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IL-12/IL-23 p40 (Total) Mouse Uncoated ELISA Kit with Plates (Invitrogen™)

IL-12/IL-23 p40 (Total) Mouse Uncoated ELISA Kit with Plates for ELISA

IL-12 p40 Porcine ProcartaPlex™ Simplex Kit (Invitrogen™)

The Porcine IL-12 p40 Simplex ProcartaPlex kit measures IL-12 p40 protein and is designed to be combinable with other Simplex kits so that you can create your own multiplex panel that utilizes Luminex xMAP technology for protein detection/quantitation. When combining multiple Simplex kits (i.e., when you are not using a pre-configured Multiplex Panel), only one buffer kit (sold separately) is needed for each assay plate regardless of plex size.

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate up to 80 protein targets simultaneously when using the FLEXMAP 3D or Luminex 200 instrument and up to 50 protein targets when using the MAGPIX instrument. ProcartaPlex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

Flexible panels—design your own panels with Simplex kits to measure your own array of targets
More results per sample—measure up to 80 protein targets in a single 25–50 µL sample
Well-established Luminex technology—the most referenced multiplexing platform for protein detection and quantitation

The Luminex MagPlex superparamagnetic microsphere beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create 100 spectrally unique signatures that can be identified by the Luminex xMAP detec¬tion systems (Luminex 200, FLEXMAP 3D, and MAGPIX systems). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a ProcartaPlex multiplex assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a magnetic bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile up to 80 times more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex Simplex kits provide the ability to create your own unique panel . More than 90% of ProcartaPlex Simplex targets can be combined, providing you with superior flexibility when creating your own multiplex panel.

ProcartaPlex Simplex kits are available across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information, including a comprehensive list of individual protein targets.

Reactivity/Species
Pig

Suitable Sample Types
cell culture supernatant, serum, plasma

Sample Volume
Serum, Plasma: 25 µL; CCS: 50 µL

Reported Application
Multiplex Immunoassay

Human IL-12 p70 ELISA Standard Recombinant Protein, eBioscience™ (Invitrogen™)

Human IL-12 p70 ELISA Standard Recombinant Protein for ELISA, Ctrl

Mouse IL-12 p40 monomer ELISA Standard Recombinant Protein, eBioscience™ (Invitrogen™)

Mouse IL-12 p40 monomer ELISA Standard Recombinant Protein for ELISA, Ctrl

IL-12 p40 Human ELISA Kit (Invitrogen™)

IL-12 p40 Human ELISA Kit for ELISA

IL-12 p40 Rat ProcartaPlex™ Simplex Kit (Invitrogen™)

The Rat IL-12 p40 Simplex ProcartaPlex kit measures IL-12 p40 protein and is designed to be combinable with other Simplex kits so that you can create your own multiplex panel that utilizes Luminex xMAP technology for protein detection/quantitation. When combining multiple Simplex kits (i.e., when you are not using a pre-configured Multiplex Panel), only one buffer kit (sold separately) is needed for each assay plate regardless of plex size.

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate up to 80 protein targets simultaneously when using the FLEXMAP 3D or Luminex 200 instrument and up to 50 protein targets when using the MAGPIX instrument. ProcartaPlex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

Flexible panels—design your own panels with Simplex kits to measure your own array of targets
More results per sample—measure up to 80 protein targets in a single 25–50 µL sample
Well-established Luminex technology—the most referenced multiplexing platform for protein detection and quantitation

The Luminex MagPlex superparamagnetic microsphere beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create 100 spectrally unique signatures that can be identified by the Luminex xMAP detec¬tion systems (Luminex 200, FLEXMAP 3D, and MAGPIX systems). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a ProcartaPlex multiplex assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a magnetic bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile up to 80 times more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex Simplex kits provide the ability to create your own unique panel . More than 90% of ProcartaPlex Simplex targets can be combined, providing you with superior flexibility when creating your own multiplex panel.

