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Growth factors & recombinant proteins for cell culture

Our Gibco growth factors and Invitrogen recombinant proteins are produced in a range of expression systems including mammalian, E. coli, insect, and baculovirus to meet different cell culture application needs. Offering proven compatibility with Gibco cell culture media, these growth factors and recombinant proteins are designed to deliver superior results with less protein and with minimized interference from proteins or other contaminants.

Genomics and proteomics search results

IL6 Recombinant Human Protein (Gibco™)

Recombinant Human Interleukin 6 (IL6) is a bioactive protein intended for use in cell culture applications. IL6 is a pleiotropic cytokine involved in several cellular processes including inflammation, hematopoeisis, and immune response.

• High purity—no interference from other proteins or contaminants
• High biological activity—more results with less protein
• Proven compatibility—Gibco® proteins bioassayed with Gibco® media

High Purity Recombinant Bioactive Protein
Recombinant Human Interleukin 6 is one of our more than 250 available Gibco® recombinant proteins. To help ensure Gibco® growth factors are of the highest quality, each protein is analyzed for purity along with structural homogeneity to ensure a biologically active protein.

High Biological Activity
In-house activity testing of Gibco® Recombinant Proteins includes cell proliferation, cytotoxicity, chemotaxis, calcium flux, secondary cytokine up-regulation, induction of surface antigen expression, antiviral, and protease assays. Recombinant Human Interleukin 6 (IL6) specific activity is determined by the dose dependent proliferation of mouse B9 cells.

Proven Compatibility
Gibco® proteins are bioassayed with Gibco® media. Since 1962, Gibco® has been the consistent provider of high quality media, reagents, and sera for reliable cell culture.

See our selection of recombinant Gibco® growth factors.

For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.

IL6 (Interleukin 6) CTS™ Recombinant Human Protein (Gibco™)

Cell Therapy Systems (CTS™) Recombinant Human Interleukin 6 (IL6) is a bioactive protein intended for use in cell culture applications, including pre-clinical and clinical cell therapy studies. IL6 is a pleotropic cytokine involved in several cellular processes including inflammation, hematopoeisis, and immune response. The lyophilized, E. coli-expressed IL6 CTS™ Recombinant Human is verified for activity through dose-dependent proliferation of mouse B9 cells.

• Qualified—helps reduce your burden in qualifying reagents for regulatory submission in cell therapy applications
• Highly Pure—minimizes interference from other proteins or contaminants
• Active—get more results with less protein

Simplified Transition into Clinical Research
GIBCO® Cell Therapy Systems (CTS™) products are of high quality and supplied with harmonized documentation such as Certificates of Analysis, Certificates of Origin, and access to authorization letters for our Drug Master Files, as appropriate. The GIBCO® CTS™ product line enables you to reduce your burden in qualifying reagents during your transition from research applications to clinical applications.

High Purity
To help verify that GIBCO® CTS™ Recombinant growth factors are of the highest quality, each protein is analyzed for purity, along with structural homogeneity, to help ensure a biologically active protein. The purity of each GIBCO® CTS™ recombinant growth factor is verified by SDS-PAGE to be over 95% pure and to have endotoxin levels below 0.1 ng/μg.

High Biological Activity
All GIBCO® CTS™ Recombinant growth factors are verified for activity. Each lot has its own ED50 determined using an activity assay based on a specific function of the growth factor. The activity of our IL6 CTS™ Recombinant Human growth factor is determined by the dose-dependent proliferation of mouse B9 cells to have an ED50 range of 0.008–0.02 ng/mL (specific activity: 1.25 × 10^8–5.0 × 10^7 units/mg). Optimal concentration for individual applications should be determined by a dose response assay.

Related Links
• Learn More About GIBCO® Cell Therapy Systems (CTS™) Products
• Cell Therapy Systems Technical Support Web Page
• Our Full Collection of GIBCO® CTS™ Growth Factors
• Our Full Collection of GIBCO® Growth Factors

IL6 Recombinant Mouse Protein (Gibco™)

Recombinant Mouse Interleukin 6 (IL6) is a bioactive protein intended for use in cell culture applications. IL6 is a pleiotropic cytokine involved in several cellular processes including inflammation, hematopoeisis, and immune response.

