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c-Myc p67 Monoclonal Antibody (9E10), eBioscience™ (Invitrogen™)

c-Myc Monoclonal Antibody for Western Blot, IHC (P), IP

c-Myc Tag Monoclonal Antibody (9E10) (Invitrogen™)

c-Myc Monoclonal Antibody for Western Blot, IF, ICC, IHC (F), IHC (P), Flow, IP, ChIP, ELISA

c-Myc Tag Monoclonal Antibody (9E10), Alexa Fluor 488 (Invitrogen™)

c-Myc Monoclonal Antibody for Western Blot

Pierce™ Anti-c-Myc Agarose (Thermo Scientific™)

Thermo Scientific Pierce Anti-c-Myc Agarose is a high-affinity immunoprecipitation resin ideal for purification of recombinant c-Myc-tagged proteins expressed in human in vitro expression systems and bacterial and mammalian cell lysates.

Features of Anti-c-Myc Agarose:

Specific—highly specific anti-c-Myc monoclonal antibody (clone 9E10) enables high yield and high purity for immunoprecipitation
Scalable—available in 2 and 10 mL resin package sizes to allow for larger scale purifications or immunoprecipitations
Versatile—can be used in spin or gravity columns as well as in FPLC cartridges
Convenient—reagents to elute and detect c-Myc tagged fusion proteins are available separately

The anti-c-Myc antibody used to manufacture Pierce Anti-c-Myc Agarose is a highly specific mouse IgG1 monoclonal antibody (clone 9E10) that recognizes the c-Myc epitope tag (EQKLISEEDL) derived from the human c-myc oncogene (p62 c-myc). The support is Superflow 6 resin, a highly crosslinked 6% agarose resin. This anti-c-Myc affinity resin can be packed into gravity purification columns, spin purification columns or cartridges for FPLC instruments to purify c-Myc-fusion proteins expressed in bacterial or mammalian cells.

Applications:
• Purification of c-Myc fusion proteins expressed in the Pierce Human In Vitro Translation kits
• Large scale purification of recombinant c-Myc tagged proteins
• High-throughput enrichment of fusion proteins and interacting partners
• IP and co-IP experiments (as with the Pierce c-Myc-Tag IP/co-IP Kit)

Pierce Anti-c-Myc Agarose consists of a highly specific monoclonal anti-c-Myc antibody that is covalently immobilized on a crosslinked 6% beaded agarose support. The product is supplied as a 25% slurry. Upon incubation with a sample, c-Myc tagged fusion protein is captured on the agarose beads. After simple washing steps, the tagged protein is easily eluted from the resin using 0.1M glycine (pH 2.0-2.8), 3M NaSCN or 50 mM NaOH depending on the downstream application of the purified protein. For elution of highly functional proteins, Pierce c-Myc-peptide can also be used to elute the c-Myc tagged protein. Anti-c-Myc antibody may be used to detect the presence of the tagged protein by Western blot.

In experiments with a c-Myc-tagged fusion protein (26 to 29kDa), the resin provided a binding capacity of 102 to 144nmol protein per mL of settled resin. The elution capacity was at least 18 to 19nmol per mL of settled resin using 0.1M glycine, pH 2.8. Binding and elution capacity will vary depending on the c-Myc-fusion protein and the method of elution.

Related Products
c-Myc Peptide Control for Pierce™ Anti-c-Myc Agarose

c-Myc Tag Monoclonal Antibody (9E10), Alexa Fluor 555 (Invitrogen™)

c-Myc Monoclonal Antibody for Western Blot, IF, ICC

pEF6/myc-His A, B, & C Mammalian Expression Vectors (Invitrogen™)

All pEF or pUB vectors contain a strong promoter for high-level expression in mammalian cells, a choice of selection marker for generating stable cell lines, and an epitope tag for easy detection with a monoclonal antibody and rapid purification on nickel-chelating resin. Each vector is available in three reading frames to simplify cloning in-frame with the fusion tag.

c-Myc Tag Monoclonal Antibody (9E10) (Invitrogen™)

c-Myc Monoclonal Antibody for Western Blot, IF, IHC (P), IP, ELISA, GS

T-REx™ Complete Kit, with pcDNA™4/TO/myc-His© Vector (Invitrogen™)

A Tetracycline-Regulated Expression System without Viral Transactivators
The T-REx™ System yields higher levels of induced expression than any other regulated mammalian expression system. It utilizes the complete CMV promoter and adds control elements from the bacterial tetracycline resistance operon to effectively repress and derepress transcription from one of the strongest mammalian promoter sequences known (1,2).

Specific Activation
The T-REx™ System uses a repressor mechanism that blocks transcription from the powerful CMV promoter in the absence of tetracycline. Because the T-REx™ System elements do not use viral transactivators, you can achieve high-level expression from the complete CMV promoter without secondary, non-specific activation of host genes.

