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CD309 (FLK1) Monoclonal Antibody (Avas12a1), Biotin, eBioscience™ (Invitrogen™)

CD309 (FLK1) Monoclonal Antibody for Flow

CD309 (FLK1) Monoclonal Antibody (Avas12a1), APC, eBioscience™ (Invitrogen™)

CD309 (FLK1) Monoclonal Antibody for Flow

CD309 (FLK1) Monoclonal Antibody (Avas12a1), Alexa Fluor 700, eBioscience™ (Invitrogen™)

CD309 (FLK1) Monoclonal Antibody for Flow

CD309 (FLK1) Monoclonal Antibody (Avas12a1), Super Bright 600, eBioscience™ (Invitrogen™)

CD309 (FLK1) Monoclonal Antibody for Flow

CD309 (FLK1) Monoclonal Antibody (Avas12a1), Super Bright 436, eBioscience™ (Invitrogen™)

CD309 (FLK1) Monoclonal Antibody for Flow

CD309 (FLK1) Monoclonal Antibody (Avas12a1), PE, eBioscience™ (Invitrogen™)

CD309 (FLK1) Monoclonal Antibody for Flow

CD309 (FLK1) Monoclonal Antibody (Avas12a1), eBioscience™ (Invitrogen™)

CD309 (FLK1) Monoclonal Antibody for Western Blot, IHC, Flow

KDR Monoclonal Antibody (OTI2A1), TrueMAB™ (OriGene)

KDR Monoclonal Antibody for Western blot

KDR Monoclonal Antibody (OTI2D7), TrueMAB™ (OriGene)

KDR Monoclonal Antibody for Western blot

KDR Monoclonal Antibody (OTI12C1), TrueMAB™ (OriGene)

KDR Monoclonal Antibody for Western blot

KDR Monoclonal Antibody (OTI12C1), TrueMAB™ (OriGene)

KDR Monoclonal Antibody for Western blot

KDR Monoclonal Antibody (OTI2A1), TrueMAB™ (OriGene)

KDR Monoclonal Antibody for Western blot

KDR Monoclonal Antibody (OTI2D7), TrueMAB™ (OriGene)

KDR Monoclonal Antibody for Western blot

CD309 (FLK1) Recombinant Rabbit Monoclonal Antibody (SU03-42) (Invitrogen™)

CD309 (FLK1) Recombinant Monoclonal Antibody for Western Blot, IF, ICC, IHC

VEGFR-1/FLT-1 Polyclonal Antibody (Proteintech)

VEGFR-1/FLT-1 Polyclonal Antibody for Western Blot, IF, ICC, Flow, IP

VEGF Receptor 2/KDR Human ELISA Kit (Invitrogen™)

VEGF Receptor 2/KDR Human ELISA Kit for ELISA

KDR (VEGFR2) Recombinant Human Protein

KDR (VEGFR2) is a type III receptor tyrosine kinase. It binds vascular endothelial growth factor with high affinity in vitro, and is expressed early in development by endothelial cell precursors.

VEGF-R2 Human ProcartaPlex™ Simplex Kit (Invitrogen™)

The Human VEGF-R2 Simplex ProcartaPlex kit measures VEGF-R2 protein and is designed to be combinable with other Simplex kits so that you can create your own multiplex panel that utilizes Luminex xMAP technology for protein detection/quantitation. When combining multiple Simplex kits (i.e., when you are not using a pre-configured Multiplex Panel), only one buffer kit (sold separately) is needed for each assay plate regardless of plex size.

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate up to 80 protein targets simultaneously when using the FLEXMAP 3D or Luminex 200 instrument and up to 50 protein targets when using the MAGPIX instrument. ProcartaPlex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

Flexible panels—design your own panels with Simplex kits to measure your own array of targets
More results per sample—measure up to 80 protein targets in a single 25–50 µL sample
Well-established Luminex technology—the most referenced multiplexing platform for protein detection and quantitation

The Luminex MagPlex superparamagnetic microsphere beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create 100 spectrally unique signatures that can be identified by the Luminex xMAP detec¬tion systems (Luminex 200, FLEXMAP 3D, and MAGPIX systems). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a ProcartaPlex multiplex assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a magnetic bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile up to 80 times more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex Simplex kits provide the ability to create your own unique panel . More than 90% of ProcartaPlex Simplex targets can be combined, providing you with superior flexibility when creating your own multiplex panel.

ProcartaPlex Simplex kits are available across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information, including a comprehensive list of individual protein targets.

Reactivity/Species
Human

Suitable Sample Types
cell culture supernatant, serum, plasma

Sample Volume
Serum, Plasma: 25 µL; CCS: 50 µL

Reported Application
Multiplex Immunoassay

VEGF Receptor 2 Monoclonal Antibody (KDR-2 or 260.4), Biotin (Invitrogen™)

VEGF Receptor 2 Monoclonal Antibody for Western Blot, Flow

VEGF Receptor 2 Monoclonal Antibody (KDR-2 or 260.4) (Invitrogen™)

VEGF Receptor 2 Monoclonal Antibody for Western Blot, IHC, ELISA

VEGF Receptor 2 (VEGF R2/VEGFA) Mouse ELISA Kit (Invitrogen™)

VEGF Receptor 2 (VEGF R2/VEGFA) Mouse ELISA Kit for ELISA

AffiniPure F(ab')2 Fragment Ra (JAC-309-006-043)

AffiniPure F(ab')2 Fragment Rabbit Anti-Human IgM, Fc5µ Fragment Specific

Tris(4,7-diphenyl-1,10-phenant (CAY-16117-1mg)

Tris(4,7-diphenyl-1,10-phenanthroline)ruthenium II dichloride complex