Citations & References

Serum amyloid A3 binds MD-2 to activate p38 and NF-?B pathways in a MyD88-dependent manner.

  • Authors: Deguchi A, Tomita T, Omori T, Komatsu A, Ohto U, Takahashi S, Tanimura N, Akashi-Takamura S, Miyake K, Maru Y,
  • Journal: J Immunol (2013) 191:1856-1864
  • PubMed ID: 23858030
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Citations & References

Mouse marginal zone B cells harbor specificities similar to human broadly neutralizing HIV antibodies.

  • Authors: Pujanauski LM, Janoff EN, McCarter MD, Pelanda R, Torres RM,
  • Journal: Proc Natl Acad Sci U S A (2013) 110:1422-1427
  • PubMed ID: 23288906
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Manual / Product Insert

Alexa Fluor 660 Protein Labeling Kit

Version: 08-24-2004
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Limited Use Label License

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Limited Use Label License
358
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Product FAQ

Do you have any tips on using the ULYSIS Nucleic Acid Labeling Kits for RNA labeling?

Answer

A preliminary protocol modifies our DNA-labeling protocol: Do not nuclease-treat the RNA, but label it directly by incubating for 10 minutes at 90°C or 15 minutes at 85°C. Add 2 µg of glycogen for every 1 µg of RNA and purify by ethanol precipitation. Refer to these publications:

- Babak T, Zhang W, Marros Q et al. (2004) Probing microRNAs with microarrays: tissue specificity and functional inference. RNA 10(11):1813-1819.
- Hiley SL, Jackman J, Babak T et al. (2005) Detection and discovery of RNA modifications using microarrays. Nucleic Acids Res 33(1):e2.
- Torchet C, Badis G, Devaux F et al. (2005) The complete set of H/ACA snoRNAs that guide rRNA pseudouridylations in Saccharomyces cerevisiae. RNA 11(6):928-938.

Answer Id: E8095

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