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Lab Vision™ IGF-1R (α -Subunit) Ab-1, Mouse Monoclonal Antibody (Thermo Scientific™)

Provide accurate, reproducible results with the Thermo Scientific™ IGF-1R (α-Subunit) Ab-1, Mouse Monoclonal Antibody.

Lab Vision™ IGF-1R (α -Subunit) Ab-4, Mouse Monoclonal Antibody (Thermo Scientific™)

Ideal for immunoprecipitation, Thermo Scientific™ IGF-1R (α-Subunit) Ab-4, Mouse Monoclonal Antibody provides accurate, reproducible results.

LanthaScreen™ IGF-1R GripTite™ Cell Line

The IGF-1R⁄PI3K⁄AKT pathway mediates signals for cell growth, cell survival, transcription, translation, and glucose uptake. IGF-1R is a receptor tyrosine kinase (RTK) that resides at the top of the pathway. Upon growth factor binding extracellularly, this RTK undergoes auto-phosphorylation at multiple intracellular tyrosine residues (resulting in pathway activation). The LanthaScreen® IGF-1R GripTite™ (HEK 293 MSR) cellular assay utilizes a human cell line that constitutively expresses IGF-1R fusion proteins with yellow fluorescent protein (YFP). This kinase target was introduced using lipid transfection and these cells are a clonal population isolated by FACS, using YFP fluorescence as a sorting marker and Blasticidin to maintain cells under selection. Using this cell line, a homogenous immunoassay was developed with a time-resolved FRET (TR-FRET) readout in which the insulin-induced phosphorylation of multiple tyrosine residues on IGF-1R is detected in cell lysates using a generic terbium-labeled phosphospecific antibody (Tb-anti-pY20). This cell line has been validated with different stimuli⁄inhibitors and shows correct EC50 ⁄ IC50 values. Moreover, this assay has been optimized for performance under variable experimental conditions (including cell plating density, agonist stimulation time, DMSO tolerance and assay development time) and displays excellent statistical data (Z' > 0.6) and good signal-to-background.

TaqMan™ Array, Human IGF-1R Signaling, Fast 96-well (Applied Biosystems™)

These 96-well plates are pre-configured with the most appropriate TaqMan® Gene Expression Assays for a specific biological process, pathway, or disease state. Each plate contains predefined assays and endogenous controls dried-down in the wells, ready for accurate assessment of an entire gene signature in one simple experiment. The TaqMan® Array Human IGF1R Signaling 96-well Plate contains 92 assays to genes associated with IGF1R signaling and 4 assays to candidate endogenous control genes.

IGF1 Recombinant Rabbit Monoclonal Antibody (23) (Invitrogen™)

IGF1 Recombinant Monoclonal Antibody for ELISA

IGF1 Monoclonal Antibody (126002) (Invitrogen™)

IGF1 Monoclonal Antibody for Western Blot, ELISA, Neu

IGF1 Receptor Recombinant Protein (Invitrogen™)

Human IGF1R beta Recombinant Protein for Ctrl

IGF1 Monoclonal Antibody (56408) (Invitrogen™)

IGF1 Monoclonal Antibody for Western Blot, ELISA

IGF1 Monoclonal Antibody (OTI3A6), TrueMAB™ (OriGene)

IGF1 Monoclonal Antibody for Western blot, IHC (P)

IGF1 Monoclonal Antibody (OTI4B12), TrueMAB™ (OriGene)

IGF1 Monoclonal Antibody for Western blot, IHC (P)

IGF1 Monoclonal Antibody (OTI3A6), TrueMAB™ (OriGene)

IGF1 Monoclonal Antibody for Western blot, IHC (P)

IGF1 Monoclonal Antibody (OTI3A5), TrueMAB™ (OriGene)

IGF1 Monoclonal Antibody for Western blot

IGF1 Monoclonal Antibody (OTI4B12), TrueMAB™ (OriGene)

IGF1 Monoclonal Antibody for Western blot, IHC (P)

IGF1 Monoclonal Antibody (OTI3A5), TrueMAB™ (OriGene)

IGF1 Monoclonal Antibody for Western blot

IGF1 Polyclonal Antibody (Invitrogen™)

IGF1 Polyclonal Antibody for Western Blot, ELISA