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Brochure: Immunosuppressant Drug Monitoring [EN] Product Literature

In vitro evaluation of the effects of candidate immunosuppressive drugs: flow cytometry and quantitative real-time PCR as two independent and correlated read-outs. Citations & References

  • Authors: Flores MG; Zhang S; Ha A; Holm B; Reitz BA; Morris RE; Borie DC
  • Journal: Journal of Immunological Methods
Catalog #

Symbols Glossary for Microgenics package inserts [EN] Manual / Product Insert

Catalog #

Method: Therapeutic Drug Monitoring of 8 new anticancer agents by High-Performance Liquid Chromatography - Tandem Mass Spectrometry Product Literature

Method: Therapeutic Drug Monitoring of 9 new anticancer agents by High-Performance Liquid Chromatography-Tandem Mass Spectrometry Product Literature

T cell activation induces a noncoding RNA transcript sensitive to inhibition by immunosuppressant drugs and encoded by the proto-oncogene, BIC. Citations & References

  • Authors: Haasch D; Chen YW; Reilly RM; Chiou XG; Koterski S; Smith ML; Kroeger P; McWeeny K; Halbert DN; Mollison KW; Djuric SW; Trevillyan JM
  • Journal: Cellular Immunology
Catalog #
  • 4318739(Discontinued)
  • 4327059(Discontinued)
  • 4327058(Discontinued)

Drug Monitoring, Quality Controls and Instrumentation Catalog 2018 [EN] Product Literature

Pretransplant pharmacodynamic analysis of immunosuppressive agents using CFSE-based T-cell proliferation assay. Citations & References

  • Authors: Kurata Y, Kato M, Kuzuya T, Miwa Y, Iwasaki K, Haneda M, Amioka K, Watarai Y, Uchida K, Nakao A, Kobayashi T,
  • Journal: Clin Pharmacol Ther
  • PubMed ID: 19404248
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Effects of aminosalicylates and immunosuppressive agents on nitric oxide-dependent N-nitrosation reactions. Citations & References

  • Authors: Grisham MB, Miles AM
  • Journal: Biochem Pharmacol
  • PubMed ID: 8204107
Catalog # D7918(Discontinued)

Inhibition of protein tyrosine phosphorylation in T cells by a novel immunosuppressive agent, leflunomide. Citations & References

  • Authors: Xu X, Williams JW, Bremer EG, Finnegan A, Chong AS
  • Journal: J Biol Chem
  • PubMed ID: 7759480
Catalog #

Immunosuppression in cardiac graft rejection: A human in vitro model to study the potential use of new immunomodulatory drugs Citations & References

  • Authors: Crescioli, C; Squecco, R; Cosmi, L; Sottili, M; Gelmini, S; Borgogni, E; Sarchielli, E; Scolletta, S; Francini, F; Annunziato, F; Vannelli, GB; Serio, M
  • Journal: EXPERIMENTAL CELL RESEARCH

What factors can contribute to rapid cell death/culture failure? Product FAQ

Answer

There are a number of events that can contribute to this:

1. Incorrect CO2 levels-monitor the level of CO2 manually with a Fyrite kit, available from Bacharach (http://www.bacharach-inc.com/fyrite_analyzers.htm). Check if the manual readings concur with the readings displayed on the incubator. If the incubator has a trace readout, check the printout for fluctuations in CO2 level. Check the settings to insure that CO2 levels are set at appropriate levels for your cell line (usually between 5 and 10%). Check line connections frequently for leaks. Avoid frequent opening and closing of incubator doors.
2. Temperature fluctuations in the incubator-Monitor the temperature of incubator with a good thermometer inside the incubator.
3. Fungizone or other preventive antibiotics/antimycotics are present at toxic concentrations-use at recommended levels.
4. Humidity is incorrect-check the water level in the water pan. Humidity is vital to appropriate gas exchange for many types of cells and media (i.e., appropriate CO2 levels are largely irrelevant for most cultures if the humidity level is not high enough).
5. Incorrect osmotic pressure in medium-check osmolality of complete medium. Most mammalian cells can tolerate an osmolality of 260 to 350 mOsm/kg. Additions of reagents such as HEPES and drugs may affect osmolality.
6. Contamination by microorganisms-bacterial and fungal contaminations are usually easily visible; symptoms of mycoplasma contamination are more subtle, and careful monitoring of culture morphology and regular testing are necessary to detect this type of contamination.
7. Inappropriate medium is being used-double-check that the medium used is appropriate for your cell type and culture application. For example, ensure that the medium being used for serum-free culture is actually designed for serum-free culture; make sure that appropriate selective drugs are used at appropriate levels; check the expiration dates for the reagents being used; and store medium at appropriate temperatures in the dark.

Answer Id:: E11905

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Identification of novel targets of immunosuppressive agents by cDNA-based microarray analysis. Citations & References

  • Authors: Cristillo Anthony D; Bierer Barbara E;
  • Journal: J Biol Chem
  • PubMed ID: 11694517
Catalog # 15596026
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