Sort By

IFNB1 (INF-beta) Recombinant Human Protein, hIgG1-Fc Tag

Interferon, beta 1 (IFNB1), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in performing cell based assays. It can also be used as a protein standard, or in other research applications.

This recombinant protein was expressed from a DNA sequence encoding the human IFNB1 (NP_002167.1), residues 1 to 187, fused to the Fc region of human IgG1 at the C-terminus.

Activity: Measured in antiviral assays using WISH human amnion cells infected with vesicular stomatitis virus (VSV). The ED50 for this effect is 0.5-2 ng/ml.

Formulation: Lyophilized in PBS, pH 7.4, 5% Mannitol, 5% Trehalose, and 0.02% Tween 80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.



This protein is manufactured by Sino Biological Inc.

IFN-β Recombinant Rat Protein (Gibco™)

Recombinant IFN-β is a bioacitve protein intended for use in cell culture applications. IFN-β is involved in immune responses against viral infection.

IFN beta Human ELISA Kit (Invitrogen™)

IFN beta Human ELISA Kit for ELISA

IFN-beta Human ProcartaPlex™ Simplex Kit (Invitrogen™)

The Human IFN-beta Simplex ProcartaPlex kit measures IFN-beta protein and is designed to be combinable with other Simplex kits so that you can create your own multiplex panel that utilizes Luminex xMAP technology for protein detection/quantitation. When combining multiple Simplex kits (i.e., when you are not using a pre-configured Multiplex Panel), only one buffer kit (sold separately) is needed for each assay plate regardless of plex size.

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate up to 80 protein targets simultaneously when using the FLEXMAP 3D or Luminex 200 instrument and up to 50 protein targets when using the MAGPIX instrument. ProcartaPlex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

Flexible panels—design your own panels with Simplex kits to measure your own array of targets
More results per sample—measure up to 80 protein targets in a single 25–50 µL sample
Well-established Luminex technology—the most referenced multiplexing platform for protein detection and quantitation

The Luminex MagPlex superparamagnetic microsphere beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create 100 spectrally unique signatures that can be identified by the Luminex xMAP detec¬tion systems (Luminex 200, FLEXMAP 3D, and MAGPIX systems). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a ProcartaPlex multiplex assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a magnetic bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile up to 80 times more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex Simplex kits provide the ability to create your own unique panel . More than 90% of ProcartaPlex Simplex targets can be combined, providing you with superior flexibility when creating your own multiplex panel.

ProcartaPlex Simplex kits are available across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information, including a comprehensive list of individual protein targets.

Reactivity/Species
Human

Suitable Sample Types
cell culture supernatant, serum, plasma

Sample Volume
Serum, Plasma: 25 µL; CCS: 50 µL

Reported Application
Multiplex Immunoassay

IFN-β1A Mammalian Recombinant Human Protein (Gibco™)

Recombinant IFN-β is a bioacitve protein intended for use in cell culture applications. IFN-β is involved in immune responses against viral infection.

IFN beta Mouse ELISA Kit (Invitrogen™)

IFN beta Mouse ELISA Kit for ELISA

IFN-beta Mouse ProcartaPlex™ Simplex Kit (Invitrogen™)

The Mouse IFN-beta Simplex ProcartaPlex kit measures IFN-beta protein and is designed to be combinable with other Simplex kits so that you can create your own multiplex panel that utilizes Luminex xMAP technology for protein detection/quantitation. When combining multiple Simplex kits (i.e., when you are not using a pre-configured Multiplex Panel), only one buffer kit (sold separately) is needed for each assay plate regardless of plex size.

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate up to 80 protein targets simultaneously when using the FLEXMAP 3D or Luminex 200 instrument and up to 50 protein targets when using the MAGPIX instrument. ProcartaPlex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

Flexible panels—design your own panels with Simplex kits to measure your own array of targets
More results per sample—measure up to 80 protein targets in a single 25–50 µL sample
Well-established Luminex technology—the most referenced multiplexing platform for protein detection and quantitation

The Luminex MagPlex superparamagnetic microsphere beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create 100 spectrally unique signatures that can be identified by the Luminex xMAP detec¬tion systems (Luminex 200, FLEXMAP 3D, and MAGPIX systems). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a ProcartaPlex multiplex assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a magnetic bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile up to 80 times more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex Simplex kits provide the ability to create your own unique panel . More than 90% of ProcartaPlex Simplex targets can be combined, providing you with superior flexibility when creating your own multiplex panel.

ProcartaPlex Simplex kits are available across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information, including a comprehensive list of individual protein targets.

Reactivity/species: mouse
Suitable sample types: cell culture supernatant, serum, plasma
Sample volume: Serum, plasma: 25 µL; CCS: 50 µL
Reported application: multiplex immunoassay
Target: IFN-beta
Bead regions: IFN-beta: 77

IFN beta Polyclonal Antibody (Invitrogen™)

IFN beta Polyclonal Antibody for Western Blot, IF, ICC, IHC

IFN beta Polyclonal Antibody (Invitrogen™)

IFN beta Polyclonal Antibody for Western Blot, IHC, IP, ELISA, Neu

IFN beta Polyclonal Antibody (Invitrogen™)

IFN beta Polyclonal Antibody for Neu

IFN beta Polyclonal Antibody (Invitrogen™)

IFN beta Polyclonal Antibody for Neu, Ctrl, FN

IFN beta Monoclonal Antibody (MMHB-1) (Invitrogen™)

IFN beta Monoclonal Antibody for ELISA

Rat IFN beta Recombinant Protein (Invitrogen™)

Rat IFN beta Recombinant Protein for Western Blot, IP, ELISA

IFN beta Polyclonal Antibody (Invitrogen™)

IFN beta Polyclonal Antibody for Neu

IFN beta Polyclonal Antibody (Invitrogen™)

IFN beta Polyclonal Antibody for Western Blot, ELISA, Neu