Product FAQ

What are induced pluripotent stem cells?

Answer

Induced pluripotent stem cells (iPS or iPSCs) are pluripotent stem cells directly generated by introducing combination of genes coding for “reprogramming factors” into adult cells. These reprogramming factors include Oct4, Sox2, c-Myc, KLF4, NANOG, and LIN28. Yu, et al, generated iPS from a human mesenchymal cell line using lentiviral vectors carrying Oct4, Sox2, NANOG, and LIN28 genes (Science 318:1917 (2007)). Using a similar approach, Takahashi et al, generated iPS from human primary fibroblast cells by introducing genes coding for Oct3, Sox2, KLF4, and c-Myc into these cells (Cell 131:861 (2007)). iPS generated by reprogramming are similar to human ES cells in morphology, the capacity for unlimited proliferation, surface-antigen expression, gene expression, the ability to differentiate into cell types representing the three germ layers in vitro, and the ability to form teratomas after injection into SCID mice.

Answer Id: E12180

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Citations & References

A simple and efficient cryopreservation method for feeder-free dissociated human induced pluripotent stem cells and human embryonic stem cells.

  • Authors: Mollamohammadi S, Taei A, Pakzad M, Totonchi M, Seifinejad A, Masoudi N, Baharvand H
  • Journal: Hum Reprod 2009 10:2468-2476
  • PubMed ID: 19602515
Catalog #

Citations & References

Feeder-free derivation of induced pluripotent stem cells from adult human adipose stem cells

  • Authors: Sun, N; Panetta, NJ; Gupta, DM; Wilson, KD; Lee, A; Jia, F; Hu, S; Cherry, AM; Robbins, RC; Longaker, MT; Wu, JC
  • Journal: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2009 37:15720-15725

Citations & References

Derivation of primordial germ cells from human embryonic and induced pluripotent stem cells is significantly improved by coculture with human fetal gonadal cells.

  • Authors: Park TS, Galic Z, Conway AE, Lindgren A, van Handel BJ, Magnusson M, Richter L, Teitell MA, Mikkola HK, Lowry WE, Plath K, Clark AT
  • Journal: Stem Cells 2009 4:783-795
  • PubMed ID: 19350678

Citations & References

A simple and efficient cryopreservation method for feeder-free dissociated human induced pluripotent stem cells and human embryonic stem cells

  • Authors: Mollamohammadi, S; Taei, A; Pakzad, M; Totonchi, M; Seifinejad, A; Masoudi, N; Baharvand, H
  • Journal: HUMAN REPRODUCTION 2009 10:2468-2476

Citations & References

Differential methylation of tissue- and cancer-specific CpG island shores distinguishes human induced pluripotent stem cells, embryonic stem cells and fibroblasts.

  • Authors: Doi A, Park IH, Wen B, Murakami P, Aryee MJ, Irizarry R, Herb B, Ladd-Acosta C, Rho J, Loewer S, Miller J, Schlaeger T, Daley GQ, Feinberg AP
  • Journal: Nat Genet 2009; (41):12 1350-1353
  • PubMed ID: 19881528

Citations & References

Single transcription factor reprogramming of hair follicle dermal papilla cells to induced pluripotent stem cells.

  • Authors: Tsai SY, Bouwman BA, Ang YS, Kim SJ, Lee DF, Lemischka IR, Rendl M
  • Journal: Stem Cells 2011; (29):6 964-971
  • PubMed ID: 21563278

Citations & References

Human-induced pluripotent stem cells from blood cells of healthy donors and patients with acquired blood disorders.

  • Authors: Ye Z, Zhan H, Mali P, Dowey S, Williams DM, Jang YY, Dang CV, Spivak JL, Moliterno AR, Cheng L
  • Journal: Blood 2009 27:5473-5480
  • PubMed ID: 19797525
Catalog #

Citations & References

Establishment of induced pluripotent stem cells from aged mice using bone marrow-derived myeloid cells.

  • Authors: Cheng Z, Ito S, Nishio N, Xiao H, Zhang R, Suzuki H, Okawa Y, Murohara T, Isobe K
  • Journal: J Mol Cell Biol 2011; (3):2 91-98
  • PubMed ID: 21228011

Product FAQ

What is the recommended dispase (Dispase II, Cat. No. 17105-041) concentration to dislodge iPSC (induced pluripotent stem cells)? Is the working concentration indeed 2 mg/mL? This seems to be much higher when using the original dispase protocol that does not refer to the detachment of iPSC cells.

Answer

We typically make working solutions of 2 mg/mL, and we do not take the units per mg into account that differ from lot-to-lot. We are aware that different dispase batches may have different enzymatic activity but so far we have not encountered any problems using this method. It is important to not incubate the cells too long in dispase, this enzyme works quickly, and incubating too long can lead to clump sizes that are too small. So as long as you keep incubation to around 2-3 minutes, there should be no problem, even if the enzymatic activity may vary a bit from batch to batch.

The dispase manual does not specifically include information about its use with PSC (pluripotent stem cell) culture, so that is why there is a difference between the manual and the PSC protocol.

Answer Id: E12531

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Citations & References

Efficient generation of lens progenitor cells from cataract patient-specific induced pluripotent stem cells.

  • Authors: Qiu X, Yang J, Liu T, Jiang Y, Le Q, Lu Y
  • Journal: PLoS One 2012; (7):3 e32612-e32612
  • PubMed ID: 22403680

Citations & References

Limited gene expression variation in human embryonic stem cell and induced pluripotent stem cell-derived endothelial cells.

  • Authors: White MP, Rufaihah AJ, Liu L, Ghebremariam YT, Ivey KN, Cooke JP, Srivastava D
  • Journal: Stem Cells 2013; (31):1 92-103
  • PubMed ID: 23079999

Citations & References

Production of embryonic and fetal-like red blood cells from human induced pluripotent stem cells.

  • Authors: Chang CJ, Mitra K, Koya M, Velho M, Desprat R, Lenz J, Bouhassira EE
  • Journal: PLoS One 2011; (6):10 e25761-e25761
  • PubMed ID: 22022444

Citations & References

Somatic cells with a heavy mitochondrial DNA mutational load render induced pluripotent stem cells with distinct differentiation defects.

  • Authors: Wahlestedt M, Ameur A, Moraghebi R, Norddahl GL, Sten G, Woods NB, Bryder D,
  • Journal: Stem Cells (2014) 32:1173-1182
  • PubMed ID: 24446123

Citations & References

Human-induced pluripotent stem cells from blood cells of healthy donors and patients with acquired blood disorders

  • Authors: Ye, ZH; Zhan, HC; Mali, P; Dowey, S; Williams, DM; Jang, YY; Dang, CV; Spivak, JL; Moliterno, AR; Cheng, LZ
  • Journal: Blood 2009 27:5473-5480
Catalog #