Product FAQ

Which agarose co-immunoprecipitation and pulldown kits do you offer?

Answer

Read more about our kits and select the one best for your experiments on the following page (http://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-assays-analysis/immunoprecipitation/immunoprecipitation-agarose-other-resins/agarose-co-ip-pull-down-kits.html).

Answer Id: E12996

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Product FAQ

What types of Thermo Scientific Protein:Protein Interaction (PPI) kits are available?

Answer

There are Pull-Down Kits and Far-Western Kits. The Pull-Down Kits are very similar to an immunoprecipitation (IP), but instead of an antibody, a tagged bait protein [biotin (Cat. No. 21115) , GST (Cat. No. 21516) or 6xHis (Cat. No. 21277)] is used to bind the prey or target protein. This protein complex is then purified using an affinity column specific for the tagged bait protein. The Far Western Kits are similar to Western Blots, but again, instead of an antibody, a tagged non-immunoglobulin protein [biotin (Cat. No. 23500) or GST (Cat. No. 23505)] is used to bind to the prey or target protein for detection on the membrane.

Answer Id: E8659

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Product Literature

Thermo Scientific Magnetic Bead Technology Brochure

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Catalog: Pierce Magnetic Beads

Manual / Product Insert

mirVana™ miRNA Probe Construction Kit Instruction Manual

Version: 1550M VERSION 0509
Catalog #
  • AM1550
  • AM1550M(Discontinued)

Manual / Product Insert

User Guide: Pierce qIP Protein Interaction Tluc Assay Reagents

Version: Jan. 2015
Catalog #
  • 82015(Discontinued)
  • 82016(Discontinued)

Citations & References

Method to identify efficiently cleaved, membrane-bound, functional HIV-1 (Human Immunodeficiency Virus-1) envelopes.

  • Authors: Samal S, Bansal M, Das S
  • Journal: MethodsX 2019; (6): 837-849
  • PubMed ID: 31049300

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Brochure: Tools and reagents for recombinant protein purification

Manual / Product Insert

Instruction Manual: mirVana™ Probe & Marker Kit

Version: 1554M Revision C 06.2012
Catalog #

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