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On-chip coupling of isoelectric focusing and free solution electrophoresis for multidimensional separations. Citations & References

  • Authors: Herr AE, Molho JI, Drouvalakis KA, Mikkelsen JC, Utz PJ, Santiago JG, Kenny TW
  • Journal: Anal Chem
  • PubMed ID: 12641239
Catalog #

What products do you offer for 2D gel electrophoresis of proteins? Product FAQ

Answer

We offer the following products for the first- and second-dimension separation of proteins:

First-dimension separation:

*Vertical gels for separation of proteins based on their isoelectric point (pI) (https://www.thermofisher.com/us/en/home/life-science/protein-expression-and-analysis/protein-gel-electrophoresis/protein-gels/specialized-protein-separation/isoelectric-focusing.html)

*Solution-phase isoelectric focusing of proteins using ZOOM Disks (immobilized buffer disks of specific pH) to reduce sample complexity, enrich low-abundance proteins, and increase the dynamic range of detection (https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/specialized-protein-gels/isoelectric-focusing/zoom-ief-fractionator.html)

*Mini gel system for high-throughput isoelectric focusing of proteins using ZOOM IPG (Immobilized pH Gradient) Strips (https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/specialized-protein-gels/2d-gel-electrophoresis/zoom-ipgrunner-system.html)

Second-dimension separation:

*ZOOM gels for 2D electrophoresis: NuPAGE Bis-Tris (Cat. No. NP0330BOX) and Tris-Glycine (Cat. No. EC60261BOX) mini gels with IPG wells ( to accommodate 7 cm ZOOM strips) for separation of proteins based on their molecular weight

Answer Id:: E10622

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A comparison of silver staining methods for detecting proteins in ultrathin polyacrylamide gels on support film after isoelectric focusing. Citations & References

  • Authors: Butcher LA, Tomkins JK
  • Journal: Anal Biochem
  • PubMed ID: 2415020

Integration of isoelectric focusing with parallel sodium dodecyl sulfate gel electrophoresis for multidimensional protein separations in a plastic microfluidic [correction of microfludic] network. Citations & References

  • Authors: Li Y, Buch JS, Rosenberger F, DeVoe DL, Lee CS
  • Journal: Anal Chem
  • PubMed ID: 14750871
Catalog #

Isoelectric focusing (IEF) and band detection with fluorogenic protease substrates. Citations & References

  • Authors: Sinha P, Gossrau R
  • Journal: Histochemistry
  • PubMed ID: 6149203

How does the ZOOM IPG Runner System work? Product FAQ

Answer

The ZOOM IPGRunner System provides a convenient and quick way to perform isoelectric focusing (IEF) of proteins in a vertical mini-gel format using immobilized pH gradient (IPG) strips for two- dimensional (2D) gel electrophoresis.

The major components of the ZOOM IPGRunner System are:

*ZOOM IPGRunner Mini-Cell (Cat. No. ZM0001)
*ZOOM IPGRunner Cassettes (1 pack of 10 cassettes, Cat. No. ZM0003)
*ZOOM Strips (12 ZOOM Strips, pH 3–10 NL, Cat. No. ZM0011)

Using the ZOOM Strips, proteins are separated based on their isoelectric point (pI). The proteins in the sample can be further separated in the second dimension, based on their molecular weight, by placing the IPG Strips into the IPG well of a NuPAGE Bis-Tris ZOOM gel or Tris-Glycine ZOOM gel. The proteins separated in the second dimension can be visualized as spots by the use of SimplyBlue SafeStain or silver staining, or blotted onto membranes. Protein spots can be also excised from the gel or the membranes to be further analyzed by mass spectrometry or chemical microsequencing to facilitate protein identification.

Answer Id:: E10669

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Dynamic isoelectric focusing for proteomics. Citations & References

  • Authors: Montgomery R, Jia X, Tolley L
  • Journal: Anal Chem
  • PubMed ID: 16970328
Catalog #

Two-dimensional polyacrylamide gel electrophoresis of proteins labeled with the fluorophore monobromobimane prior to first-dimensional isoelectric focusing: imaging of the fluorescent protein spot patterns using a cooled charge-coupled device. Citations & References

  • Authors: Urwin VE, Jackson P
  • Journal: Anal Biochem
  • PubMed ID: 8465962
Catalog #

How are proteins separated on an IEF gel? Product FAQ

Answer

Isoelectric focusing (IEF) is an electrophoresis technique that separates proteins on the basis of their isoelectric point (pI). The pI is the pH at which a protein has no net charge and does not move in an electric field. IEF gels effectively create a pH gradient so proteins separate according to their unique pI.

Answer Id:: E10627

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A multiple high-resolution mini two-dimensional polyacrylamide gel electrophoresis system: imaging two-dimensional gels using a cooled charge-coupled device after staining with silver or labeling with fluorophore. Citations & References

  • Authors: Urwin VE, Jackson P
  • Journal: Anal Biochem
  • PubMed ID: 1716069
Catalog # D16

Fluorescence imaging detection for capillary isoelectric focusing. Citations & References

  • Authors: Wu XZ, Wu J, Pawliszyn J
  • Journal: Electrophoresis
  • PubMed ID: 8529617
Catalog # C2990(Discontinued)

How does the ZOOM IEF Fractionator system work? Product FAQ

Answer

The ZOOM IEF Fractionator is designed to fractionate samples on the basis of Isoelectric point prior to 2D Electrophoresis, 1D Electrophoresis, or 2D Liquid Chromatography/Mass Spectrometry analysis.

The main components of the ZOOM IEF Fractionator system are the ZOOM IEF Fractionator, ZOOM Disks, and ZOOM Reagents. Fractionation in the ZOOM IEF Fractionator is performed using a series of Sample Chambers connected in tandem and separated by thin membranes (ZOOM Disks) containing covalently attached buffers of defined pH. The protein sample is loaded into multiple Sample Chambers separated by these disks or a combination of disks and spacers and subjected to solution-phase IEF for 3 hours. At the end of solution-phase IEF, the protein sample is separated into well-resolved fractions based on the pI of each protein. Samples can be resolved into as many as six fractions (using seven disks of specific pH) from pH 3 to pH 12.

Answer Id:: E10653

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Rapid imaging, using a cooled charge-coupled-device, of fluorescent two-dimensional polyacrylamide gels produced by labelling proteins in the first-dimensional isoelectric focusing gel with the fluorophore 2-methoxy-2,4-diphenyl-3(2H)furanone. Citations & References

  • Authors: Jackson P, Urwin VE, Mackay CD
  • Journal: Electrophoresis
  • PubMed ID: 2466662
Catalog # B153

What is 2D protein gel electrophoresis? Product FAQ

Answer

2D protein gel electrophoresis is the separation of proteins in two dimensions. In the first dimension, proteins are separated by their isoelectric point (pI) using isoelectric focusing, and in the second dimension, they are separated by their mass using SDS-PAGE.

Answer Id:: E10621

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Lsoelectric focusing phenotyping and denaturing gradient gel electrophoresis genotyping: a comparison of two methods in detection of alpha-1-antitrypsin variants Citations & References

  • Authors: Ljujic, M; Topic, A; Divac, A; Nikolic, A; Petrovic-Stanojevic, N; Surlan, M; Mitic-Milikic, M; Radojkovic, D
  • Journal: Translational Research
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