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Uneven illumination occurs when the light does not evenly illuminate your sample across the field of view. This results in darker areas and more brightly illuminated areas, as you can see below. Often you’ll see places (usually in the corners) that are darker than the rest of the image.

See it fast and identify it faster with the Nicolet RaptIR+/RaptIR FTIR Microscope, The versatility of the RaptIR+/RaptIR allows you to analyze across a wide range of applications: Basic analysis—Work to identify contamination in production materials to find the origin of embedded particles...

By placing your sample on the stage and directing light through it, the filters, detectors, and camera, you will be able to visualize and capture an image of your sample. Learn about basic light path and filter configurations, what governs the limit of resolution, and the differences between upright...

Introduction to cryo-EM Sample preparation Introduction to the microscope Image formation and aberrations, Accelerator Condenser lens and parallel illumination Objective, lens, stage, and projection Detectors and cameras, Single particle analysis data collection and EPU Entire workflow uninterrupted...

Introduction to cryo-EM Sample preparation Introduction to the microscope Image formation and aberrations, Accelerator Condenser lens and parallel illumination Objective, lens, stage, and projection Detectors and cameras, Single particle analysis data collection and EPU Entire workflow uninterrupted...

Nicolet iN5 FTIR overview, Quickly identify unknown materials and contaminants that may affect product quality. The Thermo Scientific Nicolet iN5 FTIR Microscope provides rapid identification with point-and-shoot simplicity. The no-nonsense workflow requires only a few short steps.

(See a list of the products featured in this article.) Advanced fluorescence techniques demand quality at every level, from specimen preparation and fluorophore choice to instrument set-up and calibration.

Introduction to cryo-EM Sample preparation Introduction to the microscope Image formation and aberrations, Accelerator Condenser lens and parallel illumination Objective, lens, stage, and projection Detectors and cameras, Single particle analysis data collection and EPU Entire workflow uninterrupted...

Microscopes are powerful research and discovery tools and have contributed to countless ground-breaking discoveries, over several centuries. Find basic definitions for common microscopy terms, information to help you understand differences between magnification and resolution, and how fluorescence...

Microscopes are powerful research and discovery tools and have contributed to countless ground-breaking discoveries, over several centuries. Find basic definitions for common microscopy terms, information to help you understand differences between magnification and resolution, and how fluorescence...

Cells can become damaged from both the low- and high-wavelength light that is used to excite fluorophores. Most cells used in research are not adapted to handle the number of photons that are directed toward them during a typical imaging experiment.

Introduction to cryo-EM Sample preparation Introduction to the microscope Image formation and aberrations, Accelerator Condenser lens and parallel illumination Objective, lens, stage, and projection Detectors and cameras, Single particle analysis data collection and EPU Entire workflow uninterrupted...

Introduction to cryo-EM Sample preparation Introduction to the microscope Image formation and aberrations, Accelerator Condenser lens and parallel illumination Objective, lens, stage, and projection Detectors and cameras, Single particle analysis data collection and EPU Entire workflow uninterrupted...

Introduction to cryo-EM Sample preparation Introduction to the microscope Image formation and aberrations, Accelerator Condenser lens and parallel illumination Objective, lens, stage, and projection Detectors and cameras, Single particle analysis data collection and EPU Entire workflow uninterrupted...

Introduction to cryo-EM Sample preparation Introduction to the microscope Image formation and aberrations, Accelerator Condenser lens and parallel illumination Objective, lens, stage, and projection Detectors and cameras, Single particle analysis data collection and EPU Entire workflow uninterrupted...