Learn More Biochemicals
Biochemical refer to any chemical that is found in a biological system or that can be used for biological research. Biochemical compounds include molecules such as amino acids, vitamins, and nucleotides as well as organic and inorganic chemicals that have life science applications.
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Learn More Fatty Acids
A fatty acid is a carboxylic acid with a long, unbranched aliphatic chain. The aliphatic chain may be either saturated or unsaturated. Chains of even-numbered length predominate in nature. In biology, fatty acids are usually derived from triglycerides or phospholipids.
Specially designed cationic lipids, such as the Invitrogen Lipofectamine Transfection Reagents, facilitate DNA and siRNA delivery into cells (Chesnoy and Huang, 2000; Hirko et al., 2003; Liu et al., 2003).
(See a list of the products featured in this article) The generation of reactive oxygen species (ROS) is a natural consequence of aerobic energy metabolism. Oxidative stress occurs when the cell’s antioxidant capacity is unable to keep pace with the production of ROS.
Learn More Cationic Lipid Transfection
Cationic lipid-mediated transfection is one of the most popular methods for introducing foreign genetic material into cells. Although first generation of lipid-based transfection reagents relied on artificial liposomes that could envelop nucleic acids and then fuse with the cell membrane to deposit...
Learn More Hydrophobic, Lipid-Binding Plates
See our selection of lipid-binding and lipoprotein-binding microplates to maximize passive adsorption and coating capacity of hydrophobic biomolecules for immunoassay techniques., These polystyrene surfaces are less polar with high affinity to molecules of a more hydrophobic nature.
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Learn More Lipid-Based Transfection—Troubleshooting
Having difficulties with your experiment? We are dedicated to your success. Get back on track. View our expert recommendations for commonly encountered problem scenarios. View the relevant questions below:, Browse our FAQ database for more information ›, Need more information?
Lipases and inorganic phosphates are critical in many signaling pathways, from mTOR to GPCRs where they are involved in disease states ranging from cardiovascular to inflammation and cancer. Measuring phosphates and phosphatase activity in solution is fundamental to an understanding of cellular...
(See a list of the products featured in this article.) Alterations in lipid metabolism are known to be the root defect in atherosclerosis. Atherosclerosis is a form of arteriosclerosis in which the thickening and loss of elasticity of the artery walls is caused by the buildup of fatty...
Learn More Lipid-Based Transfection Support
For Research Use Only. Not for use in diagnostic procedures., What recommendations do you have for selecting a transfection reagent? What points should I consider to achieve optimal transfection with a lipid-based transfection reagent? What is the stability of your transfection reagents?
(See a complete list of products discussed in this article.) Time-lapse imaging of live cells labeled with fluorescent dyes is a powerful technique for interrogating cell biology. As compared with organic fluorophores, the bright and photostable Qdot® conjugates can provide higher-content imaging by...
Optimize your experiments to get the best results. We’ve compiled a detailed knowledgebase of the top tips and tricks to meet your research needs. View the relevant questions below:, Browse our FAQ database for more information ›, Need more information? Contact us › For Research Use Only.
The first step for successful transfections is to choose the best transfection reagent for your application. Further optimization may be necessary. The table below will help you address some of these important factors so that you can easily achieve superior transfection results.