• High purity—no interference from other proteins or contaminants
• High biological activity—more results with less protein
• Proven compatibility—Gibco® proteins bioassayed with Gibco® media

High Purity Recombinant Bioactive Protein
Recombinant Mouse Interleukin 6 is one of our more than 250 available Gibco® recombinant proteins. To help ensure Gibco® growth factors are of the highest quality, each protein is analyzed for purity along with structural homogeneity to ensure a biologically active protein.

High Biological Activity
In-house activity testing of Gibco® Recombinant Proteins includes cell proliferation, cytotoxicity, chemotaxis, calcium flux, secondary cytokine up-regulation, induction of surface antigen expression, antiviral, and protease assays. Recombinant Mouse Interleukin 6 (IL6) specific activity is determined by the dose dependent proliferation of mouse B9 cells.

Proven Compatibility
Gibco® proteins are bioassayed with Gibco® media. Since 1962, Gibco® has been the consistent provider of high quality media, reagents, and sera for reliable cell culture.

See our selection of recombinant Gibco® growth factors.

For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.

IL6 Recombinant Human Protein

Manufactured by Sino Biological (China); Distributed by Life Technologies.

Interleukin 6 (IL-6) recombinant human protein is supplied as a lyophilized powder. This protein is suitable for use in analysis of protein structure and performing cell based assays. It can also be used as an immunogen, as a protein standard, or in other research applications.

This recombinant protein is expressed from a DNA sequence encoding the mature form of human IL6 (NP_000591.1) (Val 30-Met 212) retaining the initial Met at the N-terminus.

N-terminal Sequence Analysis: Met.

Activity:
1. Measured by its binding ability in a functional ELISA. Immobilized recombinant human IL6 (100 µL/well of a 8 µg/mL solution) has been shown to bind recombinant human IL6R with a linear range of 1.25-20.0 ng/mL.
2. Measured in a cell proliferation assay using TF1 human erythroleukemic cells. The EC₅₀ for this effect is typically 0.2-0.8 ng/mL.

Formulation: lyophilized in 140 mM NaCl, 2.7 mM KCl, 10 mM Na₂HPO₄, 1.8 mM KH₂PO₄, pH 7.4, 5% mannitol, 5% trehalose, 0.02% Tween®-80.

Reconstitution: Dissolve the protein in sterile double-distilled water to a concentration of 0.2 mg/mL or lower. It is recommended that the protein be aliquoted and used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: expires one year from date of receipt when stored as instructed.

Background
Interleukin-6 (IL-6) is a multifunctional α-helical cytokine that regulates cell growth and differentiation of various tissues, and is known particularly for its role in the immune response and acute phase reactions. IL-6 is also involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined an essential role in directing transition from innate to acquired immunity. IL-6 is secreted by a variety of cell types including T cells and macrophages as phosphorylated and variably glycosylated molecule with the molecular mass of 22 kDa-28 kDa. Three IL-6 signaling pathways (STAT1, STAT3, and Ras-dependent MAPK cascade) have been reported. Dysregulated production of IL-6 and IL6R are implicated in the pathogenesis of several inflammatory diseases and malignancies such as multiple myeloma, rheumatoid arthritis, or osteoporosis.

You may also be interested in the following IL6 products:
ELISA Kits
Primary Antibodies
Gene Expression Assays

TaqMan™ Array Human IL6 Pathway (Applied Biosystems™)

These 96-well plates are pre-configured with the most appropriate TaqMan® Gene Expression Assays for a specific biological process, pathway, or disease state. Each plate contains predefined assays and endogenous controls dried-down in the wells, ready for accurate assessment of an entire gene signature in one simple experiment. The TaqMan® Array Human IL-6 Pathway 96-well Plate contains 92 assays to genes associated with IL-6 pathways and 4 assays to candidate endogenous control genes.

IL6 Recombinant Swine Protein (Gibco™)

Recombinant IL6 is a bioactive protein intended for use in cell culture applications. Recombinant human IL6 is intended for use in cell culture applications. IL6 is a pleotropic cytokine involved in several cellular processes including inflammation, hematopoeisis, and immune response.