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Where do I find buffer recipes for your precast protein gels? Product FAQ

Answer

The formulations of buffers for our precast protein gels can be found at this link: https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-electrophoresis-buffers-reagents.html

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Answer Id: E4465

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What products do you offer for 2D gel electrophoresis of proteins? Product FAQ

Answer

We offer the following products for the first- and second-dimension separation of proteins:

First-dimension separation:

*Vertical gels for separation of proteins based on their isoelectric point (pI) (https://www.thermofisher.com/us/en/home/life-science/protein-expression-and-analysis/protein-gel-electrophoresis/protein-gels/specialized-protein-separation/isoelectric-focusing.html)

*Solution-phase isoelectric focusing of proteins using ZOOM Disks (immobilized buffer disks of specific pH) to reduce sample complexity, enrich low-abundance proteins, and increase the dynamic range of detection (https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/specialized-protein-gels/isoelectric-focusing/zoom-ief-fractionator.html)

*Mini gel system for high-throughput isoelectric focusing of proteins using ZOOM IPG (Immobilized pH Gradient) Strips (https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/specialized-protein-gels/2d-gel-electrophoresis/zoom-ipgrunner-system.html)

Second-dimension separation:

*ZOOM gels for 2D electrophoresis: NuPAGE Bis-Tris (Cat. No. NP0330BOX) and Tris-Glycine (Cat. No. EC60261BOX) mini gels with IPG wells ( to accommodate 7 cm ZOOM strips) for separation of proteins based on their molecular weight

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Answer Id: E10622

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What lysis buffers do you offer for mammalian cells? Product FAQ

Answer

Please see the various lysis buffers we offer (https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-purification-isolation/cell-lysis-fractionation/cell-lysis-total-protein-extraction.html) for total protein extraction from mammalian cells.

Find additional tips, troubleshooting help, and resources within our Cell Lysis and Fractionation Support Center.

Answer Id: E12798

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I have spilled my ProcartaPlex assay buffer, can I purchase more? Product FAQ

Answer

Yes, our Universal Assay Buffer (Cat. No. EPX-11110-000) can be bought separately. We offer most of our ProcartaPlex buffers and reagents as stand-alone items. A complete list of what is currently available can be found on our website here, under "Accessories": https://www.thermofisher.com/us/en/home/life-science/antibodies/immunoassays/procartaplex-assays-luminex/procartaplex-immunoassays/procartaplex-preconfigured-panels.html.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Answer Id: E16832

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Is milk a good blocking reagent to use with SuperSignal West Pico PLUS Chemiluminescent Substrate? Product FAQ

Answer

Yes and no. Milk contains variable amounts of biotin so it should not be used with avidin/biotin detection systems. Milk also contains varying amounts of phosphoproteins that may make interfere with anti-phosphotyrosine procedures. A variety of blocking buffers are compatible with the substrate. Please see our Blocking Buffer selection table (https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/western-blot-buffers/blocking-buffers-western-blotting.html#table).

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Answer Id: E8521

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Is milk a good blocking reagent to use with SuperSignal West Dura Extended Duration Substrate? Product FAQ

Answer

Yes and no. Milk contains variable amounts of biotin so it should not be used with avidin/biotin detection systems. Milk also contains varying amounts of phosphoproteins that may make interfere with anti-phosphotyrosine procedures. A variety of blocking buffers are compatible with the substrate. Please see our Blocking Buffer selection table (https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/western-blot-buffers/blocking-buffers-western-blotting.html#table).

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Answer Id: E8497

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What is the difference between the GeneArt Seamless PLUS Cloning and Assembly Kit and the original GeneArt Seamless Cloning and Assembly Kit? Product FAQ

Answer

The GeneArt Seamless PLUS kit is an improved version of the original kit. It is recommended for the assembly of up to 4 fragments and a vector totaling up to 40 kb compared to 13 kb for the original. The GeneArt enzyme mix is also provided as a 2X mix with buffer that can be stored at -20 degrees C instead of -80 degrees C. Finally, the kit comes with the linearized pYES7L vector and Stbl3/pRK2013 cells that allows for horizontal transfer of the construct into a variety of recipient strains. To select the GeneArt Seamless Cloning and Assembly kit that is best for your application, please visit https://www.thermofisher.com/us/en/home/life-science/cloning/seamless-cloning-and-genetic-assembly.html.