ProcartaPlex Simplex kits are available across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information, including a comprehensive list of individual protein targets.

Reactivity/Species
Rat

Suitable Sample Types
cell culture supernatant, serum, plasma

Sample Volume
Serum, Plasma: 25 µL; CCS: 50 µL

Reported Application
Multiplex Immunoassay

Cytokine & Chemokine Convenience 34-Plex Human ProcartaPlex™ Panel 1A (Invitrogen™)

ProcartaPlex™ immunoassays are our ideal solution for assessing multiple protein biomarkers in a single sample. Our ProcartaPlex assays use Luminex ™ xMAP technology for the multi-analyte detection of secreted proteins including cytokines, chemokines, growth factors and other proteins.
ProcartaPlex™ assays are subject to the same stringent and robust development and manufacturing as are our extensively validated Platinum and Instant ELISA kits. Quantitate multiple targets in cell/tissue culture supernatants, serum, and plasma.

Explore our broad portfolio of pre-configurated panels and combinable single analytes.

NOTE: This kit cannot be combined with other ProcartaPlex Simplex or Multiplex Kits.

Reactivity/Species
Human

Suitable Sample Types
cell culture supernatant, serum, plasma

Sample Volume
Serum, Plasma: 25 µL; CCS: 50 µL

Reported Application
Multiplex Immunoassay

Target List
Eotaxin/CCL11; GM-CSF; GRO alpha/CXCL1; IFN alpha; IFN gamma; IL-1 beta; IL-1 alpha; IL-1RA; IL-2; IL-4; IL-5; IL-6; IL-7; IL-8/CXCL8; IL-9; IL-10; IL-12 p70; IL-13; IL-15; IL-17A; IL-18; IL-21; IL-22; IL-23; IL-27; IL-31; IP-10/CXCL10; MCP-1/CCL2; MIP-1 alpha/CCL3; MIP-1 beta/CCL4; RANTES/CCL5; SDF1 alpha/CXCL12; TNF alpha; TNF beta/LTA

Bead Regions
See product documentation

Assay Range
See product documentation

Assay Sensitivity
See product documentation

Th1/Th2 Cytokine 18-Plex Human ProcartaPlex™ Panel 1C (Invitrogen™)

The Human Th1/Th2 Cytokine IC 18-plex ProcartaPlex Panel is a preconfigured multiplex immunoassay kit that measures 18 protein targets using Luminex xMAP technology. ProcartaPlex preconfigured panels are supplied with all the necessary reagents to perform the assay and are validated to ensure reproducibility and consistency. These panels are designed for those who need greater validation for longitudinal studies.

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate all protein targets simultaneously using a Luminex instrument. ProcartaPlex multiplex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

Reproducible, reliable results—validated as a panel to the highest industry standard, including protein target combinability and cross-reactivity testing
More results per sample—measure multiple protein targets simultaneously in a single 25–50 µL sample
Well-established Luminex technology—the most referenced multiplexing platform for protein detection and quantitation

ProcartaPlex assays utilize Luminex xMAP (multianalyte profiling) technology for the simultaneous detection and quantitation of up to 80 protein targets in a single 25–50 µL sample—from plasma, serum, cell culture supernatants, and other bodily fluids.

The Luminex MagPlex superparamagnetic microsphere beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create 100 spectrally unique signatures that can be identified by the Luminex xMAP detec¬tion systems (Luminex 200, FLEXMAP 3D, and MAGPIX systems). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a multiplexed assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a magnetic bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex multiplex panels are available in multiple formats across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information and available products.

