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JAK1 Recombinant Human Protein

Janus kinase 1 (JAK1), is a protein tyrosine kinase that is involved in the interferon-alpha⁄beta and -gamma signal transduction pathways.

Phospho-JAK1 (Tyr1022, Tyr1023) Antibody (59H4L5), ABfinity™ Rabbit Monoclonal (Invitrogen™)

Phospho-JAK1 (Tyr1022, Tyr1023) Monoclonal Antibody for Western Blot, IF, ICC, ChIP

Phospho-JAK1 (Tyr1022, Tyr1023) Polyclonal Antibody (Invitrogen™)

Phospho-JAK1 (Tyr1022, Tyr1023) Polyclonal Antibody for Western Blot, IF, ICC

Phospho-JAK1 (Tyr1022) Polyclonal Antibody (Invitrogen™)

Phospho-JAK1 (Tyr1022) Polyclonal Antibody for Western Blot, IHC (P)

JAK1 Polyclonal Antibody (Invitrogen™)

JAK1 Polyclonal Antibody for IHC (P)

Phospho-JAK1 (Tyr1022) Polyclonal Antibody (Invitrogen™)

Phospho-JAK1 (Tyr1022) Polyclonal Antibody for Western Blot, IHC (P)

JAK1 Polyclonal Antibody (Invitrogen™)

JAK1 Polyclonal Antibody for Western Blot, IF, ICC

Phospho-JAK1 (Tyr1022, Tyr1023) Polyclonal Antibody (Invitrogen™)

Phospho-JAK1 (Tyr1022, Tyr1023) Polyclonal Antibody for Western Blot

JAK1 Polyclonal Antibody (Invitrogen™)

JAK1 Polyclonal Antibody for Western Blot

JAK1 Monoclonal Antibody (JM75-03) (Invitrogen™)

JAK1 Monoclonal Antibody for Western Blot, IHC, Flow

JAK1 Polyclonal Antibody (Invitrogen™)

JAK1 Polyclonal Antibody for Western Blot

JAK1 Polyclonal Antibody (Invitrogen™)

JAK1 Polyclonal Antibody for Western Blot

CellSensor™ ISRE-bla Jurkat Cell Line

The CellSensor® ISRE-bla Jurkat cell line contains a beta-lactamase reporter gene under control of the interferon-stimulated response element (ISRE) stably integrated into Jurkat cells. To construct this cell line, the ISRE-bla construct was transduced into Jurkat cells by lentivirus. Flow cytometry was used to isolate cells responsive to IFNα. This cell line was validated for DMSO tolerance, cell number, stimulation time, substrate loading time, as well as ' and EC50 concentrations of IFN&alpha. The CellSensor® ISRE-bla Jurkat cell line is responsive to Interferon alpha (IFN-alpha) and Interferon beta and can be used to probe the type I interferon-induced JAK-STAT signaling pathways. This cell line can be adapted for high-throughput screening of agonist or antagonist compound libraries. Candidate drugs can also be tested for dose response against this cell line. ISRE-bla Jurkat cells were treated with agonist IFN-alpha; over the indicated concentration range in a 384-well format. Cells were incubated for 5 hours with agonist and 0.5% DMSO and then combined with LiveBLAzer™-FRET B/G Substrate (CCF4-AM) for 2 hours. Fluorescence emission values at 460 nm and 530 nm were obtained using a standard fluorescence plate reader, and the 460/530 ratios were plotted against the concentration of the agonist. Academic and non-profit customers, please inquire for special pricing.

CellSensor™ GAS-bla ME-180 Cell Line

The CellSensor® GAS-bla ME-180 cell line contains a beta-lactamase reporter gene under control of the Gamma Activated Sequence (GAS) response element stably integrated into ME-180 cells. To construct this cell line, the GAS-bla construct was transduced into ME-180 cells by lentivirus. Flow cytometry was used to isolate cells responsive to Interferon Gamma (IFN-γ). This cell line is validated for DMSO tolerance, cell number, stimulation time, substrate loading time, as well as Z'and EC50 concentrations for IFN-&gamma. The CellSensor® GAS-bla ME-180 cell line is responsive to IFN-γ and can be used to probe the JAK/STAT signaling pathway. This cell line can be adapted for high-throughput screening of agonist or antagonist compound libraries. Candidate drugs can be tested for dose response against this cell line. Academic and non-profit customers, please inquire for special pricing.

CellSensor™ ISRE-bla HEK293T Cell Line

Interferon cell signaling is a critical pathway involved in viral defense and autoimmune disease. The CellSensor® ISRE-bla HEK 293T cell line is responsive to interferon alpha and can be used to probe the JAK/STAT signaling pathway.The CellSensor™ ISRE-bla HEK 293T cell line contains the β-lactamase reporter gene under the control of the interferon stimulated response element (ISRE). This cell line was engineered by transduction of the ISRE-bla construct into HEK 293T cells by lentivirus. Flow cytometry was then used to isolate a pool of clones responsive to interferon alpha a stimulation. A dose response curve for this assay against interferon alpha is available in the validation packet. Academic and non-profit customers, please inquire for special pricing.