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D-Myo-Inositol 1,4,5-Triphosphate, P4(5)-(1-(2-Nitrophenyl)ethyl) Ester, tris(triethylammonium) Salt (NPE-Caged Ins 1,4,5-P3) (Invitrogen™)

NPE-caged inositol 1,4,5-triphosphate is biologically inactive before photolysis. Flash photolysis of the caging group with UV illumination at 355 nm results in a rapid and highly localized release of free INS 1,4,5-P3 at the site of illumination.

Insulin Monoclonal Antibody (ICBTACLS), Biotin, eBioscience™ (Invitrogen™)

Insulin Monoclonal Antibody for IHC (P)

CaptureSelect™ Biotin Anti-Insulin Conjugate (Thermo Scientific™)

CaptureSelect™ Biotin Anti-Insulin Conjugate consists of a 13 kDa camelid antibody fragment (affinity ligand) with high affinity and selectivity for both recombinant and natural-sourced human insulin. The affinity ligand shows similar binding reactivity towards porcine- and bovine-sourced insulin. It also binds to human proinsulin, the precursor of insulin that still contains the C-peptide. The affinity ligand is chemically conjugated to biotin via an appropriate spacer that retains the binding reactivity of the ligand when used in combination with streptavidin-based conjugates or streptavidin pre-coated surfaces.

Applications for CaptureSelect™ Biotin Anti-Insulin Conjugate include ELISA, western blot, Gyrolab™-based immunoassays, and use of label-free detection platforms such as those based on surface plasmon resonance (Biacore™ and IBIS-MX96 systems) and bio-layer interferometry (ForteBio™ Octet™ systems).

Lab Vision™ Insulin Receptor (α -Subunit) Ab-1, Mouse Monoclonal Antibody (Thermo Scientific™)

Ensure accurate, reproducible results in experiments with the Thermo Scientific™ Insulin Receptor (α-Subunit) Ab-1, Mouse Monoclonal Antibody.

Insulin Monoclonal Antibody (ICBTACLS), Alexa Fluor 488, eBioscience™ (Invitrogen™)

Insulin Monoclonal Antibody for IF, ICC, IHC (P)

Insulin Monoclonal Antibody (ICBTACLS), eBioscience™ (Invitrogen™)

Insulin Monoclonal Antibody for Western Blot, IHC (P)

Lab Vision™ Insulin Receptor (β -Subunit) Ab-6, Mouse Monoclonal Antibody (Thermo Scientific™)

Recommended for a variety molecular biology techniques, Thermo Scientific™ Insulin Receptor (β-Subunit) Ab-6, Mouse Monoclonal Antibody provides accurate, reproducible results.

Insulin Rat ELISA Kit (Invitrogen™)

Insulin Rat ELISA Kit for ELISA

TaqMan™ Array Human Insulin Receptor Pathway (Applied Biosystems™)

These 96-well plates are pre-configured with the most appropriate TaqMan® Gene Expression Assays for a specific biological process, pathway, or disease state. Each plate contains predefined assays and endogenous controls dried-down in the wells, ready for accurate assessment of an entire gene signature in one simple experiment. The TaqMan® Array Human Insulin Receptor Pathway 96-well Plate contains 92 assays to genes associated with Insulin Receptor pathways and 4 assays to candidate endogenous control genes.

Lab Vision™ Insulin Receptor (α -Subunit) Ab-2, Mouse Monoclonal Antibody (Thermo Scientific™)

Ensure accurate, reproducible results in experiments with Thermo Scientific™ Insulin Receptor (α-Subunit) Ab-2, Mouse Monoclonal Antibody.

TaqMan™ Array, Human Insulin Receptor Pathway, Fast 96-well (Applied Biosystems™)

These 96-well plates are pre-configured with the most appropriate TaqMan® Gene Expression Assays for a specific biological process, pathway, or disease state. Each plate contains predefined assays and endogenous controls dried-down in the wells, ready for accurate assessment of an entire gene signature in one simple experiment. The TaqMan® Array Human Insulin Receptor Pathway 96-well Plate contains 92 assays to genes associated with Insulin Receptor pathways and 4 assays to candidate endogenous control genes.

B-27™ Supplement, minus insulin (Gibco™)

A variation of the most cited neuronal cell culture supplement, B-27 Supplement Minus Insulin is a customized version of the original B-27 Supplement without insulin. B-27 Supplement Minus Insulin is a serum-free supplement designed to support growth of hippocampal and other CNS neurons in applications where the presence of insulin causes interference. B-27 Supplement Minus Insulin is provided as a 50X liquid and is intended for use with Neurobasal Medium or Neurobasal-A Medium.

B-27 Supplement is suitable for use in most neuronal cell applications. For other applications, we provide alternative formulations of B-27 Supplement and Neurobasal media.