Reactivity/Species
Human

Suitable Sample Types
cell culture supernatant, serum, plasma

Sample Volume
Serum, Plasma: 25 µL; CCS: 50 µL

Reported Application
Multiplex Immunoassay

Target List
GM-CSF; IFN gamma; IL-1 beta; IL-2; IL-4; IL-5; IL-6; IL-12 p70; IL-13; IL-18; TNF alpha; IFN alpha; IL-1 alpha; IL-1RA; IL-7; IL-15; IL-31; TNF beta

Bead Regions
IFN-gamma: 43; IL-12p70: 34; IL-13: 35; IL-1 beta: 18; IL-2: 19; IL-4: 20; IL-5: 21; IL-6: 25; TNF-alpha: 45; GM-CSF: 44; IL-18: 66; IFN-alpha: 48; IL-31: 37; IL-15: 65; IL-1 alpha: 62; IL-1RA:38; IL-7: 26; TNF-beta: 54

Assay Range
[As determined for Lot 1:] IFN gamma: 12.21-50000 pg/mL; IL-12p70: 6.84-28000 pg/mL; IL-13: 2.44-10000 pg/mL; IL-1 beta: 2.44-10000 pg/mL; IL-2: 4.88-20000 pg/mL; IL-4: 12.21-50000 pg/mL; IL-5: 7.32-30000 pg/mL; IL-6: 9.77-40000 pg/mL; TNF-alpha: 8.54-35000 pg/mL; GM-SCF: 17.09-70000; IL-18: 9.77-40000 pg/mL; IFN alpha: 0.61-2500 pg/mL; IL-15: 3.05-12500 pg/mL; IL-1 alpha: 0.61-2500 pg/mL; IL-1RA: 34.18-140000 pg/mL; IL-7: 0.61-2500 pg/mL; TNF-beta: 6.10-25000 pg/mL; IL-31: 19.53-80000 pg/mL

Assay Sensitivity
[As determined for Lot 1:] IFN gamma: 0.2 pg/mL; IL-12p70: 0.04 pg/mL; IL-13: 0.1 pg/ml; IL-1 beta: 0.2 pg/mL; IL-2: 0.8 pg/mL; IL-4: 1.5 pg/mL; IL-5: 0.3 pg/mL; IL-6: 0.4 pg/mL; TNF alpha: 0.4 pg/mL; GM-CSF: 1.2 pg/mL; IL-18: 0.4 pg/mL; IFN alpha: 0.2 pg/mL; IL-15: 1.1 pg/mL; IL-1 alpha: 0.1 pg/mL; IL-1RA: 17.8 pg/mL; IL-7: 0.2 pg/mL; TNF-beta: 1.6 pg/mL; IL-31: 3.3 pg/mL

Cytokine & Chemokine Convenience 36-Plex Mouse ProcartaPlex™ Panel 1A (Invitrogen™)

ProcartaPlex™ immunoassays are our ideal solution for assessing multiple protein biomarkers in a single sample. Our ProcartaPlex assays use Luminex ™ xMAP technology for the multi-analyte detection of secreted proteins including cytokines, chemokines, growth factors and other proteins.
ProcartaPlex™ assays are subject to the same stringent and robust development and manufacturing as are our extensively validated Platinum and Instant ELISA kits. Quantitate multiple targets in cell/tissue culture supernatants, serum, and plasma.

Explore our broad portfolio of pre-configurated panels and combinable single analytes.

NOTE: This kit cannot be combined with other ProcartaPlex Simplex or Multiplex Kits.

Reactivity/Species
Mouse

Suitable Sample Types
cell culture supernatant, serum, plasma

Sample Volume
Serum, Plasma: 25 µL; CCS: 50 µL

Reported Application
Multiplex Immunoassay

Target List
IFN gamma; IL-12p70; IL-13; IL-1 beta; IL-2; IL-4; IL-5; IL-6; TNF alpha; GM-CSF; IL-18; IL-10; IL-17A; IL-22; IL-23; IL-27; IL-9; GRO alpha; IP-10; MCP-1; MCP-3; MIP-1 alpha; MIP-1 beta; MIP-2; RANTES; Eotaxin; IFN alpha; IL-15/IL-15R; IL-28; IL-31; IL-1 alpha; IL-3; G-CSF; LIF; ENA-78/CXCL5; M-CSF

