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Can you provide the shelf-life for the Pierce Microplate BCA Protein Assay Kit-Reducing Agent Compatible? Product FAQ

Answer

The Pierce Microplate BCA Protein Assay Kit-Reducing Agent Compatible is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended (room temperature). Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf) for more details.

Answer Id:: E19664

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SH-3000 Rotor Product Literature

Optimization of a bulk reagent liquid handler for low volume dispensing Product Literature

Which Nunc products can I use for high-throughput, plate-based DNA plasmid minipreps? Product FAQ

Answer

We recommend using the following Nunc products:

- 96-Well Polypropylene DeepWell Storage Plate, 2 mL (Cat. No. 278743) (sterile growth plate)
- Microplate Lid for 96 microwell plate (Cat. No. 264122) (to cover plate during growth)
- Polyolefin Acrylate Sealing Tape (Cat. No. 232701) (to cover plate during mixing)
- Fritted 96-Well Deep Well Filter Plate (Cat. No. 278011) (for lysate clarification)
- Fritted 96-Well Deep Well Filter Plate with Glass Fiber Filter (Cat. No. 278010) (for DNA binding)
- 96-Well Polypropylene DeepWell Storage Plate, 1.3 mL (Cat. No. 260252) (reception plate)

Answer Id:: E17511

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For my enzyme activity assay, I covered my microplates with an adhesive sealant sheet, incubated my samples and then collected all liquid that may have condensed on the surface of the plastic seal by centrifugation. I noticed the results from these samples are different from my colleague who does not seal microplates during incubation, but only covers them with a plastic lid. What could cause the discrepancies in our assays? Product FAQ

Answer

The droplets of water that collected at the surface of the sealant sheet may have picked up chemicals or other contaminants in the adhesive. 

Answer Id:: E15868

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Are any parts of Wellwash and Wellwash Versa autoclavable? Product FAQ

Answer

The following parts are autoclavable: cell wash head, liquid containers, liquid container caps, intake tubes, priming vessel, tube connectors.
The following parts are NOT AUTOCLAVABLE: aerosol cover, blank wash head, liquid filters, liquid level sensor assemblies, sensor nut, 1x8 wash heads, 1x12 wash heads, 1x16 wash heads.

Answer Id:: E16694

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If the protein/enzyme cannot be isolated in pure form, is the assay still valid? Product FAQ

Answer

Not all proteins/enzymes can be isolated in pure form. They may require accessory proteins or chaperonins for activity or require that their hydrophobic domains are covered with lipids, such as with membrane proteins/enzymes. Even proteins/enzymes isolated by affinity purification may co-elute with other cellular components that do not dissociate easily. 

Validity is established by understanding what else is bound to the protein/enzyme of interest and how those other components can affect the assay. Ideally, the material should be analyzed by native and/or SDS-PAGE as well as other analytical methods to determine total protein concentration and the concentrations of other components (e.g., lipid content, polysaccharide content, metal ion content, etc.).

If your sample is hydrophobic, it may bind to any lipids associated with the protein/enzyme even though these never enter the active or binding site. Also, hydrophobic components and interactions may cause the compound to bind to the surfaces of microplate wells or cuvettes. A direct assay may help with such samples. 

If the presence or absence of an impurity affects the activity or binding properties of a protein/enzyme, different lots of the protein may vary in the amount/ratios of these impurities may result in variations in the final results. 

Answer Id:: E15847

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Comparison of methods for monitoring bacterial transport in the subsurface. Citations & References

  • Authors: DeFlaun MF, Fuller ME, Zhang P, Johnson WP, Mailloux BJ, Holben WE, Kovacik WP, Balkwill DL, Onstott TC
  • Journal: J Microbiol Methods
  • PubMed ID: 11576686
Catalog #

Cultivable bacterial composition and BIOLOG catabolic diversity of biofilm communities developed on Phragmites australis Citations & References

  • Authors: Rusznyak, A; Vladar, P; Molnar, P; Reskone, MN; Kiss, G; Marialigeti, K; Borsodi, AK
  • Journal: AQUATIC BOTANY

Neurobiology ELISA Protocol Experiment Protocol

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