Answer Id: E6822

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What thermal stable DNA polymerase is recommended for PCR amplification of long PCR targets? Product FAQ

Answer

Successful amplification of long PCR targets is dependent on variables such as sufficient extension time during the PCR amplification, cosolvent addition, pH of the reaction buffer, salt concentration, primer design, use of a hot start, DNA sample integrity, and the enzyme's proofreading and polymerase activities. A few examples of our long PCR enzymes include our Elonagase enzyme mix that can be used for amplicons up to 30kb (blend of Taq and proofreading enzyme) or our Phire Hot Start II enzyme mix that can be used for amplicons up to 20 kb (Taq polymerase). Read more here: https://www.thermofisher.com/us/en/home/life-science/pcr/pcr-enzymes-master-mixes/long-fragment-pcr.html

Answer Id: E1083

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Will glycerol pose a problem when purifying antibodies with the Pierce Antibody Clean-up Kit (Cat. No. 44600)? Product FAQ

Answer

When using the Pierce Antibody Clean-up Kit, glycerol may pose a problem for the spin desalting columns for buffer exchange, but not for the Melon Gel itself. If glycerol concentrations are high, the solution becomes viscous enough to exert a frictional drag during spin column chromatography and the performance and yield may suffer. Please find a technical tip on using Melon Gel for cleaning up BSA or gelatin from commercial antibodies.

If the glycerol concentration is higher than 10%, dialysis may be considered to exchange the sample into the Melon Gel buffer. We have some dialysis devices suitable for small volume dialysis, e.g., the Slide-A-Lyzer MINI devices: see here. Alternatively, some of the smaller Slide-A-Lyzer dialysis cassettes can be used: see here.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Answer Id: E21533

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Is milk a good blocking reagent to use with SuperSignal West Femto Maximum Sensitivity Substrate? Product FAQ

Answer

Yes and no. Milk contains variable amounts of biotin so it should not be used with avidin/biotin detection systems. Milk also contains varying amounts of phosphoproteins that may make interfere with anti-phosphotyrosine procedures. A variety of blocking buffers are compatible with the substrate. Please see our Blocking Buffer selection table (https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/western-blot-buffers/blocking-buffers-western-blotting.html#table).

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Answer Id: E8509

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I need more ProcartaPlex Streptavidin-PE for my assay. Can I purchase this item separately? Product FAQ

Answer

Yes, ProcartaPlex Streptavidin-PE (Cat. No. EPX-SAPE-000) is available as a stand-alone item. We offer most of our PorcartaPlex buffers and reagents as stand-alone items. A complete list of what is currently available can be found on our website here: https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-assays-analysis/luminex-multiplex-assays/procartaplex-immunoassays/procartaplex-supplemental-products.html.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Answer Id: E16833

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Can any Gibco formulation be customized with the Gibco Media Configurator? Product FAQ

Answer

Almost all Gibco cell culture basal media products can be customized using the Gibco Media Configurator. To check on a particular product, use the Media Configurator page (https://www.thermofisher.com/us/en/home/life-science/bioproduction/custom-cell-culture-media-and-services/gibco-custom-media-configurator.html to search by Gibco catalog number, or browse our offering by description. For the customization of non-media Gibco products, such as buffers and growth factors, contact us at custommedia@lifetech.com.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Answer Id: E11844

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Do you recommend a specific method for preparing samples for immunoprecipitation (IP), co-immunoprecipitation (Co-IP), or pulldown assays? Product FAQ

Answer

Depending on your specific immunoprecipitation application and location of the target antigen within the cell (i.e., nucleus, cytosol, membrane, etc.,), we offer a variety of different lysis buffers, depending on the sample type and target (http://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-purification-isolation/cell-lysis-fractionation.html). You should develop an appropriate lysis strategy to maximize structural integrity. The ionic strength (salt concentration), choice of detergent, and pH of the lysis buffer may affect the immunoprecipitation efficiency as well as the integrity of the target antigen. Some common lysis buffers used include RIPA buffer and NP-40 buffer.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Answer Id: E12994

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How do I select the right blocking buffer for my Western blotting application? Product FAQ

Answer

Please review the table in this link (https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/western-blot-buffers.html) to pick the right Western blot blocking buffer for your specific need. Additionally, here are some general tips to keep in mind:

  • Milk-based blocking buffers are not compatible with biotin systems.
  • SuperBlock Blocking Buffers can cross-react with some antibodies raised against rabbits.
  • Blockers in phosphate-based buffers are generally not recommended in systems using alkaline phosphatase conjugates.
  • StartingBlock Buffers (Cat. Nos. 37538 and 37542) are recommended when the optimal blocking buffer has not been determined.


Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Answer Id: E19625

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What types of membrane work best with SuperSignal West Femto Maximum Sensitivity Substrate? Product FAQ

Answer

Most researchers use nitrocellulose or polyvinyldiflouride (PVDF) membranes with SuperSignal West Femto Maximum Sensitivity Substrate. Both work well, although nitrocellulose seems to be better suited in some applications than PVDF. In addition, charge-modified nylon membrane performs well with this substrate. Please also see our guide for choosing western blot membranes (https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/transfer-proteins-western-blot/membranes-transfer-buffers-western-blotting/membranes-western-blotting.html).

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Answer Id: E8518

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