Bead Regions
See product documentation

Assay Range
See product documentation

Assay Sensitivity
See product documentation

Th Complete 14-Plex Rat ProcartaPlex™ Panel (Invitrogen™)

The Rat Th Complete 14-plex ProcartaPlex Panel is a preconfigured multiplex immunoassay kit that measures 14 protein targets using Luminex xMAP technology. ProcartaPlex preconfigured panels are supplied with all the necessary reagents to perform the assay and are validated to ensure reproducibility and consistency. These panels are designed for those who need greater validation for longitudinal studies.

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate all protein targets simultaneously using a Luminex instrument. ProcartaPlex multiplex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

Reproducible, reliable results—validated as a panel to the highest industry standard, including protein target combinability and cross-reactivity testing
More results per sample—measure multiple protein targets simultaneously in a single 25–50 µL sample
Well-established Luminex technology—the most referenced multiplexing platform for protein detection and quantitation

ProcartaPlex assays utilize Luminex xMAP (multianalyte profiling) technology for the simultaneous detection and quantitation of up to 80 protein targets in a single 25–50 µL sample—from plasma, serum, cell culture supernatants, and other bodily fluids.

The Luminex MagPlex superparamagnetic microsphere beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create 100 spectrally unique signatures that can be identified by the Luminex xMAP detec¬tion systems (Luminex 200, FLEXMAP 3D, and MAGPIX systems). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a multiplexed assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a magnetic bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex multiplex panels are available in multiple formats across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information and available products.

Reactivity/Species
Rat

Suitable Sample Types
cell culture supernatant, serum, plasma

Sample Volume
Serum, Plasma: 25 µL; CCS: 50 µL

Reported Application
Multiplex Immunoassay

Target List
IL-1 alpha; G-CSF; IL-10; IL-17A; IL-1 beta; IL-6; TNF alpha; IL-4; GM-CSF; IFN gamma; IL-2; IL-5; IL-13; IL-12p70

Bead Regions
IL-1 alpha: 14; C-CSF (CSF-3): 12; IL-10: 15; IL-17A: 53 ; IL-1 beta: 19; IL-6: 18 ; TNF alpha: 45; IL-4: 21; GM-CSF: 42; IFN gamma: 25; IL-2: 20; IL-5: 27; IL-13: 35; IL-12p70: 39

Assay Range
[As determined for Lot 1:] IL-1 alpha: 12.2-50000 pg/mL; C-CSF (CSF-3): 3.7-15000 pg/mL; IL-10: 9.8-40000 pg/mL; IL-17A: 3.7-15000 pg/mL; IL-1 beta: 15.9-65000 pg/mL; IL-6: 4.9-20000 pg/mL; TNF alpha: 4.9-20000 pg/mL; IL-4: 1.2-5000 pg/mL; GM-CSF: 9.8-40000 pg/mL; IFN gamma: 9.8-40000 pg/mL; IL-2: 4.9-20000 pg/mL; IL-5: 2.4-10000 pg/mL; IL-13: 4.9-20000 pg/mL; IL-12p70: 9.8-40000 pg/mL

Assay Sensitivity
[As determined for Lot 1:] IL-1 alpha: 3.5 pg/mL; C-CSF (CSF-3): 0.3 pg/mL; IL-10: 1.6 pg/mL; IL-17A: 0.2 pg/mL; IL-1 beta: 1.1 pg/mL; IL-6: 1.9 pg/mL; TNF alpha: 0.4 pg/mL; IL-4: 0.1 pg/mL; GM-CSF: 1.3 pg/mL; IFN gamma: 0.6 pg/mL; IL-2: 1.9 pg/mL; IL-5: 0.3 pg/mL; IL-13: 0.3 pg/mL; IL-12p70: 5.9 pg/mL

Cytokine & Chemokine 22-Plex Rat ProcartaPlex™ Panel (Invitrogen™)

The Rat Cytokine & Chemokine 22-plex ProcartaPlex Panel is a preconfigured multiplex immunoassay kit that measures 22 protein targets using Luminex xMAP technology. ProcartaPlex preconfigured panels are supplied with all the necessary reagents to perform the assay and are validated to ensure reproducibility and consistency. These panels are designed for those who need greater validation for longitudinal studies.

