Product FAQ

In the catalog, it is said that Hanks' Balanced Salt solutions are intended for use in ambient air, not in CO2 equilibrated incubator.What is the reason for that? Also, what is the functional difference between Hanks' Balanced Salt Solutions and Earle's Balanced Salt Solutions?

Answer

The basic difference between Earle's salts and Hanks' salts is the level of sodium bicarbonate. It is much higher in Earle's (2.2g/L) than in Hanks' (0.35g/L). Sodium bicarbonate balances with high levels of CO2 (5%) to hold the pH of a solution. If Earle's salts are left in ambient levels of CO2 a solution will become alkaline whereas a Hanks' buffered solution in a CO2 incubator will become acidic. If you wish to store tissue in the CO2 incubator, you need to use Earle's. However if you are simply rinsing tissue that will be stored later in cell culture medium, Hanks' will be fine.

Answer Id: E3170

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Product FAQ

Is a precipitate in HBSS (with calcium and magnesium) normal?

Answer

No, this is not normal and if there is a precipitate in the product, it cannot be used. If the precipitate will not go back into solution, please contact Technical Support at techsupport@thermofisher.com for a replacement.

Answer Id: E13268

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Product FAQ

Why do you offer HBSS with and without phenol red?

Answer

Phenol red is a pH indicator which allows you to determine the optimal atmospheric conditions for your cell line during your particular assays. We offer both versions to allow you the option to utilize this indicator in your media.

Answer Id: E13270

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Product FAQ

Can I use HBSS (with calcium and magnesium) to wash my cells before dissociation?

Answer

No, a saline solution that contains calcium and magnesium should not be used to wash cells before dissociation.

Answer Id: E13269

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Product FAQ

Do I need to add additional HEPES to Hank's Balanced Salt Solution (HBSS) (Cat. No. 14025xxx)?

Answer

HBSS (Cat. No. 14025xxx) does not contain HEPES and it needs to be externally supplemented. The complete formulation is available here (https://www.thermofisher.com/us/en/home/technical-resources/media-formulation.153.html).

Answer Id: E17448

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Product FAQ

Can I store Hank's Balanced Salt Solution (HBSS) in the refrigerator?

Answer

The recommended storage condition for our HBSS products is at 15-30 degrees C. Generally, storage at 4 degrees C will not impact these products. The only concern would be for the 10x HBSS solutions with calcium and magnesium as these salts might precipitate when stored outside the recommended temperature ranges. As long as you don't see any precipitation upon refrigeration, the product will be fine.

Answer Id: E17458

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Media Formulation

15210 - KaryoMAX(R) Colcemid Solution in HBSS

Catalog #
    15210057(Discontinued)

Product FAQ

I’m using an Amplex Red kit, the reagent changes color to pink almost immediately in my own Krebs-Ringer buffer but not in HBSS. Why is this?

Answer

The components of Krebs-Ringer buffer (salts) should not cause oxidation of the Amplex reagent (which, in the presence of peroxidase and H2O2 oxidizes to resorufin, which is pink in color and fluorescent). Try water alone (the water used to make the Krebs-Ringer buffer). Since Hank’s Buffered Saline Solution is typically purchased rather than made in the lab, it likely would not have the same contaminant. Another option is to degas the buffer prior to use to removed dissolved oxygen radicals.

Answer Id: E6502

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Media Formulation

21575 - MEM, Hank's

Catalog #

Media Formulation

11012 - MEM, Hank's, powder

Catalog #

Media Formulation

14170 - HBSS, no calcium, no magnesium

Catalog #