Media Formulation

12330 - McCoy's 5A (modified), HEPES

Catalog #

Product FAQ

Is it necessary to store DMEM, high glucose, pyruvate medium in the dark?

Answer

Yes, the medium should be stored in the dark because there are some components in the medium such as HEPES, Tryptophan, and Riboflavin, etc. that are sensitive to light.

Answer Id: E13291

Was this answer helpful?

Yes
No
Thank you for your response

Product FAQ

What is the shelf life of the DMEM, high glucose, pyruvate medium once the bottle is opened and the medium is supplemented?

Answer

We suggest storing the supplemented media at 28 degrees C and using it within 2-4 weeks.

Answer Id: E13292

Was this answer helpful?

Yes
No
Thank you for your response

Product FAQ

Besides DMEM, what other media contain pyridoxine HCL?

Answer

RPMI-1640, Nutrient Mix (Ham's) F-10 and F-12, Leibovitz's L-15, McCoy's 5A, MCDB 131, Media 199, F-12K (Kaighn's Modification), NCTC-109, and Waymouth's Medium all contain the pyridoxine HCl form of vitamin B6.

Answer Id: E3016

Was this answer helpful?

Yes
No
Thank you for your response

Citations & References

Intracellular mRNA cleavage induced through activation of RNase P by nuclease-resistant external guide sequences.

  • Authors: Ma M; Benimetskaya L; Lebedeva I; Dignam J; Takle G; Stein C A;
  • Journal: Nat Biotechnol (2000) 18:58-61
  • PubMed ID: 10625392

Manual / Product Insert

Tango GPR39-bla U2OS Cell-based Assay

Version: 6 November 2010
Catalog #

Product Literature

Application Note: Phenotypic fingerprint of cellular function following gene editing by high-throughput imaging acquisition and analysis

Manual / Product Insert

Tango GALR1-bla U2OS DA and Dividing Cell-based Assay

Version: 7 November 2010
Catalog #
  • K1616
  • K1617(Discontinued)

Manual / Product Insert

Tango NPY5R-bla U2OS DA and Dividing Cell-based Assay

Version: 28 October 2010
Catalog #
  • K1782
  • K1774(Discontinued)

Manual / Product Insert

CellSensor p53RE-bla HCT-116 Cell-based Assay Protocol

Version: 28 October 2010
Catalog #

Citations & References

B-Raf is dispensable for K-Ras-mediated oncogenesis in human cancer cells.

  • Authors: Kim JS, Lee C, Foxworth A, Waldman T,
  • Journal: Cancer Res (2004) 64:1932-1937
  • PubMed ID: 15026326

Manual / Product Insert

Tango CXCR1-bla U2OS cells

Version: 30 October 2010
Catalog #