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate all protein targets simultaneously using a Luminex instrument. ProcartaPlex multiplex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

Reproducible, reliable results—validated as a panel to the highest industry standard, including protein target combinability and cross-reactivity testing
More results per sample—measure multiple protein targets simultaneously in a single 25–50 µL sample
Well-established Luminex technology—the most referenced multiplexing platform for protein detection and quantitation

ProcartaPlex assays utilize Luminex xMAP (multianalyte profiling) technology for the simultaneous detection and quantitation of up to 80 protein targets in a single 25–50 µL sample—from plasma, serum, cell culture supernatants, and other bodily fluids.

The Luminex MagPlex superparamagnetic microsphere beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create 100 spectrally unique signatures that can be identified by the Luminex xMAP detec¬tion systems (Luminex 200, FLEXMAP 3D, and MAGPIX systems). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a multiplexed assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a magnetic bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex multiplex panels are available in multiple formats across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information and available products.

Reactivity/Species
Rat

Suitable Sample Types
cell culture supernatant, serum, plasma

Sample Volume
Serum, Plasma: 25 µL; CCS: 50 µL

Reported Application
Multiplex Immunoassay

Target List
IL-1 alpha; G-CSF; IL-10; IL-17A; IL-1 beta; IL-6; TNF alpha; IL-4; GM-CSF; IFN gamma; IL-2; IL-5; IL-13; IL-12p70; Eotaxin; GRO alpha; IP-10; MCP-1; MCP-3; MIP-1 alpha; MIP-2; Rantes

Bead Regions
See product documentation

Assay Range
[As determined for Lot 1:] IL-1 alpha: 12.2-50000 pg/mL; C-CSF (CSF-3): 3.7-15000 pg/mL; IL-10: 9.8-40000 pg/mL; IL-17A: 3.7-15000 pg/mL; IL-1 beta: 15.9-65000 pg/mL; IL-6: 4.9-20000 pg/mL; TNF alpha: 4.9-20000 pg/mL; IL-4: 1.2-5000 pg/mL; GM-CSF: 9.8-40000 pg/mL; IFN gamma: 9.8-40000 pg/mL; IL-2: 4.9-20000 pg/mL; IL-5: 2.4-10000 pg/mL; IL-13: 4.9-20000 pg/mL; IL-12p70: 9.8-40000 pg/mL; Eotaxin: 1.9-7700 pg/mL; GRO alpha: 11.4-46500 pg/mL; IP-10: 4.9-20100 pg/mL; MCP-1: 18.03-75000 pg/mL; MCP-3: 3.5-14200 pg/mL; MIP-1 alpha: 2.3-9500 pg/mL; MIP-2: 0.6-2400 pg/mL; Rantes: 85.5-350000 pg/mL

Assay Sensitivity
[As determined for Lot 1:] IL-1 alpha: 3.5 pg/mL; C-CSF (CSF-3): 0.3 pg/mL; IL-10: 1.6 pg/mL; IL-17A: 0.2 pg/mL; IL-1 beta: 1.1 pg/mL; IL-6: 1.9 pg/mL; TNF alpha: 0.4 pg/mL; IL-4: 0.1 pg/mL; GM-CSF: 1.3 pg/mL; IFN gamma: 0.6 pg/mL; IL-2: 1.9 pg/mL; IL-5: 0.3 pg/mL; IL-13: 0.3 pg/mL; IL-12p70: 5.9 pg/mL; Eotaxin: 1.1 pg/mL; GRO alpha: 1.4 pg/mL; IP-10: 0.9 pg/mL; MCP-1: 10.9 pg/mL; MCP-3: 1.1 pg/mL; MIP-1 alpha: 1.0 pg/mL; MIP-2: 0.1 pg/mL; Rantes: 6.1 pg/mL

