Product FAQ

Why is the glutamine requirement so high for Express Five® media?

Answer

The glutamine concentration is correct. Insect media have historically been 2 to 10 fold higher in glutamine concentration vs. classical mammalian media. Express Five SFM was optimized for High Five cells which have especially high requirements for glutamine and asparagine. The liquid basal media is actually 1.09x in concentration and was designed to be diluted to the correct 1x composition with 90 ml/L of the 200 ml L-glutamine. Because the L-glutamine concentration is so high, we left it out of the basal formulation to avoid problems associated with glutamine degradation and the resulting ammonia build-up which can be toxic.

Answer Id: E3050

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Product FAQ

Why did my 200 mM L-Glutamine precipitate out of solution when I thawed it?

Answer

When L-glutamine is in a concentrated stock solution it easily precipitates when cooled. Warming the solution briefly in a 37C water bath with gentle swirling will dissolve the precipitate. Do not use the product unless the precipitate is fully dissolved.

Answer Id: E4500

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Product FAQ

Will the media formulations containing GlutaMAX supplement change with respect to L-glutamine content?

Answer

In all media containing GlutaMAX supplement dipeptides as a substitute for L-glutamine, concentration is equimolar with the L-glutamine in the original formulation.

Answer Id: E2984

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Product FAQ

There is a precipitate in my Penicillin-Streptomycin-Glutamine solution upon thaw, is this still good to use?

Answer

Yes, this is still good to use. The precipitate is due to the glutamine. The solution needs to be warmed to room temp and swirled unitl the glutamine goes back into solution.

Answer Id: E13285

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Product FAQ

What is the recommended concentration of Penicillin-Streptomycin-Glutamine to use?

Answer

The Penicillin-Streptomycin-Glutamine solution is at 100X. This can be used at a 1X concentration in media.

Answer Id: E13286

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Product FAQ

For Penicillin-Streptomycin-Glutamine, what is the concentration of each component?

Answer

This contains: Penicillin G Sodium at 12,000 Units/mL, Streptomycin Sulfate at 10,000 mcg/mL, L-glutamine at 29.2 mg/mL (200 mM), and Sodium Citrate 0.14% NaCl at 10mM

Answer Id: E13283

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Product FAQ

What is the difference between Gibco GlutaMAX and glutamine supplements?

Answer

GlutaMAX-I supplement is an improved cell culture supplement that can be used as a direct substitute for L-glutamine in your cell culture medium. Please go here to read more about the properties of GlutaMAX supplement (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/media-supplements/glutamax-media/glutamax-vs-glutamine.html).

Answer Id: E11884

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Product FAQ

Can I add GlutaMAX to Express Five SFM instead of L-Glutamine?

Answer

We recommend that you use L-Glutamine instead of GlutaMAX, as insect cells lack the ability to sufficiently cleave the dipeptide bond in the GlutaMax, making it a poor source of glutamine for these cells.

Answer Id: E17471

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Product FAQ

Can GlutaMAX be used instead of Glutamine to supplement CTS OpTmizer T-Cell Expansion SFM?

Answer

GlutaMAX cannot replace Glutamine because cells may not initially use GlutaMAX so that T cells cannot grow. We recommend adding 4 mM GlutaMAX and 2 mM Glutamine (as stated in the manual) into the CTS OpTmizer T-Cell Expansion SFM if GlutaMAX is needed.

Answer Id: E16615

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Product FAQ

Will depletion, absence, or breakdown of essential growth-promoting components such as glutamine or growth factors reduce the growth rate of my culture?

Answer

Yes. If you suspect that this is the case, remove the medium and add fresh medium. Alternatively, you can supplement medium with growth-promoting components. It is also possible to substitute GlutaMax I or II for glutamine in the medium to prevent glutamine exhaustion.

Answer Id: E11919

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Product FAQ

What is the stability of L-Glutamine- and serum-supplemented complete media when stored at 4 degrees C?

Answer

We recommend that complete media containing L-Glutamine and serum be used within 4 weeks of making it when stored at 2-8 degrees C. Unlike most other amino acids, L-Glutamine is labile in solution and breaks down into ammonia, which is harmful to cells. The rate at which this degradation proceeds is a function of time, temperature, and pH.

Answer Id: E17454

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Product FAQ

Will the formulations containing Gibco GlutaMAX supplement change with respect to L-glutamine content?

Answer

In all media containing GlutaMAX supplement dipeptides as a substitute for L-glutamine, concentration is equimolar with the L-glutamine in the original formulation.

Answer Id: E11918

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Product FAQ

What is the stability of L-Glutamine-supplemented media when stored at 4 degrees C?

Answer

L-Glutamine-supplemented media are stable for 12 months when stored at 2 - 8 degrees C. Unlike most other amino acids, L-Glutamine is labile in solution and breaks down into ammonia which is harmful to cells. The rate at which this degradation proceeds is a function of time, temperature, and pH.

Answer Id: E17469

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