Product FAQ

Are the antibodies on your Dynabeads magnetic beads for Cell Isolation/Activation/Expansion covalently bound to the beads?

Answer

Yes. The antibodies are covalently bound and thus should be very stable in your applications.

Answer Id: E4656

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Product FAQ

When expanding naive T cells from MNC without prior T cell isolation, will the other MNCs grow along with the T cells?

Answer

No, only T cells will get the stimulation signal and grow in culture. After a week the purity of CD3+ cells is typically above 95%. Stimulating MNC can be problematic due to the presence of monocytes as they will phagocytose the beads. We recommend that you remove the monocytes from the MNC or to isolate T cells before T cell activation/expansion by standard methods such as freeze/thaw, plastic adherence, or use of Dynabeads CD14 (Cat. No. 11149D, for human RUO).

Answer Id: E12150

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Product FAQ

What Dynabeads magnetic bead size should I select?

Answer

This will depend on your application. As a guideline, the 4.5 micron beads are best used for cell isolation and activation/expansion. These larger beads have a higher magnetic mobility, they are roughly the same size as mammalian cells, and are less likely to be taken up by the cells. The smaller 1 micron beads and 2.8 micron beads are often used when isolating nucleic acids or proteins, or for immunoprecipitation. In negative cell isolation kits, one micron beads are often used because of their higher binding capacity per milliliter of beads and faster binding kinetics. With negative selection, cells taking up any beads will not be a problem as you want to look at the remaining cell population anyway. The 2.8 micron Dynabeads magnetic beads, coated with secondary antibodies, protein A or protein G, or streptavidin are also used for positive cell isolation with primary antibodies of your own choice, targeting specific cell-surface antigens.

Answer Id: E12080

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Product FAQ

What are the differences between Dynabeads Human T-Activator CD3/CD28 beads and Dynabeads Human T-Expander CD3/CD28 beads?

Answer

Dynabeads Human T-Activator CD3/CD28 beads (Cat. Nos. 111-31D, 111-32D, 111-61D) are used for activation and expansion of all human T cells expressing CD3.

Dynabeads Human T-Expander CD3/CD28 beads(Cat. No. 111-41D) are used for isolation and expansion of human T cells due to its higher affinity antibodies coupled to the Dynabeads magnetic beads. It uses a research grade of Dynabeads CD3?CD28 CTS beads (Cat. No. 402-03D)

These two products thus differ in the antibody clones used and in the ratio of anti-CD3 and anti-CD28 antibodies.

Dynabeads Human T-Activator CD3/CD28 beads provides a weaker signal than Dynabeads Human T-Expander CD3/CD28 beads. Because it uses antibodies with lower affinity than those in Dynabeads Human T-Expander CD3/CD28 beads, it cannot be used for isolation.

Answer Id: E6211

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Product Literature

Biomarker Quantitation Assay Guide

Citations & References

Flow-cytometric isolation of human antibodies from a nonimmune Saccharomyces cerevisiae surface display library.

  • Authors: Feldhaus MJ, Siegel RW, Opresko LK, Coleman JR, Feldhaus JM, Yeung YA, Cochran JR, Heinzelman P, Colby D, Swers J, Graff C, Wiley HS, Wittrup KD
  • Journal: Nat Biotechnol (2003) 21:163-170
  • PubMed ID: 12536217
Catalog #

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Brochure: Protein Quantitation Using Mass Spectrometry

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User's Guide: Amira Software 2019

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Cell therapy research product selection guide

Manual / Product Insert

User Guide: Drosophila Expression System (DES)

Version: MAN0000051 Rev A.0 (29 January 2016)
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