Sort By

IL1B Recombinant Mouse Protein (Gibco™)

Recombinant IL1B is a bioactive protein intended for use in cell culture applications. IL-1 is involved in a number of biological process including the stimulation of T-helper cells, promotion proliferation and synthesis of immunoglobulins, and the activation of fibroblasts and NK cells.

IL1B Recombinant Human Protein (Gibco™)

Recombinant Human Interleukin 1, beta (IL1B) is a bioactive protein intended for use in cell culture applications. IL-1 is involved in a number of biological processes including the stimulation of T-helper cells, promotion proliferation and synthesis of immunoglobulins, and the activation of fibroblasts and NK cells.

High purity—no interference from other proteins or contaminants
High biological activity—more results with less protein
Proven compatibility—Gibco® proteins bioassayed with Gibco® media

High Purity Recombinant Bioactive Protein
Recombinant Human Interleukin 1, beta is one of our more than 250 available Gibco® recombinant proteins. To help ensure Gibco® growth factors are of the highest quality, each protein is analyzed for purity along with structural homogeneity to ensure a biologically active protein.

High Biological Activity
In-house activity testing of Gibco® Recombinant Proteins includes cell proliferation, cytotoxicity, chemotaxis, calcium flux, secondary cytokine up-regulation, induction of surface antigen expression, antiviral, and protease assays. Recombinant Human Interleukin 1, beta (IL1B) specific activity is determined by the dose dependent proliferation of murine D10S cells.

Proven Compatibility
Gibco® proteins are bioassayed with Gibco® media. Since 1962, Gibco® has been the consistent provider of high quality media, reagents, and sera for reliable cell culture.

See our selection of recombinant Gibco® growth factors.

For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.

IL1B Recombinant Rat Protein (Gibco™)

Recombinant IL1B is a bioactive protein intended for use in cell culture applications. IL-1 is involved in a number of biological process including the stimulation of T-helper cells, promotion proliferation and synthesis of immunoglobulins, and the activation of fibroblasts and NK cells.

IL1B Monoclonal Antibody (OTI3E1), TrueMAB™ (OriGene)

IL1B Monoclonal Antibody for Western blot, IHC (P), IF, ICC

IL1B Monoclonal Antibody (OTI1A7), TrueMAB™ (OriGene)

IL1B Monoclonal Antibody for Western blot

IL-1 beta Rat Uncoated ELISA Kit with Plates (Invitrogen™)

IL-1 beta Rat Uncoated ELISA Kit with Plates for ELISA

IL-1 beta Rat ELISA Kit (Invitrogen™)

IL-1 beta-Cell Lysates Rat ELISA Kit for ELISA

IL-2 Human Uncoated ELISA Kit with Plates (Invitrogen™)

IL-2 Human Uncoated ELISA Kit with Plates for ELISA

Bovine IL-1 beta Recombinant Protein (Invitrogen™)

Bovine IL-1 beta Recombinant Protein for Western Blot, IP, ELISA

IL-1 beta Mouse Uncoated ELISA Kit with Plates (Invitrogen™)

IL-1 beta Pro-form Mouse Uncoated ELISA Kit with Plates for ELISA

IL-1 beta Polyclonal Antibody (Invitrogen™)

IL-1 beta Polyclonal Antibody for Western Blot

IL-1 beta Human ELISA Kit (Invitrogen™)

IL-1 beta Human ELISA Kit for ELISA

IL-1 beta Human ProcartaPlex™ Simplex Kit, Platinum (Invitrogen™)

Platinum ProcartaPlex™ Immunoassays are our are highest validated bead based Multiplexing assay system for quantificaton of multiple protein biomarkers in a single sample.
Newly developed matrix type specific diluents for plasma and serum enable to overcome matrix effects and assure high performance specifications (e.g spike and dilution recovery results between 70-130%).
Our Platinum ProcartaPlex assays use Luminex™ xMAP technology for the multi-analyte detection of secreted proteins including cytokines, chemokines, growth factors and other proteins. Platinum ProcartaPlex™ assays are subject to the same stringent and robust development and manufacturing as are our extensively validated Platinum and Instant ELISA assays.

Platinum ProcartaPlex Simplex kits can only be combined with one another.

Reactivity/Species
Human

Suitable Sample Types
Serum, Plasma

Sample Volume
Serum, Plasma: 25 µL

Reported Application
Multiplex Immunoassay

IL-1 beta Monoclonal Antibody (1400.24.17), Biotin (Invitrogen™)

IL-1 beta Monoclonal Antibody for Western Blot, ELISA

Interleukin 1 Beta

Interleukin 1, beta (IL1B), a recombinant human protein, is supplied as a lyophilized powder. It is suitable for use in protein studies such as protein structure analysis and protein-protein interactions. In general, recombinant proteins can also be used as immunogens, protein standards, and in other cell biology research applications.

This recombinant protein was expressed from a DNA sequence encoding the mature form of human IL1B (NP_000567.1) (Ala 117-Ser 269).


Activity:
1.Measured by its binding ability in a functional ELISA.
a. Immobilized recombinant human IL1B at 2 µg/ml (100µl/well) can bind recombinant human IL1R2 with a linear range of 6.25-400 ng/ml.

b. Immobilized human IL1B at 10 µg/ml (100 µl/well) can bind human IL1R1, The EC50 of human IL1R1 is 0.48 µg/ml.

2. Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED50 for this effect is typically 0.5-5 pg/ml.

Formulation: Lyophilized in 140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.

This protein is manufactured by Sino Biological Inc.