Documents & Support

Technical note: Foreword to the Microbiological Media Technical Manual Product Literature

Technical note: Overview Thermo Scientific Microbiology Media Product Literature

Are IPTG and X-gal necessary for blue/white screening of colonies? Product FAQ

Answer

With a vector containing the lac promoter and the LacZ alpha fragment (for alpha-complementation), blue/white screening can be used as a tool to select for presence of an insert. X-gal is added to the plate as a substrate for the LacZ-encoded beta-galactosidase enzyme and must always be present to see the blue color in the blue/white screening.

In cases where the lacIq repressor is present (either provided by the host cells on an F' episome, i.e. TOP10F', or expressed from the plasmid), it will repress expression of LacZ from the lac promoter and prevent the formation of the blue color with X-gal. This repression can be reversed by adding IPTG to the media in addition to X-gal, which will inhibit the action of lacIq and re-activate expression from the lac promoter.

Answer Id:: E3322

Was this answer helpful?

Yes No

Thank you for your response

What bacterial growth media should I use? Product FAQ

Answer

Check out our bacterial growth media chart (https://www.thermofisher.com/us/en/home/life-science/cell-culture/microbiological-culture/bacterial-growth-media.html).

Answer Id:: E6717

Was this answer helpful?

Yes No

Thank you for your response

I wanted to double-feed the iPSC culture on Friday with StemFlex Medium, but on Saturday, I realized that I forgot about it. What should I do? Product FAQ

Answer

In such circumstances, we recommend either full media change on Sunday or overlaying the culture with additional media on Sunday.

Answer Id:: E14758

Was this answer helpful?

Yes No

Thank you for your response

Do you offer assistance with media formulation development? Product FAQ

Answer

Yes. Our Gibco Custom Media Specialists and R&D staff can provide advice related to the addition or elimination of media components. For more comprehensive services, our PD-Direct Bioprocess Services team specializes in media development and optimization.

Answer Id:: E11855

Was this answer helpful?

Yes No

Thank you for your response

What is the depth of the AlgiMatrix Matrix sponge? Will the sponge enlarge after addition of media? Product FAQ

Answer

The height of the sponge is ~3-4 mm dry. When the sponge is hydrated, its volume is essentially the same - it doesn't swell and increase in size, in fact it becomes slightly bit smaller as it essentially goes back into a hydrogel consistency, especially if the inoculated sponges are centrifuged briefly (as suggested to force cells more into the interior and also to spread the sponge uniformly over the bottom of the well).

Answer Id:: E11799

Was this answer helpful?

Yes No

Thank you for your response

Are there any additional components that need to be added to the media of the PSC Definitive Endoderm Induction Kit? Product FAQ

Answer

No other components are required.

Answer Id:: E9380

Was this answer helpful?

Yes No

Thank you for your response

What is the difference between Advanced RPMI 1640 Medium and other RPMI 1640 formulations? Product FAQ

Answer

Advanced Media are widely used basal media, which require 50-90% less Fetal Bovine Serum (FBS) supplementation compared to conventional basal media formulations. The Advanced media is unique due to the addition of the following ingredients to allow for serum reduction: ethanolamine, glutathione, ascorbic acid, insulin, transferrin, AlbuMAX I lipid-rich bovine serum albumin for cell culture, and the trace elements sodium selenite, ammonium metavanadate, cupric sulfate, and manganous chloride.

Answer Id:: E13252

Was this answer helpful?

Yes No

Thank you for your response

What is the difference between Advanced DMEM/F12 Medium and DMEM/F12 Medium? Product FAQ

Answer

Advanced Media are widely used basal media, which require 50-90% less Fetal Bovine Serum (FBS) supplementation compared to conventional basal media formulations. The Advanced media is unique due to the addition of the following ingredients to allow for serum reduction: ethanolamine, glutathione, ascorbic acid, insulin, transferrin, AlbuMAX I lipid-rich bovine serum albumin for cell culture, and the trace elements sodium selenite, ammonium metavanadate, cupric sulfate, and manganous chloride.

Answer Id:: E13249

Was this answer helpful?

Yes No

Thank you for your response

What is the difference between Advanced MEM Medium and other MEM formulations? Product FAQ

Answer

Advanced Media are widely used basal media, which require 50-90% less Fetal Bovine Serum (FBS) supplementation compared to conventional basal media formulations. The Advanced media is unique due to the addition of the following ingredients to allow for serum reduction: ethanolamine, glutathione, ascorbic acid, insulin, transferrin, AlbuMAX I lipid-rich bovine serum albumin for cell culture, and the trace elements sodium selenite, ammonium metavanadate, cupric sulfate, and manganous chloride.

Answer Id:: E13251

Was this answer helpful?

Yes No

Thank you for your response

What is the difference between Advanced DMEM Medium and other DMEM formulations? Product FAQ

Answer

Advanced Media are widely used basal media, which require 50-90% less Fetal Bovine Serum (FBS) supplementation compared to conventional basal media formulations. The Advanced media is unique due to the addition of the following ingredients to allow for serum reduction: ethanolamine, glutathione, ascorbic acid, insulin, transferrin, AlbuMAX I lipid-rich bovine serum albumin for cell culture, and the trace elements sodium selenite, ammonium metavanadate, cupric sulfate, and manganous chloride.

Answer Id:: E13250

Was this answer helpful?

Yes No

Thank you for your response

I have cells in culture. Do I need to wash the cells and remove the culture media prior to starting the MagniSort protocol? Product FAQ

Answer

Cells can be stained with the antibody or antibody cocktails in the culture media; however, subsequent washes should be performed with the recommended cell separation buffers before the addition of magnetic beads. Culture media such as RPMI and DMEM interfere with the binding of magnetic beads to target cells.

Answer Id:: E14632

Was this answer helpful?

Yes No

Thank you for your response

Do I need to change the media after the ExpiFectamine CHO transfection? Product FAQ

Answer

No. The ExpiCHO Expression System is designed to run without media exchanges. There is no need to remove transfection complexes or to change growth medium following transfection, however there are an enhancer addition and 1-2 optional feed additions for improved yield.

Answer Id:: E11549

Was this answer helpful?

Yes No

Thank you for your response

Results per page
    spinner