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D-Myo-Inositol 1,4,5-Triphosphate, P4(5)-(1-(2-Nitrophenyl)ethyl) Ester, tris(triethylammonium) Salt (NPE-Caged Ins 1,4,5-P3) (Invitrogen™)

NPE-caged inositol 1,4,5-triphosphate is biologically inactive before photolysis. Flash photolysis of the caging group with UV illumination at 355 nm results in a rapid and highly localized release of free INS 1,4,5-P3 at the site of illumination.

B-27™ Supplement, minus insulin (Gibco™)

A variation of the most cited neuronal cell culture supplement, B-27 Supplement Minus Insulin is a customized version of the original B-27 Supplement without insulin. B-27 Supplement Minus Insulin is a serum-free supplement designed to support growth of hippocampal and other CNS neurons in applications where the presence of insulin causes interference. B-27 Supplement Minus Insulin is provided as a 50X liquid and is intended for use with Neurobasal Medium or Neurobasal-A Medium.

B-27 Supplement is suitable for use in most neuronal cell applications. For other applications, we provide alternative formulations of B-27 Supplement and Neurobasal media.

For established protocols using B-27 Supplement, access our Gibco Neurobiology Protocol Handbook.

View publications using Gibco neurobiology products at our Gibco CellCite.

Insulin Monoclonal Antibody (ICBTACLS), Biotin, eBioscience™ (Invitrogen™)

Insulin Monoclonal Antibody for IHC (P)

Lab Vision™ Insulin Receptor (α -Subunit) Ab-2, Mouse Monoclonal Antibody (Thermo Scientific™)

Ensure accurate, reproducible results in experiments with Thermo Scientific™ Insulin Receptor (α-Subunit) Ab-2, Mouse Monoclonal Antibody.

Insulin Monoclonal Antibody (ICBTACLS), eBioscience™ (Invitrogen™)

Insulin Monoclonal Antibody for Western Blot, IHC (P)

Lab Vision™ Insulin Receptor (β -Subunit) Ab-5, Mouse Monoclonal Antibody (Thermo Scientific™)

Recommended for a variety of molecular biology techniques, Thermo Scientific™ Insulin Receptor (β-Subunit) Ab-5, Mouse Monoclonal Antibody provides accurate, reproducible results.

Lab Vision™ Insulin Receptor (α -Subunit) Ab-1, Mouse Monoclonal Antibody (Thermo Scientific™)

Ensure accurate, reproducible results in experiments with the Thermo Scientific™ Insulin Receptor (α-Subunit) Ab-1, Mouse Monoclonal Antibody.

CaptureSelect™ Biotin Anti-Insulin Conjugate (Thermo Scientific™)

CaptureSelect™ Biotin Anti-Insulin Conjugate consists of a 13 kDa camelid antibody fragment (affinity ligand) with high affinity and selectivity for both recombinant and natural-sourced human insulin. The affinity ligand shows similar binding reactivity towards porcine- and bovine-sourced insulin. It also binds to human proinsulin, the precursor of insulin that still contains the C-peptide. The affinity ligand is chemically conjugated to biotin via an appropriate spacer that retains the binding reactivity of the ligand when used in combination with streptavidin-based conjugates or streptavidin pre-coated surfaces.

Applications for CaptureSelect™ Biotin Anti-Insulin Conjugate include ELISA, western blot, Gyrolab™-based immunoassays, and use of label-free detection platforms such as those based on surface plasmon resonance (Biacore™ and IBIS-MX96 systems) and bio-layer interferometry (ForteBio™ Octet™ systems).

Insulin Monoclonal Antibody (ICBTACLS), Alexa Fluor 488, eBioscience™ (Invitrogen™)

Insulin Monoclonal Antibody for IF, ICC, IHC (P)

Lab Vision™ Insulin Receptor (β -Subunit) Ab-6, Mouse Monoclonal Antibody (Thermo Scientific™)

Recommended for a variety molecular biology techniques, Thermo Scientific™ Insulin Receptor (β-Subunit) Ab-6, Mouse Monoclonal Antibody provides accurate, reproducible results.

C-Peptide Human ELISA Kit (Invitrogen™)

C-Peptide Human ELISA Kit

Insulin Rat ELISA Kit (Invitrogen™)

Insulin Rat ELISA Kit for ELISA

CTS™ B-27™ Supplement, XenoFree, minus insulin (Gibco™)

CTS B-27 Supplement XenoFree Minus Insulin is based on the classic B-27 Supplement formulation and contains no animal-derived components at the primary component level. The absence of insulin makes this formulation ideal for the growth of hippocampal and other CNS neurons in applications where the presence of insulin causes interference or in the derivation of cardiomyocytes and mesodermal cells from stem cells. CTS B-27 Supplement XenoFree Minus Insulin reduces the burden in qualifying reagents when transitioning from the research bench to the clinic.

CTS quality
Gibco CTS products are manufactured at a site that uses methods and controls that conform with cGMP for medical devices, 21 CFR Part 820. Our FDA-registered manufacturing sites are ISO 13485– and ISO 9001–certified.

Formulated xeno-free
The preparation contains no animal-derived components at the primary component level.

Accompanied by documentation to support your regulatory filing
Specific intended-use statements, full documentation traceability, and convenient access to our Drug Master File (DMF) are available.

For other applications, we provide alternative formulations of CTS B-27 supplements and CTS Neurobasal media.

For established protocols using B-27 Supplement, access our Gibco Neurobiology Protocol Handbook.

View publications using Gibco neurobiology products at our Gibco CellCite.