For established protocols using B-27 Supplement, access our Gibco Neurobiology Protocol Handbook.

View publications using Gibco neurobiology products at our Gibco CellCite.

Lab Vision™ Insulin Receptor (β -Subunit) Ab-5, Mouse Monoclonal Antibody (Thermo Scientific™)

Recommended for a variety of molecular biology techniques, Thermo Scientific™ Insulin Receptor (β-Subunit) Ab-5, Mouse Monoclonal Antibody provides accurate, reproducible results.

Insulin Monoclonal Antibody (ICBTACLS), eFluor 660, eBioscience™ (Invitrogen™)

Insulin Monoclonal Antibody for IF, ICC, IHC (P)

CTS™ B-27™ Supplement, XenoFree, minus insulin (Gibco™)

CTS B-27 Supplement XenoFree Minus Insulin is based on the classic B-27 Supplement formulation and contains no animal-derived components at the primary component level. The absence of insulin makes this formulation ideal for the growth of hippocampal and other CNS neurons in applications where the presence of insulin causes interference or in the derivation of cardiomyocytes and mesodermal cells from stem cells. CTS B-27 Supplement XenoFree Minus Insulin reduces the burden in qualifying reagents when transitioning from the research bench to the clinic.

CTS quality
Gibco CTS products are manufactured at a site that uses methods and controls that conform with cGMP for medical devices, 21 CFR Part 820. Our FDA-registered manufacturing sites are ISO 13485– and ISO 9001–certified.

Formulated xeno-free
The preparation contains no animal-derived components at the primary component level.

Accompanied by documentation to support your regulatory filing
Specific intended-use statements, full documentation traceability, and convenient access to our Drug Master File (DMF) are available.

For other applications, we provide alternative formulations of CTS B-27 supplements and CTS Neurobasal media.

For established protocols using B-27 Supplement, access our Gibco Neurobiology Protocol Handbook.

View publications using Gibco neurobiology products at our Gibco CellCite.

C-Peptide Human ELISA Kit (Invitrogen™)

C-Peptide Human ELISA Kit

Procollagen I C-Peptide Monoclonal Antibody (18) (Invitrogen™)

Procollagen I C-Peptide Monoclonal Antibody for Western Blot, ELISA

Procollagen I C-Peptide Monoclonal Antibody (8) (Invitrogen™)

Procollagen I C-Peptide Monoclonal Antibody for ELISA

Procollagen I C-Peptide Monoclonal Antibody (002-04) (Invitrogen™)

Procollagen I C-Peptide Monoclonal Antibody for ELISA

Insulin Polyclonal Antibody (Invitrogen™)

Insulin Polyclonal Antibody for IHC (P), ELISA

Insulin Polyclonal Antibody (Invitrogen™)

Insulin Polyclonal Antibody for IHC (P)

C-Peptide Polyclonal Antibody (Invitrogen™)

C-Peptide Polyclonal Antibody for IHC (P), ELISA

Human Insulin Recombinant Protein (Invitrogen™)

Human Insulin Recombinant Protein for Ctrl

Insulin Receptor Beta Polyclonal Antibody (Bethyl Laboratories)

Insulin Receptor Beta Polyclonal Antibody for Western Blot, IP

Insulin Receptor Beta Polyclonal Antibody (Bethyl Laboratories)

Insulin Receptor Beta Polyclonal Antibody for Western Blot

Insulin Monoclonal Antibody (4B6A7) (Proteintech)

Insulin Monoclonal Antibody for IHC (P)

Insulin Polyclonal Antibody (Proteintech)

Insulin Polyclonal Antibody for Western Blot, IF, ICC, IHC (P)

Insulin-Transferrin-Selenium-Ethanolamine (ITS -X) (100X) (Gibco™)

Gibco® Insulin,-Transferrin-Selenium-Ethanolamine (ITS-X) is used as a basal medium supplement in order to reduce the amount of fetal bovine serum (FBS) used to culture cells. ITS-X solution is routinely used, in combination with 2–4% FBS, to support cell types that require ethanolamine, such as bronchial epithelial cells.

Insulin promotes glucose and amino acid uptake, lipogenesis, intracellular transport, and the synthesis of proteins and nucleic acids. Transferrin is an iron carrier and may also help to reduce toxic levels of oxygen radicals and peroxide. Selenium, as sodium selenite is a co-factor for glutathione peroxidase and other proteins that is used as an anti-oxidant in media. Ethanolamine is a precursor of phospho-glycerides which are essential to the structure of the plasma membrane and cellular organelles.

