Product Literature

SuperFrost and SuperFrost Printink Microscope Slides

Product Literature

Brochure: Microscope Slides Brochure

Citations & References

Automated image analysis of live/dead staining of the fungus Aureobasidium pullulans on microscope slides and leaf surfaces.

  • Authors: Nelson CD, Spear RN, Andrews JH
  • Journal: Biotechniques (2000) 29:874-80, 882
  • PubMed ID: 11056819

Manual / Product Insert

FluoCells Prepared Microscope Slides

Version: 02-04-2009
Catalog #

Product Literature

Microscope Slides and Specialty Glass Field Reference Guide

Product FAQ

What mounting media should I use with Qdot nanocrystals?

Answer

Qdot nanocrystals do not require the use of antifades as they do not photobleach or fade in the same manner as a chemical dye. In our studies, Qdot nanocrystals work best with the following mountants:

- HistoMount medium (Cat No. 00-8030); best for long-term archiving
- Cytoseal 60 Mountant
- Clarion Mountant
- Most polyvinyl alcohol-based mountants (limited storage time, less than weeks)
- Water-based mountants (limited storage time, less than a week)
- Up to 50% glycerol (limited storage time, less than a week)
Note: We do not recommend using ProLong mounting media with Qdot nanocrystals.

Answer Id: E12225

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Product FAQ

What is the cell culture treatment performed on glass Lab-Tek slides?

Answer

The standard glass Lab-Tek slide cell culture treatment consists of a proprietary, multi-stage high-purity water washing process. Unlike our plastic products for adherent cell culture (see Nunclon Delta), there is no need for further modification of the surface because glass is already hydrophilic and therefore already suitable for culture of many adherent cell lines.

Answer Id: E15933

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Product FAQ

What are the different options available in Lab-Tek chamber slides?

Answer

Lab-Tek chambered slide systems are based either on a standard soda lime glass microscope slide, or a culture-treated Permanox plastic slide. The latter may serve as a better culture surface for certain types of cells that don’t grow well on glass, but may be of limited use for certain types of fluorescence microscopy due to the endogenous autofluorescence of plastic.

Answer Id: E15934

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Product FAQ

What are the different options available in Lab-Tek II chamber slides?

Answer

Lab-Tek II chambered glass slide systems are available in our standard high-purity water washed culture treatment or our “CC2” surface modification. The latter is a proprietary passive treatment that resembles poly-D-lysine, often improving culture performance when working with fastidious cells such as neuronal cultures.

Answer Id: E15935

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Product FAQ

What stains and fixatives are compatible with Lab-Tek chambered slides and coverglasses?

Answer

Lab-Tek products are compatible with all common fixatives, including paraformaldehyde. Acetone at 100% and some mixtures of acetone and alcohols are not compatible with the polystyrene upper well structure and will result in clouding or other effects of chemical attack. We encourage careful testing within planned operating conditions before working with high-value samples.

Answer Id: E15937

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Product FAQ

What are the differences between Lab-Tek and Lab-Tek II chamber slide systems?

Answer

The main difference is the way the upper chamber structure is attached to the slide. Lab-Tek slides use a silicone gasket that can be peeled away once the chambers are removed after culture. Lab-Tek II slides use a hydrophobic acrylic adhesive that remains on the slide after chamber removal but (due to its very low profile) will not interfere with coverslips. The chamber geometry is also slightly different: on Lab-Tek slides the walls lean inward slightly to limit media evaporation, whereas on Lab-Tek II slides they are completely vertical.

Answer Id: E15932

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Product FAQ

What factors must I consider when working with Lab-Tek and Lab-Tek II chambered coverglass products?

Answer

Lab-Tek chambered coverglasses are not accessories to Lab-Tek chamber slides, but another complete product for cell culture. The chambers are simply adhered to a borosilicate coverglass (to serve as the culture surface) rather than a soda lime glass or Permanox microscope slide. Most customers select this format for compatibility with confocal imaging systems that require a #1.0, approx. 120 micron (Lab-Tek) or #1.5, approx. 180 micron (Lab-Tek II) substrate. Because the coverglass is very thin and fragile, the chambered coverglasses (unlike chamber slides) cannot be disassembled.

Answer Id: E15936

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Product Literature

Gold Seal Glass

Product FAQ

Which FFPE sample types are suitable for Direct PCR from FFPE tissues?

Answer

FFPE tissue, FFPE tissue sections, tissue blocks and microscope slides can all be used in sample preparation.

Answer Id: E8711

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Product FAQ

Is there is gentle fixation method for preparing mouse and rat tissues?

Answer

Many of our antibodies directed to rat and mouse cell surface antigens have been shown to work well in immunohistochemistry with cryostat sections and paraffin-embedded sections of tissues fixed by the PLP (Paraformaldehyde-Lysine-Periodate) fixation method. The methods for preparing the cryostat sections and the paraffin embedded sections are described in detail in the following publication: Whiteland, J.L., et al. (1995) Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies, J. Histochem. Cytochem. 43(3):313-320.

The method for preparing cryostat sections is summarized as follows: Frozen specimens were embedded in Tissue Tek OCT. Serial 6 micrometer sections were then cut on a cryostat and placed on poly-L-lysine coated slides. The slides were dried at RT for 2-3 hours and stored desiccated at –70° C.

The method for preparing paraffin sections using the P-L-P fixation method is as follows: First, prepare a solution of 0.2 M L- lysine by adding the appropriate amount of L-lysine monohydrochloride to deionized water. Mix the desired volume of 0.2 M lysine with an equal volume of 0.1 M disodium hydrogen orthophosphate and then adjust the pH to 7.4 with HCl. This buffer can be stored at 4° C. The required paraformaldehyde-dextrose solution is made by first heating a 5% (w/v) dextrose solution to 60° C and then adding paraformaldehyde to a final concentration of 8% (w/v) while stirring. This is followed by 1 M NaOH added dropwise to aid dissolution of the paraformaldehyde. After cooling to room temperature, this solution can be stored at 4° C for a maximum of 2 months. The actual PLP fixative was prepared immediately before use. Solid sodium m-periodate is added to the lysine phosphate buffer to a final concentration of 0.02 M. Paraformdehyde-dextrose solution was then added to produce a final concentration of 0.25% paraformaldehyde.

Tissues (spleens in this case) were removed and cut into 2 mm or 3 mm pieces. The tissue pieces were fixed in 10 mL of PLP fixative for 24 hours at 4° C. After PLP fixation, the tissues were washed in PBS (three times for 5 minutes each). The tissues were rapidly dehydrated at 4° C through 70% ethanol (45 minutes), 90% ethanol (45 minutes), and 100% ethanol (twice for 30 minutes), then cleared at 4° C in HistoClear. The specimens were then infiltrated with low melting temperature paraffin (51-53° C) at 54° C for 30 minutes and then oriented and blocked in conventional paraffin. The paraffin blocks were brought to room temperature and cut into 6 mm sections on a microtome. The sections were floated on water at 40° C and then transferred to glass microscope slides pre-coated with poly-L-lysine. The slides were dried overnight at 37° C, wrapped in aluminum foil, and stored desiccated at –20° C. Immunohistochemical staining using unlabeled antigen-specific primary antibodies followed by HRP-labeled secondary antibodies is then performed in the normal way. The antigens are stained with either DAB (brown to black staining) or AEC (red staining).

On our website, you can find a collection of links to advice and other protocols for IHC. Check out links from our Technical Resources page, and you can also check out links on the following page: Products & Services > Cell Analysis > Cell Imaging > IHC.

Answer Id: E5183

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