Manual / Product Insert

Human/Monkey Extracellular Protein Buffer Reagent for Magnetic Kits

Version: Rev 1.00 (9 April 2012)
Catalog #

Product FAQ

What type of applications can the Versette Automated Liquid Handler support?

Answer

The Versette Automated Liquid Handler can perform a variety of flexible applications, not limited to the following:

- Cell culture/Cell therapy
- Compound transfers
- ELISA
- Food and beverage
- Genomics
- Mass spectrometric immunoassay (MSIA)
- PCR/qPCR
- Proteomics
- Vitamin D assay
- Other general liquid handling needs

Answer Id: E17166

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Product Literature

Drug Monitoring, Quality Controls and Instrumentation Catalog 2018 [EN]

Product FAQ

How do I develop a sandwich ELISA using Cytosets?

Answer

Each CytoSets contains capture (coating) antibody, biotinlyated detection antibody, standard and Streptavidin-HRP. Other reagents required are listed in the CytoSets information sheet included with the kit and can be purshased from us separately (Antibody Pair Buffer kit CNB0011, 5x Assay Buffer DS98200, etc.). The information sheet also provides a specific procedure and illustrates an example standard curve which can be obtained when the specific procedure is followed. A general procedure is summarized here:

1) Coat the microplate with diluted capture (coating) antibody overnight at 2-8 degrees C; Wash the plate
2) Incubate standards or samples with the coated microplate; Wash the plate
3) Incubate diluted biotinlyated detection antibody with the plate; Wash the plate
4) Incubate Streptavidin-HRP with the plate for 15-45 minutes; Wash the plate
5) Incubate the plate with TMB substrate for 10-60 minutes and stop the reaction with Stop solution
6) Read microplate at 450 nm.

Investigators are advised to determine optimal buffer formulations, concentrations and incubation times for individual applications.

Answer Id: E5138

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Product FAQ

How do I process tissue homogenates for use on the Luminex assay platform?

Answer

We recommend following the protocol provided below, which was developed using the Tissue Extraction Reagent I (Cat. No. FNN0071), and shows good correlation between ELISA and Luminex technology. This procedure has been applied to multiple tissue types. However, we recommend that you optimize for each tissue sample type used. Similar extraction reagents/lysis buffers may be used.

Add protease inhibitors to the Tissue Extraction Reagent just before use.
Weigh the tissue sample.
Add 10 mL of the Tissue Extraction Reagent per 1 gram of tissue.
Homogenize the tissue.
Centrifuge the sample at 10,000 rpm for 5 min to pellet the tissue debris.
Collect the supernatant. Follow the assay procedure provided with the kit for appropriate dilutions; this is to prevent/minimize potential inhibition of antibody-antigen binding by the detergent present in the extraction or lysis buffer. In general, tissue homogenates or cell lysates (depending on the lysis buffer used) need to be diluted 5- to 10-fold to reduce the detergent concentration to ≤0.01%. However individual kits/samples may require further dilution with assay diluent or standard diluent based on the concentration of the cytokine/protein of interest.
Note: If the samples are to be stored, aliquot them and freeze at -80 degrees C. Avoid multiple freeze-thaw cycles.

Answer Id: E12643

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Manual / Product Insert

Technical Data Sheet: Mouse IFN-gamma Antibody Pair, Lot 1721366

Version: MAN0014791 Rev. 1.0 (13Jan2016)
Catalog #
  • CMC4033(Discontinued)

Manual / Product Insert

User Guide: Human sIL-2R ELISA Kit

Version: Jan. 2015
Catalog #
  • EH2IL2R2(Discontinued)
  • EH2IL2R5(Discontinued)

Product Literature

Application Note: Beverages Applications Notebook: Milk

Manual / Product Insert

User Guide: Mouse MCP-1 ELISA Reagent Kit

Version: Jan. 2015

Product Literature

Application Note: Food Safety Applications Notebook: Adulteration

Manual / Product Insert

Fluoroskan Ascent FL

Manual / Product Insert

Protocol: Thermo Scientific™ Human IL-2 ELISA Kit

Version: MAN0014639 Rev. 2 (5Sep2017)

Manual / Product Insert

Product Sheet: ProQuantum Human EPO Immunoassay Kit

Version: A.0
Catalog #

Manual / Product Insert

IDEIA Herpes Simplex Virus [ES]

Version: FEB.2016

Product Literature

Life in the Lab - Stay Curious (South Asia)