Product Literature

Application note - An approach for analyzing genetic heterogeneity in retrospective tumor samples using laser capture microdissection, real-time PCR, and next-generation sequencing

Product FAQ

I want to isolate miRNA from formalin-fixed and unfixed laser capture microdissection (LCM) samples. Which kit will be the best for this?

Answer

For recovery of miRNA from formalin-fixed samples, we recommend using RecoverAll Total Nucleic Acid Isolation Kit for FFPE. You can isolate total and miRNA using the RecoverAll Total Nucleic Acid Isolation Kit for FFPE, then use that prep for enrichment of miRNA using the enrichment protocol described in the instructions for the mirVana kit. For unfixed LCM samples, you could use an RNAqueous kit.

Answer Id: E15461

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Citations & References

Gene Expression Analysis of Immunostained Endothelial Cells Isolated From Formaldehyde-Fixated Paraffin Embedded Tumors Using Laser Capture Microdissection-A Technical Report

  • Authors: Kaneko, T; Okiji, T; Kaneko, R; Suda, H; Noer, JE
  • Journal: MICROSCOPY RESEARCH AND TECHNIQUE 2009 12:908-912

Product Literature

Brochures & Specifications: ArcturusXT Laser Capture Microdissection System Product Brochure

Manual / Product Insert

Quick Ref: Laser Capture Microdissection Cells-to-CT Kit

Version: MAN0010915 Rev. A (02Oct2014)

Product Literature

Fungal DNA sequencing from laser capture microdissection samples

Manual / Product Insert

User Guide: Arcturus XT Laser Capture Microdissection (LCM) System

Version: MAN0016091 Rev. A.0 (22Sep2016)

Product FAQ

Which RNA isolation kit do you recommend using with Arcturus Laser Capture Microdissection (LCM) samples?

Answer

The Arcturus PicoPure RNA Isolation Kit (Cat. Nos. KIT0204, KIT0214) is specifically designed to consistently recover high-quality total RNA from fewer than 10 cells, even from a single cell. This kit is designed to provide consistent, efficient RNA recovery without sacrificing quality. Small volume elution allows you to maximize your recovery of RNA from small numbers of cells for use with your gene expression analysis experiments.

For LCM cells from paraffin sections, we recommend using the Arcturus Paradise Plus RNA Extraction and Isolation Kit (Cat. No. KIT0312I) that unlocks RNA from formalin-fixed, paraffin-embedded (FFPE) tissues. As little as 5 ng of the isolated fixed RNA can then be amplified using reagents and methods that have been specially optimized to overcome the challenges of cross-linked FFPE templates.

We also recommend the Arcturus Paradise PLUS QC Kit (Cat. Nos. KIT0303, KIT0313). This kit offers a simple solution for evaluating the quality of RNA from formalin-fixed paraffin-embedded (FFPE) tissues before proceeding with LCM and downstream molecular analysis. The reagents provided in the kit are optimized to enable efficient extraction, isolation, and reverse transcription of total RNA from FFPE tissue scrapes. The RNA quantity and quality is then measured using quantitative real-time PCR with a housekeeping gene like beta actin.

Answer Id: E15454

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Citations & References

Simultaneous isolation of DNA and RNA from the same cell population obtained by laser capture microdissection for genome and transcriptome profiling.

  • Authors: Xu C, Houck JR, Fan W, Wang P, Chen Y, Upton M, Futran ND, Schwartz SM, Zhao LP, Chen C, Mendez E
  • Journal: J Mol Diagn 2008; (10):2 129-134
  • PubMed ID: 18258925

Product Literature

Application Note: Gene expression profiling of a single cell using laser capture microdissection and OpenArray technology

Product FAQ

I want to isolate miRNA from formalin-fixed and unfixed laser capture microdissection (LCM) samples. Which kit will be best for this?

Answer

For recovery of miRNA of formalin-fixed samples, we recommend using RecoverAll Total Nucleic Acid Isolation Kit for FFPE. You can isolate total and miRNA using the RecoverAll Total Nucleic Acid Isolation Kit for FFPE, then use that prep for enrichment of miRNA using the enrichment protocol described in the instructions for the mirVana kit. For unfixed LCM samples, you could use an RNAqueous kit.

Answer Id: E7887

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Citations & References

Optimization of laser capture microdissection and RNA amplification for gene expression profiling of prostate cancer.

  • Authors: Kube DM, Savci-Heijink CD, Lamblin AF, Kosari F, Vasmatzis G, Cheville JC, Connelly DP, Klee GG,
  • Journal: BMC Mol Biol (2007) 8:25-25
  • PubMed ID: 17376245

Product FAQ

What kit do you recommend using for DNA isolation from Arcturus Laser Capture Microdissection (LCM) samples?

Answer

The Arcturus PicoPure DNA Extraction Kit (Cat. No. KIT0103) offers an easy, streamlined genomic extraction procedure that produces PCR-ready DNA. It allows you to extract and amplify DNA in the same tube, without organic extraction or spin columns, and to recover DNA from as few as 10 cells prepared by laser capture microdissection (LCM) or other tissues. The kit provides conveniently packaged, stable Proteinase K, PCR-compatible DNA Reconstitution Buffer and a complete user guide. 

Answer Id: E15456

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