Product Literature

SuperFrost and SuperFrost Printink Microscope Slides

Product Literature

Microscope Slides and Specialty Glass Field Reference Guide

Manual / Product Insert

FluoCells Prepared Microscope Slides

Version: 02-04-2009
Catalog #

Citations & References

Automated image analysis of live/dead staining of the fungus Aureobasidium pullulans on microscope slides and leaf surfaces.

  • Authors: Nelson CD, Spear RN, Andrews JH
  • Journal: Biotechniques (2000) 29:874-80, 882
  • PubMed ID: 11056819

Product Literature

Brochure: Microscope Slides Brochure

Product FAQ

What mounting media should I use with Qdot nanocrystals?

Answer

Qdot nanocrystals do not require the use of antifades as they do not photobleach or fade in the same manner as a chemical dye. In our studies, Qdot nanocrystals work best with the following mountants:

- HistoMount medium (Cat No. 00-8030); best for long-term archiving
- Cytoseal 60 Mountant
- Clarion Mountant
- Most polyvinyl alcohol-based mountants (limited storage time, less than weeks)
- Water-based mountants (limited storage time, less than a week)
- Up to 50% glycerol (limited storage time, less than a week)
Note: We do not recommend using ProLong mounting media with Qdot nanocrystals.

Answer Id: E12225

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Product FAQ

What is the cell culture treatment performed on glass Lab-Tek slides?

Answer

The standard glass Lab-Tek slide cell culture treatment consists of a proprietary, multi-stage high-purity water washing process. Unlike our plastic products for adherent cell culture (see Nunclon Delta), there is no need for further modification of the surface because glass is already hydrophilic and therefore already suitable for culture of many adherent cell lines.

Answer Id: E15933

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Product FAQ

What are the different options available in Lab-Tek chamber slides?

Answer

Lab-Tek chambered slide systems are based either on a standard soda lime glass microscope slide, or a culture-treated Permanox plastic slide. The latter may serve as a better culture surface for certain types of cells that don’t grow well on glass, but may be of limited use for certain types of fluorescence microscopy due to the endogenous autofluorescence of plastic.

Answer Id: E15934

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Product FAQ

What are the different options available in Lab-Tek II chamber slides?

Answer

Lab-Tek II chambered glass slide systems are available in our standard high-purity water washed culture treatment or our “CC2” surface modification. The latter is a proprietary passive treatment that resembles poly-D-lysine, often improving culture performance when working with fastidious cells such as neuronal cultures.

Answer Id: E15935

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Product FAQ

What are the differences between Lab-Tek and Lab-Tek II chamber slide systems?

Answer

The main difference is the way the upper chamber structure is attached to the slide. Lab-Tek slides use a silicone gasket that can be peeled away once the chambers are removed after culture. Lab-Tek II slides use a hydrophobic acrylic adhesive that remains on the slide after chamber removal but (due to its very low profile) will not interfere with coverslips. The chamber geometry is also slightly different: on Lab-Tek slides the walls lean inward slightly to limit media evaporation, whereas on Lab-Tek II slides they are completely vertical.

Answer Id: E15932

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Product FAQ

What stains and fixatives are compatible with Lab-Tek chambered slides and coverglasses?

Answer

Lab-Tek products are compatible with all common fixatives, including paraformaldehyde. Acetone at 100% and some mixtures of acetone and alcohols are not compatible with the polystyrene upper well structure and will result in clouding or other effects of chemical attack. We encourage careful testing within planned operating conditions before working with high-value samples.

Answer Id: E15937

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Product FAQ

What factors must I consider when working with Lab-Tek and Lab-Tek II chambered coverglass products?

Answer

Lab-Tek chambered coverglasses are not accessories to Lab-Tek chamber slides, but another complete product for cell culture. The chambers are simply adhered to a borosilicate coverglass (to serve as the culture surface) rather than a soda lime glass or Permanox microscope slide. Most customers select this format for compatibility with confocal imaging systems that require a #1.0, approx. 120 micron (Lab-Tek) or #1.5, approx. 180 micron (Lab-Tek II) substrate. Because the coverglass is very thin and fragile, the chambered coverglasses (unlike chamber slides) cannot be disassembled.

Answer Id: E15936

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Product Literature

Gold Seal Glass

Product FAQ

Which FFPE sample types are suitable for Direct PCR from FFPE tissues?

Answer

FFPE tissue, FFPE tissue sections, tissue blocks and microscope slides can all be used in sample preparation.

Answer Id: E8711

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Product FAQ

What is a Piko PCR Plate?

Answer

A Piko PCR Plate is a plate for PCR that is equivalent to one-fourth of a standard microplate and is equal to a microscope slide in footprint. These plates conserve all of the standard well-spacing and well-geometry of conventional microplates, e.g., a 96-well Piko PCR Plate has the same 4.5 mm pitch as most conventional 384-well microplates. Up to four Piko PCR Plates can be reversibly snapped into a frame to give the equivalent footprint of a conventional 96-well or 384-well microplate. Piko PCR Plates provide many advantages over traditional microplates: they are available at a lower cost, they accept lower reaction volumes, and require less storage space. In addition, many of the same types of advantages were incorporated in the instrumentation designed to use these plates: the thermal cycler has a smaller footprint, is less expensive, has higher thermal performance specifications, and consumes less power.

Answer Id: E8696

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