Immunoassay Kit Grade


Product Line


Target Species Validated


Validated Application

Products

IFN gamma Monoclonal Antibody (GZ-4), FITC, eBioscience™ Invitrogen™

IFN gamma Monoclonal Antibody for Flow

IFN gamma Monoclonal Antibody (4S.B3), Biotin, eBioscience™ Invitrogen™

IFN gamma Monoclonal Antibody for ELISA

IFN gamma Monoclonal Antibody (4S.B3), eFluor 506, eBioscience™ Invitrogen™

IFN gamma Monoclonal Antibody for Flow

IFN gamma Monoclonal Antibody (XMG1.2), Alexa Fluor 488, eBioscience™ Invitrogen™

IFN gamma Monoclonal Antibody for Flow

IFN-α A Pure Recombinant Mouse Protein Gibco™

Recombinant Mouse Interferon α A (IFN-αA) is a bioactive protein intended for use in cell culture applications. IFN-α is involved in antiviral and antiparasitic activities.

High purity—no interference from other proteins or contaminants
High biological activity—more results with less protein
Proven compatibility—Gibco® proteins bioassayed with Gibco® media

High Purity Recombinant Bioactive Protein
Recombinant Mouse Interferon α A is one of our more than 250 available Gibco® recombinant proteins. To help ensure Gibco® growth factors are of the highest quality, each protein is analyzed for purity along with structural homogeneity to ensure a biologically active protein.

High Biological Activity
In-house activity testing of Gibco® Recombinant Proteins includes cell proliferation, cytotoxicity, chemotaxis, calcium flux, secondary cytokine up-regulation, induction of surface antigen expression, antiviral, and protease assays. Recombinant Mouse Interferon α A (IFN-αA) is specific activity is determined by titration with the use of the cytopathic effect inhibition assay.

Proven Compatibility
Gibco® proteins are bioassayed with Gibco® media. Since 1962, Gibco® has been the consistent provider of high quality media, reagents, and sera for reliable cell culture.

See our selection of recombinant Gibco® growth factors.

For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.

IFN gamma Porcine ProcartaPlex™ Simplex Kit Invitrogen™

The Porcine IFN gamma Simplex ProcartaPlex Kit measures IFN gamma protein and is designed to be combinable with other Simplex kits so that you can create your own multiplex panel that utilizes Luminex xMAP technology for protein detection/quantitation. When combining multiple Simplex kits (i.e., when you are not using a pre-configured Multiplex Panel), only one buffer kit (sold separately) is needed for each assay plate regardless of plex size.

About ProcartaPlex Assays for the Luminex Platform

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate all protein targets simultaneously using a Luminex instrument. ProcartaPlex multiplex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

More results per sample—measure multiple protein targets simultaneously in a single 25–50 µL sample

Well-established Luminex technology—highly referenced multiplexing platform for protein detection and quantitation

ProcartaPlex assays utilize Luminex xMAP (multianalyte profiling) technology for the simultaneous detection and quantitation of up to 65 protein targets in a single 25–50 µL sample from plasma, serum, cell culture supernatants, and other bodily fluids.

The Luminex beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create spectrally unique signatures that can be identified by the Luminex xMAP detection systems (e.g. Luminex 200, FLEXMAP 3D, and MAGPIX). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a multiplexed assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex multiplex panels are available in multiple formats across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information, including a comprehensive list of individual protein targets.

Mouse IFN gamma Recombinant Protein, eBioscience™ Invitrogen™

Mouse IFN gamma Recombinant Protein for Ctrl

IL-29 (IFN lambda 1) Human ProcartaPlex™ Simplex Kit Invitrogen™

The Human IL-29 (IFN lambda 1) Simplex ProcartaPlex Kit measures IL-29 (IFN lambda 1) protein and is designed to be combinable with other Simplex kits so that you can create your own multiplex panel that utilizes Luminex xMAP technology for protein detection/quantitation. When combining multiple Simplex kits (i.e., when you are not using a pre-configured Multiplex Panel), only one buffer kit (sold separately) is needed for each assay plate regardless of plex size.

About ProcartaPlex Assays for the Luminex Platform

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate all protein targets simultaneously using a Luminex instrument. ProcartaPlex multiplex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

More results per sample—measure multiple protein targets simultaneously in a single 25–50 µL sample

Well-established Luminex technology—highly referenced multiplexing platform for protein detection and quantitation

ProcartaPlex assays utilize Luminex xMAP (multianalyte profiling) technology for the simultaneous detection and quantitation of up to 65 protein targets in a single 25–50 µL sample from plasma, serum, cell culture supernatants, and other bodily fluids.

The Luminex beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create spectrally unique signatures that can be identified by the Luminex xMAP detection systems (e.g. Luminex 200, FLEXMAP 3D, and MAGPIX). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a multiplexed assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex multiplex panels are available in multiple formats across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information, including a comprehensive list of individual protein targets.

Mouse IFN alpha 2 Recombinant Protein, eBioscience™ Invitrogen™

Mouse IFN alpha 2 Recombinant Protein for Ctrl, FN

IFN gamma Monoclonal Antibody (XMG1.2), FITC, eBioscience™ Invitrogen™

IFN gamma Monoclonal Antibody for Flow

IFN gamma Monoclonal Antibody (MD-1), eBioscience™ Invitrogen™

IFN gamma Monoclonal Antibody for Western Blot, IHC (F), Flow, ELISA

IFN gamma Monoclonal Antibody (4S.B3), Alexa Fluor 488, eBioscience™ Invitrogen™

IFN gamma Monoclonal Antibody for Flow

IFN-γ Recombinant Mouse Protein Gibco™

Recombinant Mouse Interferon-γ (IFN-γ) is a bioactive protein intended for use in cell culture applications. IFN-γ is involved in many biological processes such as inducing the secretion of TNF-α and stimulating the release of reactive oxygen species from macrophages. It is also involved with bone growth and inhibits bone resorption. It is antiviral and antiparasitic and inhibits the proliferation of cells.

High purity—no interference from other proteins or contaminants
High biological activity—more results with less protein
Proven compatibility—Gibco® proteins bioassayed with Gibco® media

High Purity Recombinant Bioactive Protein
Recombinant Mouse Interferon-γ is one of our more than 250 available Gibco® recombinant proteins. To help ensure Gibco® growth factors are of the highest quality, each protein is analyzed for purity along with structural homogeneity to ensure a biologically active protein.

High Biological Activity
In-house activity testing of Gibco® Recombinant Proteins includes cell proliferation, cytotoxicity, chemotaxis, calcium flux, secondary cytokine up-regulation, induction of surface antigen expression, antiviral, and protease assays. Recombinant Mouse Interferon-γ (IFN-γ) specific activity is determined in a dose response cytostasis assay of the mouse cell line WeHi279, as measured by the reduction of MTS.

Proven Compatibility
Gibco® proteins are bioassayed with Gibco® media. Since 1962, Gibco® has been the consistent provider of high quality media, reagents, and sera for reliable cell culture.

See our selection of recombinant Gibco® growth factors.

For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.

IFN-β Recombinant Rat Protein Gibco™

Recombinant IFN-β is a bioacitve protein intended for use in cell culture applications. IFN-β is involved in immune responses against viral infection.

IFN gamma Human ELISA Kit, High Sensitivity Invitrogen™

IFN gamma Human ELISA Kit, High Sensitivity for ELISA
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