The complete formulation is available.

cGMP Manufacturing and Quality System
Gibco® ITS-X is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

Insulin-Transferrin-Selenium-Sodium Pyruvate (ITS-A) (100X) (Gibco™)

ITS-A (Insulin-Transferrin-Selenium-Sodium Pyruvate) Supplement is used as a basal medium supplement in order to reduce the amount of Fetal Bovine Serum (FBS) used to culture cells. ITS-A is used for adherent cell cultures. Originally used with RPMI 1640 and Minimum Essential Medium (MEM), ITS-A is now routinely used with other basal media to support a wide variety of cell types in the presence of 2–4% FBS. We also offer ITS-G and ITS-X supplements.

Using ITS-A Supplement
Insulin promotes glucose and amino acid uptake, lipogenesis, intracellular transport, and the synthesis of proteins and nucleic acids. Transferrin is an iron carrier and it may also help to reduce toxic levels of oxygen radicals and peroxide. Selenite is a co-factor for glutathione peroxidase and other proteins, and is used as an anti-oxidant in media. Sodium Pyruvate is a precursor to amino acids, fatty acids, and cholesterol; it is an important intermediate in many biosynthetic pathways. The complete formulation is available.

cGMP manufacturing and quality system
ITS-A Supplement is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

Insulin-Transferrin-Selenium (ITS -G) (100X) (Gibco™)

Gibco® Insulin-Transferrin-Selenium (ITS-G) is used as a basal medium supplement in order to reduce the amount of fetal bovine serum (FBS) needed to culture cells. Originally used with RPMI 1640 and Minimum Essential Medium (MEM), ITS-G solution is now routinely used with other basal media to support a wide variety of cell types in the presence of 2–4% FBS.

Insulin promotes glucose and amino acid uptake, lipogenesis, intracellular transport, and the synthesis of proteins and nucleic acids. Transferrin is an iron carrier and it may also help to reduce toxic levels of oxygen radicals and peroxide. Selenium, as sodium selenite, is a co-factor for glutathione peroxidase and other proteins, and is used as an anti-oxidant in media.

The complete formulation is available.

cGMP Manufacturing and Quality System
Gibco® ITS-G is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

Insulin, human recombinant, zinc solution (Gibco™)

4 mg/ml

Potency: (dry basis) >= 27 USP units/mg.
Suggested range for supplementation: 2 to 20 µg/ml.

Iso-Insulin Human Instant ELISA™ Kit (Invitrogen™)

Iso-Insulin Human Instant ELISA™ Kit for ELISA

C-peptide Human ProcartaPlex™ Simplex Kit (Invitrogen™)

The Human C-peptide Simplex ProcartaPlex kit measures C-peptide protein and is designed to be combinable with other Simplex kits so that you can create your own multiplex panel that utilizes Luminex xMAP technology for protein detection/quantitation. When combining multiple Simplex kits (i.e., when you are not using a pre-configured Multiplex Panel), only one buffer kit (sold separately) is needed for each assay plate regardless of plex size.

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate up to 80 protein targets simultaneously when using the FLEXMAP 3D or Luminex 200 instrument and up to 50 protein targets when using the MAGPIX instrument. ProcartaPlex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

Flexible panels—design your own panels with Simplex kits to measure your own array of targets
More results per sample—measure up to 80 protein targets in a single 25–50 µL sample
Well-established Luminex technology—the most referenced multiplexing platform for protein detection and quantitation

The Luminex MagPlex superparamagnetic microsphere beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create 100 spectrally unique signatures that can be identified by the Luminex xMAP detec¬tion systems (Luminex 200, FLEXMAP 3D, and MAGPIX systems). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a ProcartaPlex multiplex assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a magnetic bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile up to 80 times more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex Simplex kits provide the ability to create your own unique panel . More than 90% of ProcartaPlex Simplex targets can be combined, providing you with superior flexibility when creating your own multiplex panel.

ProcartaPlex Simplex kits are available across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information, including a comprehensive list of individual protein targets.

Reactivity/Species
Human

Suitable Sample Types
cell culture supernatant, serum, plasma

Sample Volume
Serum, Plasma: 25 µL; CCS: 50 µL

Reported Application
Multiplex Immunoassay

Insulin Mouse ELISA Kit (Invitrogen™)

Insulin Mouse ELISA Kit for ELISA

Insulin Recombinant Rabbit Monoclonal Antibody (19H4L12) (Invitrogen™)

Insulin Recombinant Monoclonal Antibody for IF, ICC, IHC

Insulin Recombinant Polyclonal Antibody (19HCLC) (Invitrogen™)

Insulin Recombinant Polyclonal Antibody for IF, IHC

Insulin Human ProcartaPlex™ Simplex Kit (Invitrogen™)

The Human Insulin Simplex ProcartaPlex kit measures insulin protein and is designed to be combinable with other Simplex kits so that you can create your own multiplex panel that utilizes Luminex xMAP technology for protein detection/quantitation. When combining multiple Simplex kits (i.e., when you are not using a pre-configured Multiplex Panel), only one buffer kit (sold separately) is needed for each assay plate regardless of plex size.