High Sensitivity 9-Plex Human ProcartaPlex™ Panel (Invitrogen™)

ProcartaPlex™ immunoassays are our ideal solution for assessing multiple protein biomarkers in a single sample. Our ProcartaPlex assays use Luminex ™ xMAP technology for the multi-analyte detection of secreted proteins including cytokines, chemokines, growth factors and other proteins.
ProcartaPlex™ assays are subject to the same stringent and robust development and manufacturing as are our extensively validated Platinum and Instant ELISA kits. Quantitate multiple targets in cell/tissue culture supernatants, serum, and plasma.

Explore our broad portfolio of pre-configurated panels and combinable single analytes.

NOTE: This kit cannot be combined with other ProcartaPlex High Sensitivity Simplex Kits.

This kit does not contain a Multiscreen Filter Plate.

Reactivity/Species
Human

Suitable Sample Types
cell culture supernatant, serum, plasma

Sample Volume
Serum, Plasma: 25 µL; CCS: 50 µL

Reported Application
Multiplex Immunoassay

Target List
IFN gamma; IL-1 beta; IL-2; IL-4; IL-6; IL-10; IL-12p70; IL-17A; TNF alpha

Bead Regions
See product documentation

Assay Range
[As determined for Lot 1:] IFN gamma: 0.31-1250 pg/mL; IL-1 beta: 0.32-1330 pg/mL; IL-2: 0.44-1820 pg/mL; IL-4: 0.86-3530 pg/mL; IL-6: 1.03-4230 pg/mL; IL-10: 0.18-750 pg/mL; IL-12p70: 0.75-3090 pg/mL; IL-17A: 0.25-1030 pg/mL; TNF alpha: 0.45-1830 pg/mL

Assay Sensitivity
[As determined for Lot 1:] IFN gamma: 0.075 pg/mL; IL-12p70: 0.044 pg/mL; IL-1 beta: 0.145 pg/mL; IL-2: 0.199 pg/mL; IL-4: 0.237 pg/mL; IL-6: 0.038 pg/mL; TNF alpha: 0.215 pg/mL; IL-10: 0.039 pg/mL; IL-17A: 0.016 pg/mL

Cytokine & Chemokine 26-Plex Mouse ProcartaPlex™ Panel 1 (Invitrogen™)

The Mouse Cytokine & Chemokine 26-plex ProcartaPlex Panel is a preconfigured multiplex immunoassay kit that measures 26 protein targets using Luminex xMAP technology. ProcartaPlex preconfigured panels are supplied with all the necessary reagents to perform the assay and are validated to ensure reproducibility and consistency. These panels are designed for those who need greater validation for longitudinal studies.

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate all protein targets simultaneously using a Luminex instrument. ProcartaPlex multiplex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

Reproducible, reliable results—validated as a panel to the highest industry standard, including protein target combinability and cross-reactivity testing
More results per sample—measure multiple protein targets simultaneously in a single 25–50 µL sample
Well-established Luminex technology—the most referenced multiplexing platform for protein detection and quantitation

ProcartaPlex assays utilize Luminex xMAP (multianalyte profiling) technology for the simultaneous detection and quantitation of up to 80 protein targets in a single 25–50 µL sample—from plasma, serum, cell culture supernatants, and other bodily fluids.

The Luminex MagPlex superparamagnetic microsphere beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create 100 spectrally unique signatures that can be identified by the Luminex xMAP detec¬tion systems (Luminex 200, FLEXMAP 3D, and MAGPIX systems). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a multiplexed assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a magnetic bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex multiplex panels are available in multiple formats across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information and available products.

