Product FAQ

Are the Keratinocyte SFM basal medium and supplement kit available separately?

Answer

The basal medium is not available for purchase individually, and can only be ordered as part of the Keratinocyte SFM kit (catalog # 17005-042). The supplements are available separately: Bovine Pituitary Extract is catalog # 13028-014 and the EGF is catalog # PHG0311.

Answer Id: E4742

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Product FAQ

For how long can I store Keratinocyte-SFM at 4 degrees C?

Answer

The base Keratinocyte-SFM has a shelf life of 12 months when stored at 2 to 8 degrees C, protected from light. Keratinocyte-SFM Supplement has a shelf life of 18 months when stored at -20 to 5 degrees C. The complete media should not be stored for longer than 4 weeks at 4 degrees C

Answer Id: E17484

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Manual / Product Insert

Keratinocyte SFM

Version: MAN0006271 3.00 (20Jan2014)

Product FAQ

Are the Defined Keratinocyte SFM basal medium and supplement available separately?

Answer

The Defined Keratinocyte SFM basal medium and supplement are not available separately. The kit can be ordered with catalog #10744-019.

Answer Id: E5556

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Manual / Product Insert

Defined Keratinocyte-SFM (1X), liquid

Version: MAN0007310 1.01 (15 Jul 13)
Catalog #

Product FAQ

What media do you recommend for culturing Human Corneal Epithelial Cells (HCEC)?

Answer

We recommend culturing HCECs in keratinocyte serum-free medium (KSFM), as the cells are manufactured and quality-controlled using this medium. However, if a defined growth system is preferred, we recommend defined keratinocyte serum-free medium (Defined Keratinocyte-SFM). Note that a Coating Matrix Kit is required for culturing HCECs in Defined Keratinocyte-SFM. In-house testing has shown comparable HCEC growth and morphology in both KSFM and Defined Keratinocyte-SFM. Please note that these cells have been discontinued.

Answer Id: E11974

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Product FAQ

Which serum-free media can be used to culture human keratinocytes?

Answer

There are a variety of options for serum-free culture of human keratinocytes. Keratinocyte SFM (catalog # 17005-042) is a widely used kit that includes the basal medium, bovine pituitary extract (BPE) and Epidermal Growth Factor (EGF). If you would like a serum-free, defined culture system, the options are the Defined Keratinocyte SFM kit (catalog #10744-019) or Epilife medium (catalog # M-EPI-500-CA) supplemented with EDGS (catalog # S-012-5). If you would like an animal origin free defined system, Epilife medium (catalog # M-EPI-500-CA) with S7 supplement (catalog # S-017-5) is an excellent choice.

Answer Id: E4740

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Product FAQ

Do Human Corneal Epithelial Cells (HCEC) require feeder cells or matrix coatings for proliferation?

Answer

Feeder cells are not needed for culturing HCECs using KSFM or Defined Keratinocyte-SFM. However, HCECs cultured in Defined Keratinocyte-SFM require the use of a Coating Matrix Kit (recombinant type I collagen) for efficient attachment and growth. Please note that these cells have been discontinued.

Answer Id: E11975

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Product FAQ

Which serum-free media can be used to culture mouse keratinocytes?

Answer

The Keratinocyte SFM without calcium chloride (catalog # 37010-022) is appropriate for the culture of mouse keratinocytes. The medium should be supplemented with calcium chloride at a final concentration of 0.02 mM.

Answer Id: E4741

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Product FAQ

What is the expected growth rate for Human Corneal Epithelial Cells (HCEC)?

Answer

When cultured under recommended conditions, HCECs grown in KSFM typically achieve growth rates of ~0.75-1.0 population doublings per day. Similar growth rates are seen using Defined Keratinocyte-SFM. Please note that these cells have been discontinued.

Answer Id: E11976

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Citations & References

Culture and characterization of human junctional epithelial cells.

  • Authors: Matsuyama T; Izumi Y; Sueda T;
  • Journal: J Periodontol (1997) 68:229-239
  • PubMed ID: 9100198
Catalog #

Product Literature

Brochure: Human primary cell culture sourcebook

Product Literature

Brochure: 3D cell culture product selection guide for organoids and spheroids

Product Literature

Specialty and Serum-Free Media

Product Literature

Serum-Free Cell Culture