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate up to 80 protein targets simultaneously when using the FLEXMAP 3D or Luminex 200 instrument and up to 50 protein targets when using the MAGPIX instrument. ProcartaPlex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

Flexible panels—design your own panels with Simplex kits to measure your own array of targets
More results per sample—measure up to 80 protein targets in a single 25–50 µL sample
Well-established Luminex technology—the most referenced multiplexing platform for protein detection and quantitation

The Luminex MagPlex superparamagnetic microsphere beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create 100 spectrally unique signatures that can be identified by the Luminex xMAP detec¬tion systems (Luminex 200, FLEXMAP 3D, and MAGPIX systems). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a ProcartaPlex multiplex assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a magnetic bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile up to 80 times more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex Simplex kits provide the ability to create your own unique panel . More than 90% of ProcartaPlex Simplex targets can be combined, providing you with superior flexibility when creating your own multiplex panel.

ProcartaPlex Simplex kits are available across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information, including a comprehensive list of individual protein targets.

Reactivity/Species
Human

Suitable Sample Types
cell culture supernatant, serum, plasma

Sample Volume
Serum, Plasma: 25 µL; CCS: 50 µL

Reported Application
Multiplex Immunoassay

Insulin Human ELISA Kit (Invitrogen™)

Insulin Human ELISA Kit for ELISA

Insulin Monoclonal Antibody (INS04 (E2-E3)), Biotin (Invitrogen™)

Insulin Monoclonal Antibody for IHC (P)

Insulin Monoclonal Antibody (INS05 (2D11-H5)) (Invitrogen™)

Insulin Monoclonal Antibody for IHC (P)

Insulin Monoclonal Antibody (INS04 (E2-E3)) (Invitrogen™)

Insulin Monoclonal Antibody for IHC (P)

Insulin/Proinsulin Monoclonal Antibody (INS04 + INS05) (Invitrogen™)

Insulin/Proinsulin Cocktail Antibody for IHC (P)

C-Peptide Monoclonal Antibody (C-PEP-01) (Invitrogen™)

C-Peptide Monoclonal Antibody for ICC, IHC (P), ELISA, RIA

C-Peptide Monoclonal Antibody (C-PEP-01), Biotin (Invitrogen™)

C-Peptide Monoclonal Antibody for IHC (P)

Insulin/Proinsulin Monoclonal Antibody (D3E7 (5B6/6)) (Invitrogen™)

Insulin/Proinsulin Monoclonal Antibody for IHC (F), IHC (P), ELISA

Insulin/Proinsulin Monoclonal Antibody (D6C4 (5E4/3)) (Invitrogen™)

Insulin/Proinsulin Monoclonal Antibody for IHC (F), ELISA

Insulin/Proinsulin Monoclonal Antibody (D3E7), Biotin (Invitrogen™)

Insulin/Proinsulin Monoclonal Antibody for IHC (F), ELISA

Insulin Polyclonal Antibody (Invitrogen™)

Insulin Polyclonal Antibody for IF, ICC, IHC (F), IHC (P)

Insulin/Proinsulin Monoclonal Antibody (D3E7) (Invitrogen™)

Insulin/Proinsulin Monoclonal Antibody for ELISA

C-Peptide Monoclonal Antibody (4H8) (Invitrogen™)

C-Peptide Monoclonal Antibody for ELISA, RIA

Proinsulin Monoclonal Antibody (1G4) (Invitrogen™)

Proinsulin Monoclonal Antibody for IHC (F), ELISA

Insulin Monoclonal Antibody (7F8) (Invitrogen™)

Insulin Monoclonal Antibody for ELISA

Proinsulin Monoclonal Antibody (7D6) (Invitrogen™)

Proinsulin Monoclonal Antibody for ELISA

Insulin Polyclonal Antibody (Invitrogen™)

Insulin Polyclonal Antibody for IHC (P)

Insulin Monoclonal Antibody (7F8 (E6E5)) (Invitrogen™)

Insulin Monoclonal Antibody for ELISA

C-Peptide Monoclonal Antibody (7E10) (Invitrogen™)

C-Peptide Monoclonal Antibody for ELISA, RIA

Proinsulin Monoclonal Antibody (3A1) (Invitrogen™)

Proinsulin Monoclonal Antibody for IF, ICC, IHC (F), IHC (P), ELISA, RIA

C-Peptide Monoclonal Antibody (5B8) (Invitrogen™)

C-Peptide Monoclonal Antibody for ELISA, RIA

Proinsulin Monoclonal Antibody (1D3) (Invitrogen™)

Proinsulin Monoclonal Antibody for ELISA, RIA

Proinsulin Monoclonal Antibody (1D6) (Invitrogen™)

Proinsulin Monoclonal Antibody for IHC, ELISA, RIA