Reactivity/Species
Mouse

Suitable Sample Types
cell culture supernatant, serum, plasma

Sample Volume
Serum, Plasma: 25 µL; CCS: 50 µL

Reported Application
Multiplex Immunoassay

Target List
IFN gamma; IL-12p70; IL-13; IL-1 beta; IL-2; IL-4; IL-5; IL-6; TNF alpha; GM-CSF; IL-18; IL-10; IL-17A; IL-22; IL-23; IL-27; IL-9; GRO alpha; IP-10; MCP-1; MCP-3; MIP-1 alpha; MIP-1 beta; MIP-2; RANTES; Eotaxin

Bead Regions
IFN gamma: 38; IL-12p70: 39; IL-13: 35; IL-1 beta: 19; IL-2: 20; IL-4: 26; IL-5: 27; IL-6: 28; TNF alpha: 45; GM-CSF: 42; IL-18: 66; IL-10: 13; IL-17A: 52 ; IL-22: 33; IL-23: 37; IL-27: 36; IL-9: 34; GRO alpha: 43; IP-10: 22; MCP-1: 51; MCP-3: 48; MIP-1 alpha: 47; MIP-1 beta: 72; MIP-2: 55; RANTES: 44; Eotaxin: 62

Assay Range
See product documentation

Assay Sensitivity
See product documentation

Cytokine & Chemokine 34-Plex Human ProcartaPlex™ Panel 1A (Invitrogen™)

The Human Cytokine & Chemokine 34-plex ProcartaPlex Panel is a preconfigured multiplex immunoassay kit that measures 34 protein targets using Luminex xMAP technology. ProcartaPlex preconfigured panels are supplied with all the necessary reagents to perform the assay and are validated to ensure reproducibility and consistency. These panels are designed for those who need greater validation for longitudinal studies.

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate all protein targets simultaneously using a Luminex instrument. ProcartaPlex multiplex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

Reproducible, reliable results—validated as a panel to the highest industry standard, including protein target combinability and cross-reactivity testing
More results per sample—measure multiple protein targets simultaneously in a single 25–50 µL sample
Well-established Luminex technology—the most referenced multiplexing platform for protein detection and quantitation

ProcartaPlex assays utilize Luminex xMAP (multianalyte profiling) technology for the simultaneous detection and quantitation of up to 80 protein targets in a single 25–50 µL sample—from plasma, serum, cell culture supernatants, and other bodily fluids.

The Luminex MagPlex superparamagnetic microsphere beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create 100 spectrally unique signatures that can be identified by the Luminex xMAP detec¬tion systems (Luminex 200, FLEXMAP 3D, and MAGPIX systems). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a multiplexed assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a magnetic bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex multiplex panels are available in multiple formats across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information and available products.

Reactivity/Species
Human

Suitable Sample Types
cell culture supernatant, serum, plasma

Sample Volume
Serum, Plasma: 25 µL; CCS: 50 µL

Reported Application
Multiplex Immunoassay

Target List
Eotaxin/CCL11; GM-CSF; GRO alpha/CXCL1; IFN alpha; IFN gamma; IL-1 beta; IL-1 alpha; IL-1RA; IL-2; IL-4; IL-5; IL-6; IL-7; IL-8/CXCL8; IL-9; IL-10; IL-12 p70; IL-13; IL-15; IL-17A; IL-18; IL-21; IL-22; IL-23; IL-27; IL-31; IP-10/CXCL10; MCP-1/CCL2; MIP-1 alpha/CCL3; MIP-1 beta/CCL4; RANTES/CCL5; SDF1 alpha/CXCL12; TNF alpha; TNF beta/LTA

