Product FAQ

Which agarose co-immunoprecipitation and pulldown kits do you offer?

Answer

Read more about our kits and select the one best for your experiments on the following page (http://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-assays-analysis/immunoprecipitation/immunoprecipitation-agarose-other-resins/agarose-co-ip-pull-down-kits.html).

Answer Id: E12996

Was this answer helpful?

Yes
No
Thank you for your response

Product FAQ

What types of Thermo Scientific Protein:Protein Interaction (PPI) kits are available?

Answer

There are Pull-Down Kits and Far-Western Kits. The Pull-Down Kits are very similar to an immunoprecipitation (IP), but instead of an antibody, a tagged bait protein [biotin (Cat. No. 21115) , GST (Cat. No. 21516) or 6xHis (Cat. No. 21277)] is used to bind the prey or target protein. This protein complex is then purified using an affinity column specific for the tagged bait protein. The Far Western Kits are similar to Western Blots, but again, instead of an antibody, a tagged non-immunoglobulin protein [biotin (Cat. No. 23500) or GST (Cat. No. 23505)] is used to bind to the prey or target protein for detection on the membrane.

Answer Id: E8659

Was this answer helpful?

Yes
No
Thank you for your response

Product Literature

BioProbes 78 Journal of Cell Biology Applications

Product Literature

Brochure: Your Samples, Powered by KingFisher Instruments – Automated Sample Preparation for DNA, RNA, Protein, or Cells

Product Literature

Thermo Scientific Magnetic Bead Technology Brochure

Product Literature

Catalog: Pierce Magnetic Beads

Manual / Product Insert

User Guide: Pierce c-Myc Tag IP/Co-IP Kit

Version: Jan. 2015
Catalog #

Manual / Product Insert

Product Sheet: Pierce IP Lysis Buffer

Version: A.0

Product Literature

Thermo Scientific Avidin-Biotin Technical Handbook

Product Literature

Handbook: Protein preparation handbook

Product Literature

Brochure: Tools and reagents for recombinant protein purification

Manual / Product Insert

mirVana™ miRNA Probe Construction Kit Instruction Manual

Version: 1550M VERSION 0509
Catalog #
  • AM1550
  • AM1550M(Discontinued)

Product Literature

Application Note: Automated pull-down of extracellular vesicles (EVs) on the KingFisher system using Dynabeads magnetic beads—standardizing EV capture and analysis

Product Literature

Thermo Scientific Protein Biology Products for Neurobiology Research Handbook

Manual / Product Insert

User Guide: Pierce qIP Protein Interaction Tluc Assay Reagents

Version: Jan. 2015
Catalog #
  • 82015(Discontinued)
  • 82016(Discontinued)