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LanthaScreen™ c-Jun (1-79) HeLa Cell Line

The LanthaScreen®c-Jun (1-79) HeLa cell line contains a fusion protein consisting of GFP and a fragment encoding for AA 1-79 of c-Jun under the control of a CMV promoter stably transfected into HeLa cell lines. HeLa is a cervical cancer cell line which shows inducible activation of JNK by a number of different ligands, such as TNF-a and EGF, which leads to the transient phosphorylation of GFP-c-Jun (1-79). This LanthaScreen®cell line therefore allows the analysis of JNK activity using GFP-c-Jun phosphorylation as readout. The GFP-c-Jun (1-79) construct was transfected into HeLa cells using Lipofectamine™2000, followed by selection with Blasticidin. This cell line is a clonal population isolated by flow cytometry using GFP fluorescence as sorting marker. This cell line is validated for EC50 and Z' under optimized conditions using TNF-a as ligand for JNK mediated GFP-c-Jun (1-79) phosphorylation. This cell lines has also been tested for assay performance under variable experimental conditions, including cell plating density, stimulation time, DMSO tolerance and assay development time. Additional information using alternate stimuli is also available. Academic and non-profit customers, please inquire for special pricing.

c-Jun Antibody (2HCLC), ABfinity™ Rabbit Oligoclonal (Invitrogen™)

c-Jun Oligoclonal Antibody for Western Blot, IF, ICC, ChIP

c-Jun (Total) Multispecies InstantOne™ ELISA Kit (Invitrogen™)

c-Jun (Total) Multispecies InstantOne™ ELISA Kit for ELISA

Phospho-c-Jun (Thr91, Thr93, Thr95) Antibody (13HCLC), ABfinity™ Rabbit Oligoclonal (Invitrogen™)

Phospho-c-Jun (Thr91, Thr93, Thr95) Oligoclonal Antibody for Western Blot, ChIP

JUN™ Spectral Calibration Plate, 96-well (Applied Biosystems™)

This plate is used for JUN® spectral calibration of your Applied Biosystems® 7500 or ViiA™ 7 real-time PCR system with a 96-well block. Please see your instrument maintenance guide for full instructions on use. The dye formulations will help improve multiplexing results by more accurately representing fluorescent spectra that are part of your real-time PCR experiments.

c-Jun (Phospho) [pS73] Multispecies InstantOne™ ELISA Kit (Invitrogen™)

c-Jun (Phospho) [pS73] Multispecies InstantOne™ ELISA Kit for ELISA

Phospho-c-Jun (Ser73) Polyclonal Antibody (Invitrogen™)

Phospho-c-Jun (Ser73) Polyclonal Antibody for Western Blot, IF, ICC

LanthaScreen™ Tb-p-c-jun (pSer73) Antibody Kit

A part of the Activating protein-1 (AP-1) transciption factor, c-Jun is phosphorylated at Ser73 by members of the JNK family.

For use in combination with the GFP-c-Jun physiological protein substrate (PV4446) to evaluate inhibitors in medium to high throughput screening applications.

c-Jun Antibody (2H4L1), ABfinity™ Rabbit Monoclonal (Invitrogen™)

c-Jun Monoclonal Antibody for Western Blot, IF, ICC, ChIP

LanthaScreen™ Tb-p-c-jun (pSer73) Antibody

A part of the activating protein-1 (AP-1) transcription factor, c-Jun is phosphorylated at Ser73 by members of the JNK family.

For use in combination with the GFP-c-Jun physiological protein substrate (PV4446) to evaluate inhibitors in medium to high throughput screening applications.

TaqMan™ GAPDH Assay, JUN™ dye/QSY™ probe (Applied Biosystems™)

The Applied Biosystems® TaqMan® GAPDH Assay with JUN® dye-labeled QSY® probe provides a pre-formulated assay for quantitating human GAPDH. This assay enables relative gene expression quantification in cDNA samples when used with other gene expression assays. The assay consists of a JUN® dye-labeled QSY® probe plus sequence-specific forward and reverse primers, and it can be used for multiplex or singleplex PCR reactions.

Benefits of this assay include:

• Eliminate assay design time by using pre-designed primers and pre-designed JUN® dye-labeled TaqMan® probe
• Minimize experimental optimization time
• Use in conjunction with FAM™ or VIC® dye-labeled predesigned gene expression assays

Save time in assay design, formulation, and testing by using the TaqMan® GAPDH Assay as your control when quantifying gene expression. This pre-designed probe and primer set enables you to normalize the amount of sample RNA or DNA in a reaction when used with a control sample. Or use it for relative gene expression quantification in cDNA samples when used with other gene expression assays.

The JUN® dye is optimized for use with the 4th filter of our QuantStudio™, ViiA™ 7, and 7500 series real-time PCR instruments. Please note that master mix containing ROX™ dye cannot be used with this assay (with JUN® dye) due to an overlap of spectra. TaqMan® Multiplex Master Mix with MUSTANG PURPLE® dye is recommended.

c-Jun (Total/Phospho) Multispecies InstantOne™ ELISA Kit (Invitrogen™)

c-Jun (Total/Phospho) Multispecies InstantOne™ ELISA Kit for ELISA

JUN™ Dye Spectral Calibration Plate for Multiplex qPCR, Fast 96-well (Applied Biosystems™)

This plate is used for JUN® dye spectral calibration of your 7500 Fast, ViiA™ 7, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, and QuantStudio™ 12K Flex real-time PCR systems with a Fast 96-well block. Calibration and verification should be run at least every six months. Please see your instrument maintenance guide for full instructions on use. The dye formulations will improve results with multiplexing by more accurately representing fluorescent spectra found in your real-time PCR experiments.

JUN™ Dye Spectral Calibration Plate for Multiplex qPCR, 384-well (Applied Biosystems™)

This plate is used for JUN® dye spectral calibration of your ViiA™ 7, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, and QuantStudio™ 12K Flex real-time PCR systems with a 384-well block. Calibration and verification should be run at least every six months. Please see your instrument maintenance guide for full instructions on use. The dye formulations will improve results with multiplexing by more accurately representing fluorescent spectra found in your real-time PCR experiments.

QuantStudio™ 3/5 Spectral Calibration Plate 2 (ABY™, JUN™, MUSTANG PURPLE™ dyes), 96-well, 0.2 mL (Applied Biosystems™)

This plate establishes the pure dye spectra and multicomponent values for ABY™, JUN™, and MUSTANG PURPLE™ dyes on Applied Biosystems™ QuantStudio™ 3 and 5 96-well 0.2-mL real-time PCR systems. It enables simultaneous multiple dye calibrations in one plate, significantly reducing effort and time required for dye calibration. In addition, the formulations of the dyes in this plate improve results with multiplexing by more accurately representing the fluorescent spectra of the respective probes in your real-time PCR experiments. Please refer to the instrument user guide for full instructions on usage.