Bead Regions
IFN gamma: 43; IL-12p70: 34 ; IL-13: 35; IL-1 beta: 18; IL-2: 19; IL-4: 20; IL-5: 21; IL-6: 25; TNF alpha: 45; GM-CSF: 44; IL-18: 66; IL-10: 28; IL-17A: 36; IL-21: 72; IL-22: 76; IL-23: 63; IL-27: 14; IL-9: 52; IFN alpha: 48; IL-31: 37; IL-15: 65; IL-1 alpha: 62; IL-1RA: 38; IL-7: 26; TNF beta: 54; Eotaxin: 33; GRO alpha: 61; IL-8: 27; IP-10: 22; MCP-1: 51; MIP-1 alpha: 12; MIP-1 beta: 47; RANTES: 42; SDF-1 alpha: 13

Assay Range
See product documentation

Assay Sensitivity
See product documentation

Th1/Th2 Cytokine 11-Plex Human ProcartaPlex™ Panel (Invitrogen™)

The Human Th1/Th2 Cytokine 11-plex ProcartaPlex Panel is a preconfigured multiplex immunoassay kit that measures 11 protein targets using Luminex xMAP technology. ProcartaPlex preconfigured panels are supplied with all the necessary reagents to perform the assay and are validated to ensure reproducibility and consistency. These panels are designed for those who need greater validation for longitudinal studies.

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate all protein targets simultaneously using a Luminex instrument. ProcartaPlex multiplex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

Reproducible, reliable results—validated as a panel to the highest industry standard, including protein target combinability and cross-reactivity testing
More results per sample—measure multiple protein targets simultaneously in a single 25–50 µL sample
Well-established Luminex technology—the most referenced multiplexing platform for protein detection and quantitation

ProcartaPlex assays utilize Luminex xMAP (multianalyte profiling) technology for the simultaneous detection and quantitation of up to 80 protein targets in a single 25–50 µL sample—from plasma, serum, cell culture supernatants, and other bodily fluids.

The Luminex MagPlex superparamagnetic microsphere beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create 100 spectrally unique signatures that can be identified by the Luminex xMAP detec¬tion systems (Luminex 200, FLEXMAP 3D, and MAGPIX systems). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a multiplexed assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a magnetic bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex multiplex panels are available in multiple formats across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information and available products.

Reactivity/Species
Human

Suitable Sample Types
cell culture supernatant, serum, plasma

Sample Volume
Serum, Plasma: 25 µL; CCS: 50 µL

Reported Application
Multiplex Immunoassay

Target List
GM-CSF; IFN gamma; IL-1 beta; IL-2; IL-4; IL-5; IL-6; IL-12 p70; IL-13; IL-18; TNF alpha

Bead Regions
IFN gamma: 43; IL-12p70: 34; IL-13: 35; IL-1 beta: 18; IL-2: 19; IL-4: 20; IL-5: 21; IL-6: 25; TNF alpha: 45; GM-CSF: 44; IL-18: 66

Assay Range
[As determined for Lot 1:] IFN gamma: 12.21-50000 pg/mL; IL-12p70: 6.84-28000 pg/mL; IL-13: 2.44-10000 pg/mL; IL-1 beta: 2.44-10000 pg/mL; IL-2: 4.88-20000 pg/mL; IL-4: 12.21-50000 pg/mL; IL-5: 7.32-30000 pg/mL; IL-6: 9.77-40000 pg/mL; TNF alpha: 8.54-35000 pg/mL; GM-SCF: 17.09-70000 pg/mL; IL-18: 9.77-40000 pg/mL

Assay Sensitivity
[As determined for Lot 1:] IFN gamma: 0.2 pg/mL; IL-12p70: 0.04 pg/mL; IL-13: 0.1 pg/ml; IL-1 beta: 0.2 pg/mL; IL-2: 0.8 pg/mL; IL-4: 1.5 pg/mL; IL-5: 0.3 pg/mL; IL-6: 0.4 pg/mL; TNF alpha: 0.4 pg/mL; GM-CSF: 1.2 pg/mL; IL-18: 0.4 pg/mL