & References

  • Authors: Johnson CG, Caron S, Blough NV
  • Journal: Anal Chem
  • PubMed ID: 8779444
Catalog # C7924(Discontinued)

Advantages of Thermococcus kodakaraenis (KOD) DNA Polymerase for PCR-mass spectrometry based analyses. Citations & References

  • Authors: Benson LM; Null AP; Muddiman DC
  • Journal: Journal of the American Society for Mass Spectrometry
Catalog #

Combining avidin-biotin chemistry with matrix-assisted laser desorption/ionization mass spectrometry. Citations & References

  • Authors: Schriemer DC, Li L
  • Journal: Anal Chem
  • PubMed ID: 8843136
Catalog #
  • B1513(Discontinued)
  • B6353(Discontinued)

A sialylation study of mouse brain gangliosides by MALDI a-TOF and o-TOF mass spectrometry Citations & References

  • Authors: Zarei, M; Bindila, L; Souady, J; Dreisewerd, K; Berkenkamp, S; Muething, J; Peter-Katalinic, J
  • Journal: JOURNAL OF MASS SPECTROMETRY

Studies on 1-alkylamine adduct formation in electrospray ionization mass spectrometry for quantitative analysis Citations & References

  • Authors: Teshima, K; Yoneyama, T; Kondo, T
  • Journal: JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
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