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New developments in particle-based tests and immunoassays. Citations & References

  • Authors: Bangs LB
  • Journal: J Int Fed Clin Chem
  • PubMed ID: 10148953
Catalog # M8997

Immunoassay reagents for thyroid testing. 1. Synthesis of thyroxine conjugates. Citations & References

  • Authors: Adamczyk M, Fino L, Fishpaugh JR, Johnson DD, Mattingly PG
  • Journal: Bioconjug Chem
  • PubMed ID: 7849077
Catalog # A1353

Development and testing of radio and enzyme immunoassays for acidic fibroblast growth factor (aFGF). Citations & References

  • Authors: Caruelle D, Grassi J, Courty J, Groux-Muscatelli B, Pradelles P, Barritault D, Caruelle JP
  • Journal: Anal Biochem
  • PubMed ID: 3056106
Catalog #
  • S1534
  • S1553(Discontinued)

Brochure: CEDIA & DRI Drugs of Abuse Testing Immunoassays Brochure [EN] Product Literature

Can urine be tested for cytokine levels using multiplex bead-based immunoassays like ProcartaPlex assays? Product FAQ

Answer

Urine samples can be reliably analyzed using our multiplexed bead immunoassays. A 2-fold dilution of the urine sample in kit Assay Diluent is recommended prior to performing the assay (final sample dilution in the assay will be 4-fold) to improve analyte recovery.

Answer Id:: E5225

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A highly sensitive immuno-PCR assay for detecting Group A Streptococcus. Citations & References

  • Authors: Liang H, Cordova SE, Kieft TL, Rogelj S
  • Journal: J Immunol Methods
  • PubMed ID: 12969551
Catalog # S1534

Global Drug Abuse Trends: A summary of Journal publications on drugs of abuse testing immunoassays Product Literature

What species of non-human primates can be used with your NHP ProcartaPlex assays? Product FAQ

Answer

We have listed all the species we have tested in our Cross-reactivity Chart for NHP ProcartaPlex Simplex Kits. This chart can be found here (https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-assays-analysis/luminex-multiplex-assays/procartaplex-immunoassays/procartaplex-assays.html) by clicking on the link for Non-human Primate ProcartaPlex Panels.

Answer Id:: E16829

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Fluorometric polymerase chain reaction (PCR) enzyme-linked immunosorbent assay for quantification of immuno-PCR products in microplates. Citations & References

  • Authors: Niemeyer CM, Adler M, Blohm D
  • Journal: Anal Biochem
  • PubMed ID: 9056198
Catalog #

Testing UK blood donors for exposure to human parvovirus 4 using a time-resolved fluorescence immunoassay to screen sera and Western blot to confirm reactive samples. Citations & References

  • Authors: Maple PA, Beard S, Parry RP, Brown KE
  • Journal: Transfusion
  • PubMed ID: 23721256

Anti-peptide monoclonal antibodies generated for immuno-multiple reaction monitoring-mass spectrometry assays have a high probability of supporting Western blot and ELISA. Citations & References

  • Authors: Schoenherr RM, Saul RG, Whiteaker JR, Yan P, Whiteley GR, Paulovich AG
  • Journal: Mol Cell Proteomics
  • PubMed ID: 25512614

What is the difference between your Thermo Scientific PolySorp, MaxiSorp, and MiniSorp surfaces? Product FAQ

Answer

MaxiSorp, PolySorp and MiniSorp surfaces were developed for immunology assays:
- The MaxiSorp surface is a modified, highly charged polystyrene surface with high affinity for molecules with polar or hydrophilic groups. The surface has a high binding capacity for proteins, including globular antibodies in proper orientation. Thus, it offers very high sensitivity in double antibody "sandwich" tests.
- The PolySorp surface is more hydrophobic than the MaxiSorp surface. It has high affinity to molecules of a more hydrophobic character. This surface is particularly suited to non-protein antigens including virus antigens.
- The MiniSorp surface is a polyethylene surface with very low affinity to molecules of any type. This type of surface is ideal for the liquid phase immuno techniques.
- The MiniSorp surface is only available in a tube format, while the MaxiSorp and PolySorp surfaces are offered in both 96-well plate and module formats.

Answer Id:: E17760

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QMS Everolimus Product Specifications [EN] Product Literature

Which polystyrene microspheres can be used for an agglutination test? Product FAQ

Answer

We do not have any formal recommendations to provide. The choice depends upon the mode of detection (by eye or microscopy; colorimetric or fluorescence), the color, and the material to be bound upon the surface of the beads (the appropriate surface modification on the bead for either passive or covalent attachment).
Here are some references on this topic:
- Wanger, et al., Chapter 11 - Antigen and Antibody Testing. Microbiology and Molecular Diagnosis in Pathology, A Comprehensive Review for Board Preparation, Certification and Clinical Practice, 2017, 221-232
- Cantarero LA, Butler JE, and Osborne JW, The Adsorptive Characteristics of Proteins for Polystyrene and their Significance in Solid-Phase Immunoassays, Anal Biochem, 1980, 105:375-382
- Kawaguchi H, Sakamoto K, Ohtsuka Y, et al., Fundamental Study on Latex Reagents for Agglutination Tests, Biomat, 1989, 10:225-229

Answer Id:: E18309

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Bioluminescence enhanced enzyme immunoassay. New ultrasensitive detection systems for enzyme immunoassays, II. Citations & References

  • Authors: Geiger R, Miska W
  • Journal: J Clin Chem Clin Biochem
  • PubMed ID: 3549963
Catalog # E186
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