TaqMan™ Array Human Insulin Receptor Pathway (Applied Biosystems™)

These 96-well plates are pre-configured with the most appropriate TaqMan® Gene Expression Assays for a specific biological process, pathway, or disease state. Each plate contains predefined assays and endogenous controls dried-down in the wells, ready for accurate assessment of an entire gene signature in one simple experiment. The TaqMan® Array Human Insulin Receptor Pathway 96-well Plate contains 92 assays to genes associated with Insulin Receptor pathways and 4 assays to candidate endogenous control genes.

Insulin Monoclonal Antibody (ICBTACLS), eFluor 660, eBioscience™ (Invitrogen™)

Insulin Monoclonal Antibody for IF, ICC, IHC (P)

TaqMan™ Array, Human Insulin Receptor Pathway, Fast 96-well (Applied Biosystems™)

These 96-well plates are pre-configured with the most appropriate TaqMan® Gene Expression Assays for a specific biological process, pathway, or disease state. Each plate contains predefined assays and endogenous controls dried-down in the wells, ready for accurate assessment of an entire gene signature in one simple experiment. The TaqMan® Array Human Insulin Receptor Pathway 96-well Plate contains 92 assays to genes associated with Insulin Receptor pathways and 4 assays to candidate endogenous control genes.

Insulin Polyclonal Antibody (Invitrogen™)

Insulin Polyclonal Antibody for IHC (P)

Procollagen I C-Peptide Monoclonal Antibody (002-04) (Invitrogen™)

Procollagen I C-Peptide Monoclonal Antibody for ELISA

Procollagen I C-Peptide Monoclonal Antibody (18) (Invitrogen™)

Procollagen I C-Peptide Monoclonal Antibody for Western Blot, ELISA

Insulin Polyclonal Antibody (Invitrogen™)

Insulin Polyclonal Antibody for IHC (P), ELISA

Procollagen I C-Peptide Monoclonal Antibody (8) (Invitrogen™)

Procollagen I C-Peptide Monoclonal Antibody for ELISA

C-Peptide Polyclonal Antibody (Invitrogen™)

C-Peptide Polyclonal Antibody for IHC (P), ELISA

Human Insulin Recombinant Protein (Invitrogen™)

Human Insulin Recombinant Protein for Ctrl

Insulin Monoclonal Antibody (4B6A7) (Proteintech)

Insulin Monoclonal Antibody for IHC (P)

Insulin Polyclonal Antibody (Proteintech)

Insulin Polyclonal Antibody for Western Blot, IF, ICC, IHC (P)

Insulin Receptor Beta Polyclonal Antibody (Bethyl Laboratories)

Insulin Receptor Beta Polyclonal Antibody for Western Blot

Insulin Receptor Beta Polyclonal Antibody (Bethyl Laboratories)

Insulin Receptor Beta Polyclonal Antibody for Western Blot, IP

Insulin-Transferrin-Selenium (ITS -G) (100X) (Gibco™)

Gibco® Insulin-Transferrin-Selenium (ITS-G) is used as a basal medium supplement in order to reduce the amount of fetal bovine serum (FBS) needed to culture cells. Originally used with RPMI 1640 and Minimum Essential Medium (MEM), ITS-G solution is now routinely used with other basal media to support a wide variety of cell types in the presence of 2–4% FBS.

Insulin promotes glucose and amino acid uptake, lipogenesis, intracellular transport, and the synthesis of proteins and nucleic acids. Transferrin is an iron carrier and it may also help to reduce toxic levels of oxygen radicals and peroxide. Selenium, as sodium selenite, is a co-factor for glutathione peroxidase and other proteins, and is used as an anti-oxidant in media.

The complete formulation is available.

cGMP Manufacturing and Quality System
Gibco® ITS-G is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

Insulin-Transferrin-Selenium-Ethanolamine (ITS -X) (100X) (Gibco™)

Gibco® Insulin,-Transferrin-Selenium-Ethanolamine (ITS-X) is used as a basal medium supplement in order to reduce the amount of fetal bovine serum (FBS) used to culture cells. ITS-X solution is routinely used, in combination with 2–4% FBS, to support cell types that require ethanolamine, such as bronchial epithelial cells.

Insulin promotes glucose and amino acid uptake, lipogenesis, intracellular transport, and the synthesis of proteins and nucleic acids. Transferrin is an iron carrier and may also help to reduce toxic levels of oxygen radicals and peroxide. Selenium, as sodium selenite is a co-factor for glutathione peroxidase and other proteins that is used as an anti-oxidant in media. Ethanolamine is a precursor of phospho-glycerides which are essential to the structure of the plasma membrane and cellular organelles.

The complete formulation is available.

cGMP Manufacturing and Quality System
Gibco® ITS-X is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

Insulin-Transferrin-Selenium-Sodium Pyruvate (ITS-A) (100X) (Gibco™)

ITS-A (Insulin-Transferrin-Selenium-Sodium Pyruvate) Supplement is used as a basal medium supplement in order to reduce the amount of Fetal Bovine Serum (FBS) used to culture cells. ITS-A is used for adherent cell cultures. Originally used with RPMI 1640 and Minimum Essential Medium (MEM), ITS-A is now routinely used with other basal media to support a wide variety of cell types in the presence of 2–4% FBS. We also offer ITS-G and ITS-X supplements.

Using ITS-A Supplement
Insulin promotes glucose and amino acid uptake, lipogenesis, intracellular transport, and the synthesis of proteins and nucleic acids. Transferrin is an iron carrier and it may also help to reduce toxic levels of oxygen radicals and peroxide. Selenite is a co-factor for glutathione peroxidase and other proteins, and is used as an anti-oxidant in media. Sodium Pyruvate is a precursor to amino acids, fatty acids, and cholesterol; it is an important intermediate in many biosynthetic pathways. The complete formulation is available.

cGMP manufacturing and quality system
ITS-A Supplement is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.