The T-REx™ Mechanism
The T-REx™ transcriptional control elements are illustrated in Figure 1. Two tetracycline operator sequences (TetO2) have been inserted between the TATA box of the CMV promoter and the transcriptional start site. The TetO2 sequence itself has no effect on expression. When the tetracycline repressor protein (TR) is present, it effectively binds the TetO2 sites and blocks transcription initiation. Tetracycline added to the culture medium binds to, and changes the conformation of, the TR protein. This change causes the TR protein to release the TetO2 sites, derepressing transcription from the CMV promoter. The result is high-level expression of the gene of interest (Figure 2). Expression levels can be modulated based on the tetracycline concentration and can be induced to levels that are achieved with constitutive CMV expression vectors.
T-REx™ is a powerful inducible mammalian expression system that allows you to regulate expression from the complete human cytomegalovirus (CMV) enhancer-promoter. T-REx™inducible expression vectors offer the following features:

• Complete CMV enhancer-promoter sequence containing two copies of the tetracycline operator TetO2 sequence for high-level regulated expression
• Zeocin™ or hygromycin resistance gene for effective selection of stable mammalian cell lines
• Large multiple cloning site to simplify cloning

In addition, pcDNA™4/TO/myc-His offers a c-myc epitope for rapid detection of the recombinant protein with an Anti-myc Antibody and a polyhistidine (6xHis) sequence for simple purification of the recombinant protein with nickel-chelating resin and detection with Anti- His(C-term) Antibody.

The regulatory vector, pcDNA™6/TR, is provided for high-level expression of the tetracycline repressor (TR) protein. This vector expresses the Blasticidin resistance gene for rapid selection of mammalian cell lines that stably express the TR protein.

c-Myc Tag Monoclonal Antibody (289-19510) (Invitrogen™)

c-Myc Monoclonal Antibody for IF, ICC, IHC

n-Myc Monoclonal Antibody (NCM-II 100) (Invitrogen™)

n-Myc Monoclonal Antibody for Western Blot

c-Myc Tag Monoclonal Antibody (9E10), Alexa Fluor 647 (Invitrogen™)

c-Myc Monoclonal Antibody for Western Blot, IF, ICC

c-Myc Tag Monoclonal Antibody (9E10), FITC (Invitrogen™)

c-Myc Monoclonal Antibody for IF

pcDNA™4/TO/myc-His A, B, & C Mammalian Expression Vectors (Invitrogen™)

A Tetracycline-Regulated Expression System without Viral Transactivators
The T-REx™ System yields higher levels of induced expression than any other regulated mammalian expression system. It utilizes the complete CMV promoter and adds control elements from the bacterial tetracycline resistance operon to effectively repress and derepress transcription from one of the strongest mammalian promoter sequences known (1,2).

Specific Activation
The T-REx™ System uses a repressor mechanism that blocks transcription from the powerful CMV promoter in the absence of tetracycline. Because the T-REx™ System elements do not use viral transactivators, you can achieve high-level expression from the complete CMV promoter without secondary, non-specific activation of host genes.

The T-REx™ Mechanism
The T-REx™ transcriptional control elements are illustrated in Figure 1. Two tetracycline operator sequences (TetO2) have been inserted between the TATA box of the CMV promoter and the transcriptional start site. The TetO2 sequence itself has no effect on expression. When the tetracycline repressor protein (TR) is present, it effectively binds the TetO2 sites and blocks transcription initiation. Tetracycline added to the culture medium binds to, and changes the conformation of, the TR protein. This change causes the TR protein to release the TetO2 sites, derepressing transcription from the CMV promoter. The result is high-level expression of the gene of interest (Figure 2). Expression levels can be modulated based on the tetracycline concentration and can be induced to levels that are achieved with constitutive CMV expression vectors.
T-REx™ is a powerful inducible mammalian expression system that allows you to regulate expression from the complete human cytomegalovirus (CMV) enhancer-promoter. T-REx™inducible expression vectors offer the following features:

• Complete CMV enhancer-promoter sequence containing two copies of the tetracycline operator TetO2 sequence for high-level regulated expression
• Zeocin™ or hygromycin resistance gene for effective selection of stable mammalian cell lines
• Large multiple cloning site to simplify cloning

In addition, pcDNA™4/TO/myc-His offers a c-myc epitope for rapid detection of the recombinant protein with an Anti-myc Antibody and a polyhistidine (6xHis) sequence for simple purification of the recombinant protein with nickel-chelating resin and detection with Anti- His(C-term) Antibody.

The regulatory vector, pcDNA™6/TR, is provided for high-level expression of the tetracycline repressor (TR) protein. This vector expresses the Blasticidin resistance gene for rapid selection of mammalian cell lines that stably express the TR protein.

c-Myc Tag Polyclonal Antibody (Invitrogen™)

Myc Tag Polyclonal Antibody for Western Blot, IF, ICC

pEF1/myc-His A, B, & C Mammalian Expression Vectors (Invitrogen™)

All pEF or pUB vectors contain a strong promoter for high-level expression in mammalian cells, a choice of selection marker for generating stable cell lines, and an epitope tag for easy detection with a monoclonal antibody and rapid purification on nickel-chelating resin. Each vector is available in three reading frames to simplify cloning in-frame with the fusion tag.